FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9504_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9504_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1375860
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTCTCGTTACAGTAACT45720.3323012515808294No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA30590.22233366766967572No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA27750.20169203261959792No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT26070.18948148794208713No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA25930.18846394255229457No Hit
CTTATGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC25830.18773712441672846No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC23620.17167444362071724No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA21700.15771953541784775No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA21440.15582980826537585No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT21440.15582980826537585No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT21280.15466689924847005No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT20400.1482708996554882No Hit
CCTCTATTCTCTTACAATCCACAAACAGAAGTCCTAACTATATGCGGCAG19710.143255854520082No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19350.14063930923204396No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT19310.14034858197781752No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA19060.13853153663890222No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC18720.13606035497797742No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATATATCACCCAGG18260.13271699155437328No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT18250.13264430974081665No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG17950.13046385533411828No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG17640.12821071911386334No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA17530.1274112191647406No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTT17330.12595758289360837No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG17290.1256668556393819No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA17040.12384981030046661No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA16620.12079717413108891No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC16560.12036108324974926No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA16400.11919817423284346No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT16340.11876208335150379No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC16260.11818062884305089No Hit
GTATAGCACCTGTCTTGTTTTCCAACAAAATAGCCAGATTAGGGAAAGGA15740.11440117453810708No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA15670.1138924018432108No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC15610.11345631096187112No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT15380.11178462925006906No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG15250.1108397656738331No Hit
GTCTATAGCTGTCAGACATGATTTTAAAGGTTCAGTATCTATCACGGTCT15050.10938612940270084No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG15020.10916808396203101No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT14930.10851394764002152No Hit
GTCTAGATATTGTTTGTCCAAGTCTAAAGTCAATTCCCTTTTCAACATCT14880.10815053857223846No Hit
GTTCATAGAAATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATAT14820.10771444769089879No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT14430.10487985696219092No Hit
TTCTAATACTTTACTTCCCTCCTTTTCAAAACCCAACCATTCATTGAACC14390.10458912970796447No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC14160.1029174479961624No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC14090.10240867530126611No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT13950.10139112991147355No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA13930.10124576628436031No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT13820.1004462663352376No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAATA100.0070896083145.010542
TAACACG100.0070896083145.010545
GAATTCG100.00709192144.994748
GAAACAC4700.0115.69994
AACACGT1850.0113.648656
AACACGA3100.0112.2580646
GAGCAGA1700.0110.906531
GACGTCA203.8663688E-4108.756
ACACGAG3200.0108.746057
AAACACG5250.0103.5789645
CACGAGC3450.0100.8659068
ACGAGCA2000.094.246589
ACGTGCC1100.092.269389
ACAACGC2200.088.9740458
ACGAGCC1800.088.6078959
ACGTCAC259.388858E-486.996847
CAACGCA2250.086.996839
CACGTGC2350.086.3798458
TAGAAAC6850.085.736152
TAGTAGA608.2945917E-1084.5894853
Produced by FastQC (version 0.11.5)