FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9497_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9497_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253367
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT8730.34455947301740164No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA7190.2837780768608382No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT6140.24233621584499954No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA5390.2127348865479719No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG5060.19971030165727974No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT4780.18865913871972279No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT4550.17958139773530096No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA4330.1708983411415062No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG4330.1708983411415062No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG4070.16063654698520327No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC4060.16024186259457623No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA4040.1594524938133222No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA3940.15550564990705182No Hit
GTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAA3800.14998006843827333No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG3700.146033224532003No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG3660.14445448696949484No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA3560.14050764306322447No Hit
GTGCTATACTACAAAAATCCCTGAACCAAATTGGGCTGTCTCTGGTTATT3560.14050764306322447No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA3380.13340332403193786No Hit
ATATTAAACATTCCCATCATCATCCCAGGCGACAAAGATGCAGTTCCTTC3340.13182458646942974No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT3300.13024584890692156No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC3250.1282724269537864No Hit
GTATTAAAGTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCA3160.12472026743814309No Hit
ATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAAATCCC3090.12195747670375384No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA3060.12077342353187275No Hit
ACCTAAAAGAGGTTATTGTTGTGTTCAGTGCCGTTGCAGCAGGTTGGTTT3050.12037873914124571No Hit
ATTAGATACAAATCCATCTCTGTAGAACATGCTTGTAAATTCAAACATTC3020.11919468596936461No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT3010.11880000157873756No Hit
CTTTTGATGTATTCCACTTTGGTTATTTTGTCTTTTACCTTCATTGGTAT2890.11406378889121316No Hit
CTTATGGGATGGACTGCAATCTTCTGATGATTTTGCTCTATTTGTTAATG2830.11169568254745094No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT2760.10893289181306168No Hit
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA2750.10853820742243464No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC2740.10814352303180762No Hit
CCCCATAAACATCTTCGAAGCTTATATGTACCCAATCTCACCAAGGTGAG2700.10656478546929948No Hit
CATATGGACCAATAGGAGATACTGAAGGTTTCGAAAGGGTCTACGAAAGC2690.10617010107867243No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC2680.10577541668804541No Hit
CCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAAAAGCTAAA2670.10538073229741837No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC2650.1045913635161643No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT2630.10380199473491022No Hit
TGTTTACTCCCAATAATTTACATGTTCGGTAAAAGTCGTTTATTCCTTCC2590.10222325717240209No Hit
CTAATAGACCATCTCTTCCTTTAGTGTTTTCCCATAACTCCTTTATGATT2580.10182857278177505No Hit
TCATTGGACAAAGCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTT2540.10024983521926692No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAGA151.2279836E-4145.028033
AACACGA800.0126.874486
AAACACG1200.0120.832855
ACACGAG900.0112.777327
TAGAAAC1300.0111.560022
AACACGT404.800313E-9108.749556
CACGAGC950.0106.8416758
ACGAGCC650.0100.38429
GAAACAC1450.099.9995964
ACACGTG451.0883014E-896.6662757
CACGTGC451.0883014E-896.6662758
ACGTGCC406.104365E-790.6246269
ACGAGCA451.2302498E-680.555239
GTAACAA755.4569682E-1177.3330154
GTCAGCT901.3196677E-648.3331389
GCAGAGC803.72246E-545.321263
AGCAGAG855.324086E-542.6553042
TAGATGT2550.042.6468854
GCTGGCC1201.9657091E-742.2914969
GGGAAGA1401.4049874E-841.436581
Produced by FastQC (version 0.11.5)