FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9497_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9497_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1631467
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT55130.3379167338352538No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA45280.27754162358172124No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT37860.23206108367499925No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA34900.2139179033348514No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG32100.19675543544552232No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA29890.18320934471858763No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT28900.17714118642914628No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC28540.17493458341480397No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT28280.17334092568222342No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA28090.1721763296468761No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG27500.16855995248448177No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG27420.16806959625907236No Hit
GTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAA24750.15170395723603358No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA23640.14490026460847813No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG23470.14385825762948315No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG22700.13913857895991766No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA22540.13815786650909886No Hit
GTGCTATACTACAAAAATCCCTGAACCAAATTGGGCTGTCTCTGGTTATT21820.13374466048041425No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA21510.13184453010695282No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC21470.1315993519942481No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT21140.12957663256443433No Hit
GTATTAAAGTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCA20230.1239988305004024No Hit
ACCTAAAAGAGGTTATTGTTGTGTTCAGTGCCGTTGCAGCAGGTTGGTTT20060.12295682352140742No Hit
ATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAAATCCC19990.1225277618241742No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA19470.11934044635901309No Hit
ATATTAAACATTCCCATCATCATCCCAGGCGACAAAGATGCAGTTCCTTC19230.11786937768278488No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT18670.11443688410491908No Hit
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA18660.1143755895767429No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT18490.11333358259774792No Hit
ATTAGATACAAATCCATCTCTGTAGAACATGCTTGTAAATTCAAACATTC18130.1111269795834056No Hit
TGTTTACTCCCAATAATTTACATGTTCGGTAAAAGTCGTTTATTCCTTCC17790.10904296562541564No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATCATTGTCAGGGCC17700.10849131487183007No Hit
CTTTTGATGTATTCCACTTTGGTTATTTTGTCTTTTACCTTCATTGGTAT17350.10634600638566394No Hit
CTTATGGGATGGACTGCAATCTTCTGATGATTTTGCTCTATTTGTTAATG17310.10610082827295923No Hit
CCCCATAAACATCTTCGAAGCTTATATGTACCCAATCTCACCAAGGTGAG17190.10536529393484514No Hit
CCATTATACTTGACATACAGTCCCATCCCCATCCTTTCAGTCTTAGACAT17090.10475234865308339No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC17050.10450717054037868No Hit
CCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAAAAGCTAAA16980.10407810884314546No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC16480.1010133824343367No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT16450.10082949884980819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAGA850.0119.44371
TAGAAAC4650.0116.952412
AATGGCC203.8666298E-4108.74908145
AAACACG5250.0103.586425
GTAACAA4150.099.5927354
GAAACAC5550.097.987164
AACACGA4050.091.306726
ACACGAG4050.091.2955257
ACGAGCC2550.090.979639
AACACGT1950.089.240966
CACGAGC4250.085.2933968
ACACGTG2100.082.8564457
ACGTGCC1450.079.999329
GTATAAA554.38813E-879.112051
CACGTGC2650.065.659828
TAGTAGA606.8016725E-660.4254153
AGAAGCG1450.060.0086865
GCAGAGG851.3045792E-859.7145274
AGCGTTT902.0487278E-856.3953256
GAGCATC400.006048103354.3828744
Produced by FastQC (version 0.11.5)