FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9475_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9475_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52173
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGA1600.30667203342725163No Hit
GTATACGTACACAAATATCTTTGGAGGCGCAATATAGTTTAAAACAACAC1490.28558833112912807No Hit
GTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGTGCTCCAGCTT1490.28558833112912807No Hit
CATATAGGGTGGGTGGAAGAAATGGAGCACTGGCGAACCAATCTGTATGG1470.28175493071128743No Hit
GCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGT1390.2664213290399249No Hit
CATATAGGTTGCCTGAACAGCATTTCCAACTGGCCCAAACGGTGATTGAA1340.25683782799532323No Hit
GTACATTGCTTGGTGATACAGACAAGCCAACTGCTCTGAAAGCACAGAGG1260.24150422632396068No Hit
GGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATG1220.2338374254882794No Hit
GCACTGGTCCGAGCAGCTGCGCGCCTTCAGTGACTATGGCAACCACACTC1210.23192072527935906No Hit
GTATAAAGCATTCGGCCAGCACTGAAAATACCGCTATTACAGCCAGACAT1200.23000402507043877No Hit
GTCCAAGTTTCTGTTACGCTTTCATTTCTAACTCTGTCAGTCCTTGATTA1160.22233722423475744No Hit
GTATATGCTCAACAGCTGGTTTTCCTTCCAAAGACTTAAGCTTTAGCTTG1130.21658712360799648No Hit
GGGTGTACAGTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCG1100.2108370229812355No Hit
AAAGCAGGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTC1090.2089203227723152No Hit
CCCATAAACCTCGAGAATATAAGCATAGTCTCCGCCCGATTTGGAGATTG1060.20317022214555422No Hit
GGTCCGAGCAGCTGCGCGCCTTCAGTGACTATGGCAACCACACTCACGCC1050.2012535219366339No Hit
CTTTAAGGTGATCCTTGCTTTTGGCTTTTTACCTGTACATTGCTTGGTGA1030.19742012151879326No Hit
GTCGTAGGGATGTCAGAAATTATTGCGGTTGGAGCGTATATGAAGTACTG1020.19550342130987292No Hit
CACTCACGCCGTGGCCCTGCTGCGGGAGAAGGTGTACGTGCCCCCCGGGC1010.1935867211009526No Hit
CTTCTATATTGGTGCTATTTTTGTAATTGTCACTGTTTTTCCGTGGGATC1000.19167002089203228No Hit
ATATAGAAGATTAATATACGCCAAATAATACTTTGGATCGCGCTTGTTAA970.18591992026527132No Hit
ATATAAAGGCACACCATTACGAGAAATCTTCGTAAAGAATTTTGGCGCTT970.18591992026527132No Hit
ATATTAATCTTCTATATTGGTGCTATTTTTGTAATTGTCACTGTTTTTCC950.18208651984743068No Hit
CCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGCTGCACTGGTCC950.18208651984743068No Hit
GTACTGGTTTGAGTCTGTGATCAGTCCAAGTTTCTGTTACGCTTTCATTT940.18016981963851036No Hit
ATAATAAATAATATAAAAAGGGAATCAAGCCAGGCAGATATTTTTCCATA930.17825311942959002No Hit
AAATAATACTTTGGATCGCGCTTGTTAACGTTTTTTGCGGATTTTTCGCT910.17441971901174935No Hit
CCTTAAAGAGGAAGAGATGTCAGGGATGAGCTCAGAGGAAAATAGGGATT900.17250301880282903No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT880.1686696183849884No Hit
GTCTTGCTCTGTCCCCCAGGCTGGAGTGCAGGCACACCACCTCGGCTCAC870.16675291817606808No Hit
GCCTGAATCAGTGAATGAATGTGAGCTGTGGAGGTCCAAACACCCCAAAC870.16675291817606808No Hit
GTAAGAAGGGGAAGAGGCTGACGTACCCCAGTGCGTTGACAAACTGCAGC860.16483621796714776No Hit
CTATAGAGTAGAGGGCCCTCCGGACCTAGCTCTCGTACGAGTTTCTGAGC850.16291951775822744No Hit
CCCCAAACCACCTCATCCCTTGAACGAATCCGCGTTTCACAACGAGGCTC820.15716941713146648No Hit
GTATTATTTGGCGTATATTAATCTTCTATATTGGTGCTATTTTTGTAATT810.15525271692254616No Hit
GTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGTGCT780.14950261629578518No Hit
GTACGTATACAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCA780.14950261629578518No Hit
GACAAAGATGTGAGCAATGTTGTCGGAGAAGAAACTGTTGCTTTACAAAG780.14950261629578518No Hit
TCTTTAAGGTGATCCTTGCTTTTGGCTTTTTACCTGTACATTGCTTGGTG780.14950261629578518No Hit
GTATGGATGATGGGCCTATCTACCTCGTTCTCGGCGAACCAACCTAACTG770.14758591608686486No Hit
TCTTAATACTCTCTAACCCATTCGTCATCATATCTTTTCCGGCTGCTATG760.1456692158779445No Hit
CCTTCAGTGACTATGGCAACCACACTCACGCCGTGGCCCTGCTGCGGGAG750.1437525156690242No Hit
TCTCAAAGGGCCCCGGGTCTCTCCAGCTGCCCCGCCAACCTCACCCTCCC720.13800241504226324No Hit
AAGCAGGAGTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCT720.13800241504226324No Hit
CTCATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAACACCTGCAG710.13608571483334292No Hit
CACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGCTGCACTGGTCCGA680.13033561420658196No Hit
TACGTATACAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCAT680.13033561420658196No Hit
GTATACTGCCGGATCGGTCGAAATCAGGCTGCGGCTATGTGCTGGAAAAG670.12841891399766162No Hit
TCACACCACTGCACTCCAGCCTAGATGACAGAGTGAGACTCGGTCTCAAA670.12841891399766162No Hit
GTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGC660.1265022137887413No Hit
GAGCAAGACTTCATCTCAAATAATAATAATAAATAATATAAAAAGGGAAT660.1265022137887413No Hit
ATCTAACCTTTGGGCACATGGCGGCTTTTTTACAGGTGGCTGGTCAGGAT660.1265022137887413No Hit
AATTAATACCATCATTAAGAAGGCAATCGTCAAATAGTTTGTAACAGGAG660.1265022137887413No Hit
CTGTTACACTGACTATCTGTCTGAAGTAGTTTGACTGCTTTTATTGTTGT650.12458551357982098No Hit
CTCATGTCCCTCAGGATGTGCTCCAGCTTGGGCGAGTCGGGCGTCAGGAT650.12458551357982098No Hit
GCTTAGGGGCATTACGCCCTGAGCCGACCGAGCGCACCAGGCGTGCCACG650.12458551357982098No Hit
CCCTCAGGATGTGCTCCAGCTTGGGCGAGTCGGGCGTCAGGATGTGCAGT640.12266881337090066No Hit
GACTATGGCAACCACACTCACGCCGTGGCCCTGCTGCGGGAGAAGGTGTA640.12266881337090066No Hit
GCTTTATACATTGGGAGTGAATGGTCAAGCGCCAAAATTCTTTACGAAGA630.12075211316198034No Hit
GTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCGTGTCCTCGC620.11883541295306002No Hit
ATGTGAAACAAGCCATCAACTATCATTGCTTGGCTTGTCATGAGGAAGAG620.11883541295306002No Hit
ACTCTATAGTTGGCATATCTGGTTCGCCGGCATGCATGGAAGAACCATTC610.1169187127441397No Hit
ATCCAGCACCATTCAATGGACGGGTCCGTCTGTACTGCTTGCTTATGCAA610.1169187127441397No Hit
GAAGTAAGGAGTTTGAGACCAGCCTGGACAAAATGGTGAAACCCCACTTC610.1169187127441397No Hit
AGCGAAAGCAGGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTC600.11500201253521938No Hit
TCCCTGACATCTCTTCCTCTTTAAGGTGATCCTTGCTTTTGGCTTTTTAC600.11500201253521938No Hit
ACGTACCCCAGTGCGTTGACAAACTGCAGCTTAGGGGCATTACGCCCTGA590.11308531232629904No Hit
GGAGGGGGAAGAGCGAGCTTCCTATGGAATGGTTTCAGTTCCCAATTCGG590.11308531232629904No Hit
GTTCTACAATAATTTGGCGTTTTGCCAGTTCCTCACGGTCAAGAGCCGTA590.11308531232629904No Hit
CCTCTATGACTCTTTAGGAGGCATCTCGCTTGAGCTTACCCTTTGCAAAA590.11308531232629904No Hit
AGCAGGGAGAGACCCATAAACCTCGAGAATATAAGCATAGTCTCCGCCCG590.11308531232629904No Hit
GTGCAGTAAGAAGGGGAAGAGGCTGACGTACCCCAGTGCGTTGACAAACT580.11116861211737872No Hit
ATATAAAAAGGGAATCAAGCCAGGCAGATATTTTTCCATAGGAGGGGGAA580.11116861211737872No Hit
GAATGAATGTGAGCTGTGGAGGTCCAAACACCCCAAACCACCTCATCCCT580.11116861211737872No Hit
CCCTATGGCCTGTTGCAGCATGGACTAGCGCTTTTTCTTCTGATTGAGTT580.11116861211737872No Hit
AGCATATACTGATCCCCGAGAGCCTCACATCAAATTTGCTAAGAAGGACC570.10925191190845839No Hit
GTGATACAGACAAGCCAACTGCTCTGAAAGCACAGAGGTAATTCCAGAGC570.10925191190845839No Hit
GGGTAAGCTCAAGCGAGATGCCTCCTAAAGAGTCATAGAGGTGTGCTCTG570.10925191190845839No Hit
GGAATTACCTCTGTGCTTTCAGAGCAGTTGGCTTGTCTGTATCACCAAGC570.10925191190845839No Hit
GTATTAAGAAAAACCAGGCATAACCCGCCCGGTCCTTTGTCATGATGAGA570.10925191190845839No Hit
ACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGCTGCACTGGTCCGAG570.10925191190845839No Hit
GCTCCAGCTTGGGCGAGTCGGGCGTCAGGATGTGCAGTAAGAAGGGGAAG560.10733521169953807No Hit
GATAAGGAATTTAGAGACAGGCTCCCAGCTCTGGGTGAATGACAGTAAAT560.10733521169953807No Hit
CCCCAGTGCGTTGACAAACTGCAGCTTAGGGGCATTACGCCCTGAGCCGA560.10733521169953807No Hit
CTTAAGAAGACCGTTAAAAAAATAGGGGAACAATCCGTGCCTATCATTGC560.10733521169953807No Hit
CCCTCCCACTCATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAAC560.10733521169953807No Hit
CTCTAACCCATTCGTCATCATATCTTTTCCGGCTGCTATGGCACCACTCC560.10733521169953807No Hit
GGAGTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCG550.10541851149061775No Hit
GTCCTTGATTATTTGGCAAGACGAGGCTTGCTTCAACCTCCTGACATGAA550.10541851149061775No Hit
ATACAATTGTTACAATTTTAATCATCGCAAACCAAAATTCAAATTCACCA540.10350181128169743No Hit
CATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAACACCTGCAGGT540.10350181128169743No Hit
ATGTACAGGTAAAAAGCCAAAAGCAAGGATCACCTTAAAGAGGAAGAGAT540.10350181128169743No Hit
ACATTACGGAGATGCTTACCACACTTTATCCTAAACAAATATTTGAGGAA530.10158511107277711No Hit
GGTTTGAGTCTGTGATCAGTCCAAGTTTCTGTTACGCTTTCATTTCTAAC530.10158511107277711No Hit
GCCTAATAGTATCAGTATCTATTACCAAGTGATGAGAGTAAGGCAAGCTC530.10158511107277711No Hit
CTCAGGATGTGCTCCAGCTTGGGCGAGTCGGGCGTCAGGATGTGCAGTAA530.10158511107277711No Hit
AAAGCAGGCAGATCACCTGAAGTAAGGAGTTTGAGACCAGCCTGGACAAA530.10158511107277711No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGA100.007051608145.13821
CAAGATT100.007071879144.999044
CAAAGTA100.007071879144.999044
AAAGTAT100.007071879144.999045
CCAAGAT100.007071879144.999043
CGAAAGC501.8189894E-12115.999243
CACAGCA256.407343E-6115.999242
GACGAGA203.845418E-4108.74928145
GCGAAAG553.6379788E-12105.453852
GCACAGC301.5777197E-596.75881
ACAGCAC301.5852671E-596.666033
CCTAATA353.4068653E-582.856592
GTATCAG353.4068653E-582.856599
TAGTATC353.4068653E-582.856597
AATAGTA353.4068653E-582.856595
CTAATAG353.4068653E-582.856593
TAATAGT353.4068653E-582.856594
AAGATTC300.001925484572.499535
ATAGTAT406.604405E-572.499526
GTATACG451.17774216E-464.505861
Produced by FastQC (version 0.11.5)