FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9475_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9475_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347789
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGA11120.3197340916475219No Hit
GTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGTGCTCCAGCTT9770.2809174528234073No Hit
CATATAGGGTGGGTGGAAGAAATGGAGCACTGGCGAACCAATCTGTATGG9300.26740351189945627No Hit
GTATACGTACACAAATATCTTTGGAGGCGCAATATAGTTTAAAACAACAC9180.2639531440039794No Hit
GGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATG8820.25360204031754885No Hit
CATATAGGTTGCCTGAACAGCATTTCCAACTGGCCCAAACGGTGATTGAA8550.24583871255272594No Hit
GCACTGGTCCGAGCAGCTGCGCGCCTTCAGTGACTATGGCAACCACACTC8100.23289983294468775No Hit
GTATAAAGCATTCGGCCAGCACTGAAAATACCGCTATTACAGCCAGACAT7970.22916193439125446No Hit
GCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGT7950.22858687307534165No Hit
GTACATTGCTTGGTGATACAGACAAGCCAACTGCTCTGAAAGCACAGAGG7820.2248489745219084No Hit
GGTCCGAGCAGCTGCGCGCCTTCAGTGACTATGGCAACCACACTCACGCC7680.22082354531051873No Hit
GTATATGCTCAACAGCTGGTTTTCCTTCCAAAGACTTAAGCTTTAGCTTG7530.2165105854411727No Hit
CCCATAAACCTCGAGAATATAAGCATAGTCTCCGCCCGATTTGGAGATTG7080.20357170583313447No Hit
GTCCAAGTTTCTGTTACGCTTTCATTTCTAACTCTGTCAGTCCTTGATTA7070.20328417517517808No Hit
GGGTGTACAGTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCG6990.2009839299115268No Hit
AAAGCAGGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTC6980.20069639925357044No Hit
CCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGCTGCACTGGTCC6850.19695850070013715No Hit
GTCGTAGGGATGTCAGAAATTATTGCGGTTGGAGCGTATATGAAGTACTG6840.19667097004218073No Hit
ATATAAAGGCACACCATTACGAGAAATCTTCGTAAAGAATTTTGGCGCTT6630.19063282622509625No Hit
CTTCTATATTGGTGCTATTTTTGTAATTGTCACTGTTTTTCCGTGGGATC6590.18948270359327063No Hit
CACTCACGCCGTGGCCCTGCTGCGGGAGAAGGTGTACGTGCCCCCCGGGC6440.18516974372392456No Hit
GTACTGGTTTGAGTCTGTGATCAGTCCAAGTTTCTGTTACGCTTTCATTT6140.1765438239852324No Hit
ATATTAATCTTCTATATTGGTGCTATTTTTGTAATTGTCACTGTTTTTCC6130.17625629332727602No Hit
CTTTAAGGTGATCCTTGCTTTTGGCTTTTTACCTGTACATTGCTTGGTGA6130.17625629332727602No Hit
GTATGGATGATGGGCCTATCTACCTCGTTCTCGGCGAACCAACCTAACTG5740.16504259766697624No Hit
AAATAATACTTTGGATCGCGCTTGTTAACGTTTTTTGCGGATTTTTCGCT5710.16418000569310703No Hit
ATATAGAAGATTAATATACGCCAAATAATACTTTGGATCGCGCTTGTTAA5700.16389247503515061No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT5630.1618797604294558No Hit
ATAATAAATAATATAAAAAGGGAATCAAGCCAGGCAGATATTTTTCCATA5530.15900445384989173No Hit
TCTTTAAGGTGATCCTTGCTTTTGGCTTTTTACCTGTACATTGCTTGGTG5510.15842939253397895No Hit
GCCTGAATCAGTGAATGAATGTGAGCTGTGGAGGTCCAAACACCCCAAAC5420.15584161661237128No Hit
GTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGC5370.1544039633225893No Hit
CTATAGAGTAGAGGGCCCTCCGGACCTAGCTCTCGTACGAGTTTCTGAGC5340.15354137134872006No Hit
GACAAAGATGTGAGCAATGTTGTCGGAGAAGAAACTGTTGCTTTACAAAG5160.1483658195055048No Hit
TACGTATACAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCAT5130.14750322753163556No Hit
CCCCAAACCACCTCATCCCTTGAACGAATCCGCGTTTCACAACGAGGCTC5080.14606557424185354No Hit
GTAAGAAGGGGAAGAGGCTGACGTACCCCAGTGCGTTGACAAACTGCAGC5060.14549051292594073No Hit
CCTTAAAGAGGAAGAGATGTCAGGGATGAGCTCAGAGGAAAATAGGGATT5020.1443403902941151No Hit
GTATTATTTGGCGTATATTAATCTTCTATATTGGTGCTATTTTTGTAATT5020.1443403902941151No Hit
CCTTCAGTGACTATGGCAACCACACTCACGCCGTGGCCCTGCTGCGGGAG4920.14146508371455105No Hit
GTCTTGCTCTGTCCCCCAGGCTGGAGTGCAGGCACACCACCTCGGCTCAC4880.14031496108272545No Hit
AAGCAGGAGTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCT4720.13571447055542296No Hit
GTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTCCCTCAGGATGTGCT4650.13370175594972813No Hit
ATGTGAAACAAGCCATCAACTATCATTGCTTGGCTTGTCATGAGGAAGAG4550.1308264493701641No Hit
CTCTAACCCATTCGTCATCATATCTTTTCCGGCTGCTATGGCACCACTCC4550.1308264493701641No Hit
CACCAGATGCTCAGCGACAACCAGCTGCTGGCCGAGCTGCACTGGTCCGA4540.13053891871220769No Hit
GGAGGGGGAAGAGCGAGCTTCCTATGGAATGGTTTCAGTTCCCAATTCGG4380.12593842818490522No Hit
CTCATGTCCCTCAGGATGTGCTCCAGCTTGGGCGAGTCGGGCGTCAGGAT4330.1245007748951232No Hit
AGCGAAAGCAGGCCGTATGGAGTCCACAGGCGGTCGGGGTCCCTCATGTC4320.12421324423716679No Hit
AATGTGAGCTGTGGAGGTCCAAACACCCCAAACCACCTCATCCCTTGAAC4280.12306312160534118No Hit
ACTCTATAGTTGGCATATCTGGTTCGCCGGCATGCATGGAAGAACCATTC4260.12248806028942835No Hit
TCTTAATACTCTCTAACCCATTCGTCATCATATCTTTTCCGGCTGCTATG4250.12220052963147195No Hit
ATCCAGCACCATTCAATGGACGGGTCCGTCTGTACTGCTTGCTTATGCAA4230.12162546831555915No Hit
GAGCAAGACTTCATCTCAAATAATAATAATAAATAATATAAAAAGGGAAT4130.1187501617359951No Hit
GTACGTATACAGTGCAAGTGTGCTGCCGGGTATGATCCCATGGTTTGTCA4100.1178875697621259No Hit
AGCGAAAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCA4070.11702497778825667No Hit
GTCCTTGATTATTTGGCAAGACGAGGCTTGCTTCAACCTCCTGACATGAA4060.11673744713030026No Hit
GTATTAAGAAAAACCAGGCATAACCCGCCCGGTCCTTTGTCATGATGAGA4050.11644991647234387No Hit
GGTTTGAGTCTGTGATCAGTCCAAGTTTCTGTTACGCTTTCATTTCTAAC4030.11587485515643105No Hit
TCTCAAAGGGCCCCGGGTCTCTCCAGCTGCCCCGCCAACCTCACCCTCCC4020.11558732449847464No Hit
GTATACTGCCGGATCGGTCGAAATCAGGCTGCGGCTATGTGCTGGAAAAG4020.11558732449847464No Hit
CTCATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAACACCTGCAG4010.11529979384051825No Hit
GCTTTATACATTGGGAGTGAATGGTCAAGCGCCAAAATTCTTTACGAAGA4000.11501226318256184No Hit
CCTATAGATAGTAGGGCGAATCATTGACTTTTTATAACTACTCATGCTAA4000.11501226318256184No Hit
GCAATGTACAGGTAAAAAGCCAAAAGCAAGGATCACCTTAAAGAGGAAGA3990.11472473252460544No Hit
CTGTTACACTGACTATCTGTCTGAAGTAGTTTGACTGCTTTTATTGTTGT3990.11472473252460544No Hit
GATAAGGAATTTAGAGACAGGCTCCCAGCTCTGGGTGAATGACAGTAAAT3960.11386214055073623No Hit
GAAGTAAGGAGTTTGAGACCAGCCTGGACAAAATGGTGAAACCCCACTTC3960.11386214055073623No Hit
GTGATACAGACAAGCCAACTGCTCTGAAAGCACAGAGGTAATTCCAGAGC3950.11357460989277982No Hit
AGCATATACTGATCCCCGAGAGCCTCACATCAAATTTGCTAAGAAGGACC3950.11357460989277982No Hit
GTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCGTGTCCTCGC3940.11328707923482341No Hit
ATCTAACCTTTGGGCACATGGCGGCTTTTTTACAGGTGGCTGGTCAGGAT3870.11127436462912857No Hit
TCCCTGACATCTCTTCCTCTTTAAGGTGATCCTTGCTTTTGGCTTTTTAC3870.11127436462912857No Hit
GACTATGGCAACCACACTCACGCCGTGGCCCTGCTGCGGGAGAAGGTGTA3840.11041177265525937No Hit
GTGCAGTAAGAAGGGGAAGAGGCTGACGTACCCCAGTGCGTTGACAAACT3810.10954918068139016No Hit
CTTAAGAAGACCGTTAAAAAAATAGGGGAACAATCCGTGCCTATCATTGC3780.10868658870752093No Hit
GGTAATTCCAGAGCATCCACCTTTCTTCATTTACTGTCATTCACCCAGAG3780.10868658870752093No Hit
TATAAGCATAGTCTCCGCCCGATTTGGAGATTGTGGTGCCAAGTTCAGCG3740.10753646607569531No Hit
GTTCTACAATAATTTGGCGTTTTGCCAGTTCCTCACGGTCAAGAGCCGTA3740.10753646607569531No Hit
AAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCACTCTG3710.1066738741018261No Hit
CCCTATGGCCTGTTGCAGCATGGACTAGCGCTTTTTCTTCTGATTGAGTT3710.1066738741018261No Hit
TTCAGTGACTATGGCAACCACACTCACGCCGTGGCCCTGCTGCGGGAGAA3700.1063863434438697No Hit
GGGTTAGAGAGTATTAAGAAAAACCAGGCATAACCCGCCCGGTCCTTTGT3680.1058112821279569No Hit
GATTAATATACGCCAAATAATACTTTGGATCGCGCTTGTTAACGTTTTTT3680.1058112821279569No Hit
CCCTCAGGATGTGCTCCAGCTTGGGCGAGTCGGGCGTCAGGATGTGCAGT3660.10523622081204409No Hit
ACGTACCCCAGTGCGTTGACAAACTGCAGCTTAGGGGCATTACGCCCTGA3640.10466115949613126No Hit
GGAGTACGAGCGCACCCACCAGATGCTCAGCGACAACCAGCTGCTGGCCG3630.10437362883817487No Hit
AATTAATACCATCATTAAGAAGGCAATCGTCAAATAGTTTGTAACAGGAG3630.10437362883817487No Hit
GCTTAGGGGCATTACGCCCTGAGCCGACCGAGCGCACCAGGCGTGCCACG3630.10437362883817487No Hit
CATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAACACCTGCAGGT3620.10408609818021847No Hit
AGCAGGGAGAGACCCATAAACCTCGAGAATATAAGCATAGTCTCCGCCCG3600.10351103686430566No Hit
ATCTATAGGCGAGTTATTTCACGCCTCCCATCTCCTCCGGAACTCTTTCT3590.10322350620634924No Hit
CCCTCCCACTCATTAATCGGCCTTCAGCTCCCATTTTGTCAGCGGCAAAC3580.10293597554839286No Hit
ATATAAAAAGGGAATCAAGCCAGGCAGATATTTTTCCATAGGAGGGGGAA3580.10293597554839286No Hit
GTAGTAAACAGAGCACACCTCTATGACTCTTTAGGAGGCATCTCGCTTGA3570.10264844489043645No Hit
TCACACCACTGCACTCCAGCCTAGATGACAGAGTGAGACTCGGTCTCAAA3560.10236091423248003No Hit
GAATGAATGTGAGCTGTGGAGGTCCAAACACCCCAAACCACCTCATCCCT3540.10178585291656723No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCATGG100.007083089145.039692
GGTGTCG100.0070891827144.997998
ACAAGGC100.0070891827144.997997
GAGCGAA256.4408923E-6116.065141
GCGAAAG3150.0101.297572
GTAGAAA301.5935359E-596.720951
CGAAAGC3300.096.693143
CTAACAG353.429458E-582.879832
AGATTCT651.5606929E-978.075848
TAAAGAT702.7994247E-972.519855
AAAGATT702.7994247E-972.519856
TAGAAAC406.648059E-572.5198442
AGGCCGT2000.072.5198446
GCAGGCC2150.070.833344
GGACTAA754.820322E-967.7046661
TATAGGG2900.067.518483
ATAGGGT2950.066.37414
GAGTACG3350.064.961841
AGTACGA3350.064.9431462
ACTAAAG808.049028E-963.4548653
Produced by FastQC (version 0.11.5)