FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9420_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9420_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2631827
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG165830.6300946072823176No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC132960.5052003798121989No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA115740.4397705472282183No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAG107820.4096773838098021No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT104400.3966826086973042No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG102450.38927330709807295No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC90380.34341163001975433No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG86110.3271871593383608No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT84910.32262758912344924No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG76510.2907105976190684No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA74820.2842892028997347No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC70530.2679887393814259No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG69340.26346716558497196No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC66730.2535501003675394No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA65300.2481166125281031No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT62960.23922545060902559No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC61320.2329940379819798No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT60350.22930838539159298No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAGTGCATGTGTC57700.21923933450033No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT52460.19932921122854957No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG51030.19389572338911334No Hit
TAGTAGCACTGCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAA50590.19222388097697912No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC48380.18382667249785034No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC47900.18200284441188574No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA47460.1803310019997515No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT47130.17907712019065083No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC46230.1756574425294672No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA46060.17501150341568805No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG45260.17197178993908033No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC45150.1715538293360468No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT44620.16954001915779418No Hit
ATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA44560.1693120406470486No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC43910.16684227344730485No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC43860.16665229135501688No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA43590.16562638805666177No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA43320.1646004847583067No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC41810.15886302557120965No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG40620.15434145177475572No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG40590.1542274625193829No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA40410.15354352698714618No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA39920.15168170248272397No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT39870.15149172039043599No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGA39610.1505038135105385No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA39150.14875597826148906No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG38880.14773007496313398No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA38300.14552628269259338No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC37850.14381644386200157No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG37290.1416886444283762No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTT36840.13997880559778436No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT36490.13864893095176847No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTTAAC35550.13507726761675443No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA35460.13473529985063606No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA35090.13332943236770503No Hit
GTTAAAGCACAGATTTGAAATAATCGAGGGGAGAGACAGAACAATGGCCT34530.13120163293407963No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC33950.12899784066353906No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT33950.12899784066353906No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC33680.12797193736518397No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT33620.12774395885443837No Hit
CACTTGGAGGTGTGTTTCATGTATTCAAGTCAGTATTCAATAGCCTGTAT33240.12630009495304972No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT32910.12504621314394906No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT32770.1245142632855427No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT32740.12440027403016991No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA32630.12398231342713635No Hit
GTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAACTTCTGACCTA32470.12337437073181481No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA32030.12170252831968059No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG31970.121474549808935No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT31790.12079061427669828No Hit
CTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGT31780.1207526178582407No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG31580.11999268948908876No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA31360.11915676828302163No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA30480.11581308345875317No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT30390.11547111569263481No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA30240.11490116941577086No Hit
CTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCATTGAGCA30070.11425523030199174No Hit
CCCTAGAAGATCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA30030.11410324462816136No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT29610.1125073950529423No Hit
ATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCAGAGT29540.11224142012373912No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC29410.11174746668379039No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC29270.11121551682538404No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT29200.11094954189618086No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGTCATTTGAATCGTTGCA28980.11011362069011374No Hit
GTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG28340.10768184990882759No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCATT28290.1074918678165396No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT28260.10737787856116682No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA27960.10623798600743893No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG27930.10612399675206616No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC27920.10608600033360856No Hit
GTATGGGGAGGATCTGAAAATTGAAACCAACAAATTTGCAGCAATATGCA27800.1056300433121174No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT27800.1056300433121174No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCG27800.1056300433121174No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA27790.10559204689365981No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG27680.10517408629062625No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGA27540.1046421364322199No Hit
TCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCT27490.10445215433993191No Hit
GTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAAT27260.1035782367154072No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA27000.1025903298355097No Hit
GCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGT26920.10228635848784894No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC26780.10175440862944259No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC26710.10148843370023941No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG26440.10046253040188431No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCGA26410.10034854114651154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTATA256.464128E-6116.009832
AGCGAAA6400.0114.456371
TATATAT301.5992742E-596.674855
CGAAAGC7850.093.288153
CGACTCC259.388848E-486.9991145
GCTGTCC259.388848E-486.9991145
GTACTGA14850.083.0020756
GATAATT1400.082.864162
TACTGAT14900.082.717267
AGTAGAA15500.078.1417851
GAGCGAA1350.075.212991
TTATATT8500.075.065182
TATATTC8550.074.6262053
TAAACTA5000.071.056015
TTTAAAC5100.069.6627663
AATTCTA1700.068.241075
CAGTACG19450.066.721679
CAGCATG9850.064.77099
ATATTCA10150.062.8624654
ACTGATT19950.061.778818
Produced by FastQC (version 0.11.5)