FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9376_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9376_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345849
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG66270.4924029367336157No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC57400.4264965831976693No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG47870.35568626198035586No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG43580.32381047205147084No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT43060.31994673993887873No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG34810.2586471439217921No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA34370.25537783213421417No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT32240.2395513909807118No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAG29530.2194154024708567No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC29110.21629469576453225No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG28240.20983037473000316No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC27230.2023258181266992No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA27150.20173139780168503No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA26570.19742185044533228No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC25640.19051171416704252No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT25590.19014020146390864No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT24370.18107529150744253No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG23510.17468527301354014No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA23000.1708958434415748No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT22790.1693354900884126No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA21950.16309407667576378No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT21620.1606420928350803No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC20980.1558867302349669No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC20960.15573812515371338No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC20600.1530632336911496No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA20440.15187439304112127No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC18550.13783121286266142No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA18440.1370138849157669No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG18250.13560213664385826No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT18220.13537922902197796No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA18030.13396748075006928No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG17940.13329875788442833No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT17490.12995514355622362No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA17450.1296579333937165No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC17440.12958363085308974No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG17070.1268344368498992No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA17060.12676013430927244No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA16960.12601710890300472No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC16860.125274083496737No Hit
GTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAGTGCATGTGTC16550.12297070473730708No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG16370.12163325900602519No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC16310.12118744376226455No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT15980.11873545992158109No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC15860.11784382943405983No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA15820.11754661927155276No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA15530.11539184559337637No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT15520.1153175430527496No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC15470.11494603034961574No Hit
CCTTAGTAACATATCTCCTATCTCAAGGACACAATATTTCTCCCATTTAT15450.1147974252683622No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT15260.11338567699645354No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT15220.11308846683394645No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA15120.11234544142767874No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTT14740.10952194488386142No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG14680.10907612964010079No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC14630.10870461693696692No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT14580.10833310423383306No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG14580.10833310423383306No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG14550.10811019661195276No Hit
CTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGT14440.10729286866505827No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA14430.1072185661244315No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC14410.10706996104317794No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA14360.10669844834004409No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC14210.10558391023064251No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC14180.1053610026087622No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA14150.10513809498688188No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT14110.1048408848243748No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTTTTAAC14030.10424646449936062No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT14010.10409785941810708No Hit
GTAGTAAACAGTATCTATGGCCCATTGGGGAGTCCCCCAAAGGAGTGGAA13780.10238890098369134No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT13680.10164587557742362No Hit
GCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGT13670.10157157303679686No Hit
CTATAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGTCCT13630.10127436287428976No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA13610.10112575779303622No Hit
TAGTAGCACTGCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAA13560.10075424508990236No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT13490.10023412730551495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA550.0145.087541
AGTAAAG203.8622136E-4108.779272
CGAAAGC3750.085.0895543
CAGTACG5600.071.2033549
TATATTC3050.068.952973
TTATATT3100.067.8408362
AGTAGAA7550.067.2591251
TACTGAT2500.066.698127
TAAACTA2850.066.1556855
TTCATAA554.061254E-665.91213145
TTTAAAC2950.063.9155043
GTTGATA2400.063.4758031
GTACTGA2650.062.934456
ATATAAG3000.062.871271
GTCCAAC702.3325993E-762.1526536
TGATAAT2500.060.916393
ATATTCA3600.060.430684
CAGCATG3600.058.401139
CTATTCA3000.057.9983639
CCAACCC753.7629252E-757.9983638
Produced by FastQC (version 0.11.5)