FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_9350_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9350_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944188
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCG90180.955106398302033No Hit
GGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGA85860.9093527983833729No Hit
AGCGAAAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTG58330.6177795100128365No Hit
AGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAG50070.5302969323905832No Hit
CCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGT46150.4887797769088359No Hit
ACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCC43830.46420839917474066No Hit
AAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTC43530.4610310658470559No Hit
GCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGT40130.42502128813329554No Hit
GCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGG39460.4179252437014662No Hit
AAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGG38930.41231195482255656No Hit
GTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTG38640.40924053260579457No Hit
CTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGA36680.388481954864921No Hit
AGCGAAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGT34650.3669819993475876No Hit
GTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTG32450.34368155494456615No Hit
GCCGTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGA31510.3337259105178206No Hit
GATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCA31350.3320313327430554No Hit
TTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTG29310.31042546611479915No Hit
GCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAA29270.31000182167110785No Hit
GTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGC29010.30724813278711444No Hit
CTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAG28280.29951662168974824No Hit
CCCCAACCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTGGGTGCATT27900.2954919994746809No Hit
GGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTG27110.28712502171177773No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA26780.28362995505132454No Hit
CCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGC26510.28077035505640824No Hit
GAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCT26130.27674573284134096No Hit
AAGCAGCACTGGTCAGAAATCATTAATAACTGCCCCTCATTTGAAGCACT22910.24264235512419136No Hit
GACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTC22270.2358640440251306No Hit
AAGCAGGGCCATGTGGGTAAGAAGGAGTGGAGATTTATGACAAAAAAGAG21620.228979821815147No Hit
CCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGA21110.22357835515808291No Hit
GCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTAGTGG20800.22029511071947533No Hit
GCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGTCGGACCT20700.2192359996102471No Hit
TTGTAAAAGTGAGTGTAATTTCATTCAAGTCGCAAACATAAGCACCCTTG20360.21563502183887107No Hit
AAGCAGGGGTGCTTATGTTTGTCGAGGTTTGTAAAAGTGAGTGTAATTTC20200.2139404440641059No Hit
CTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTAGT19870.21044537740365268No Hit
CATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGACAG19790.2095980885162701No Hit
GAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCGAGG19550.20705622185412226No Hit
GTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAG19390.2053616440793571No Hit
GTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTA19010.20133702186428973No Hit
CTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCC18940.20059564408782998No Hit
CACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCT18690.19794786631475936No Hit
ATCTTATAGCGAAGACTACATGGAAATAGCTGTCTTGATTATTAACAGCG18480.19572373298538No Hit
CCTATAGTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTTGT18470.19561782187445723No Hit
GCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTG18200.19275822187954095No Hit
GTCCAATACAAGGCTGACCACTGGGGTAATTACCAAAGCAGCCTCTCTTC18150.1922286663249268No Hit
GCGAAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTC18050.19116955521569856No Hit
GGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGC17920.18979271077370186No Hit
GTGCTTATGTTTGTCGAGGTTTGTAAAAGTGAGTGTAATTTCATTCAAGT17770.18820404410985947No Hit
GTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGG17380.1840735107838693No Hit
CCCTTGGTGAGCACCCCCAAGTGTAAGGTTTACTCAAAGAGCCTCTCCTC17340.18364986634017802No Hit
CTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGA16620.17602426635373464No Hit
GTATTAATTAGCATAATTGCAGTGACCGGGCTGCAATTGCTGCGCGGATG16570.17549471079912052No Hit
GGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGC16520.1749651552445064No Hit
GGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACA16230.17189373302774447No Hit
TGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCA16220.17178782191682165No Hit
GAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCG15980.16924595525467384No Hit
GGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCCCTGTC15720.16649226637068043No Hit
ATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGTCG15690.16617453303791194No Hit
TCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTT15660.16585679970514347No Hit
CTGCGGAGGCAGCCCTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCA15500.16416222193037827No Hit
GGGTAAGAAGGAGTGGAGATTTATGACAAAAAAGAGATATTACATGCCTC15370.16278537748838157No Hit
CACTAAGGCCTACTGTAGCTAGACGCACTCAGCAGGATGCAGTGTTACAG15280.1618321774900761No Hit
GTGGATATGAGGAGCACAGTGAGGAGCCCATCCAAGGACCACTACAGCCT15000.15886666638423705No Hit
GTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAG14830.157066177498549No Hit
CCGTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAG14810.15685435527670336No Hit
CCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGACG14520.15378293305994145No Hit
GTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGCT14420.1527238219507132No Hit
GATTGAGGCTCAATCTCTGCGGGGTTGCTGCCATTCTTAACCCCCCTCCG14340.15187653306333063No Hit
GAAATGGATTCATGCCTGGAACAGCAGATGAGAAGAAACTCAAGTCGATA14290.1513469775087165No Hit
TCCCAGCGCCCCAACCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTG14250.15092333306502517No Hit
GGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAA14240.15081742195410236No Hit
GAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGC14090.14922875529026No Hit
TCTTAACCCCCCTCCGATCAGAAATGGATTCATGCCTGGAACAGCAGATG14070.14901693306841435No Hit
CCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTG13970.1479578219591861No Hit
TCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGA13880.14700462196088065No Hit
ATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAG13840.14658097751718938No Hit
GCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCGAGGTCGAG13830.14647506640626654No Hit
GTGTAAGGTTTACTCAAAGAGCCTCTCCTCTGAAGTGGAGGCCTGCTGTG13780.14594551085165242No Hit
GCGAAAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGC13570.1437213775222731No Hit
GGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCC13500.14297999974581332No Hit
GTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGT13290.14075586641643403No Hit
GGTCAGAAATCATTAATAACTGCCCCTCATTTGAAGCACTCAGGGAGTGT13270.14054404419458838No Hit
GATTAAAGTGGGAGGTGTTGCTTTGTGGAACAGAACACATCTTTTTTTCT13190.13969675530720577No Hit
GCCTTGCGTTTCCGTGGAGACAGGGGTTGGGGCGCTGGGATGCGGCAGCC13140.13916719975259165No Hit
CATAAGCACCCTTGGTGAGCACCCCCAAGTGTAAGGTTTACTCAAAGAGC13130.1390612886416688No Hit
GACGAGAGGAGGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCC13010.13779035531059491No Hit
ATCTAAGACAGCGGAAGGGCACGGGAGGTTGGCCAAAAGTGTTTTGGTGT12970.13736671086690364No Hit
CCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTG12970.13736671086690364No Hit
GCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGA12810.13567213309213844No Hit
GTAAAAGTGAGTGTAATTTCATTCAAGTCGCAAACATAAGCACCCTTGGT12630.13376573309552758No Hit
GTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCG12620.13365982198460477No Hit
CTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATA12420.13154159976614826No Hit
TCTTTATTCAGTGCATTTTTTTATTTCCATTTCTCTCTGCTGCTCTTAGA12400.1313297775443026No Hit
GCGTTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGC12310.13037657754599719No Hit
GAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGT12280.13005884421322872No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT12240.12963519976953744No Hit
GTGTGAGGGAGAGAGATTGAGTGAGAGAGAGAAAGTTTTGTTCCTCTTAA12160.12878791088215483No Hit
GTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTG12140.12857608866030917No Hit
CCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCG11970.12677559977462116No Hit
AGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGCTGCCG11850.12550466644354727No Hit
GACCTATAGTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTT11770.12465737755616466No Hit
CTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAG11740.12433964422339618No Hit
CTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCCCTGTCTCC11610.12296279978139947No Hit
GGGTTGGGGCGCTGGGATGCGGCAGCCGTGGCGACCGTGGAGACCTCCTC11400.12073866645202014No Hit
GTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATC11320.11989137756463755No Hit
CACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCG11220.11883226645540931No Hit
GATATGAGGAGCACAGTGAGGAGCCCATCCAAGGACCACTACAGCCTGAC11190.11851453312264083No Hit
GCAGGATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTG11140.11798497756802671No Hit
CACAAGTACAGTACATTCAGGAGGCGTTGCTAGGAAACGCTACTGGCTGG11090.11745542201341258No Hit
GGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGG11070.11724359979156693No Hit
GAGGAGGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCC11060.1171376886806441No Hit
CCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACAC11010.11660813312602998No Hit
GGATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGT10940.11586675534957021No Hit
GTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCAC10940.11586675534957021No Hit
GCCGTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCT10800.11438399979665068No Hit
CCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTGGGTGCATTGTGGAT10740.11374853313111372No Hit
GTACAGTACATTCAGGAGGCGTTGCTAGGAAACGCTACTGGCTGGTGAGT10390.11004164424881485No Hit
GCCCTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTC10350.10961799980512354No Hit
CTGCGGGGTTGCTGCCATTCTTAACCCCCCTCCGATCAGAAATGGATTCA10340.10951208869420072No Hit
GGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCG10340.10951208869420072No Hit
AGCGAAAGCAGGGCCATGTGGGTAAGAAGGAGTGGAGATTTATGACAAAA10320.10930026647235508No Hit
TTCTTAACCCCCCTCCGATCAGAAATGGATTCATGCCTGGAACAGCAGAT10320.10930026647235508No Hit
GTGTTACAGTACATTGACCTGAATGACACAAGAGGCAAGCAATGAGGAGG10240.10845297758497248No Hit
GTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTTGTGGAACA10130.10728795536482141No Hit
GATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGA10030.10622884425559316No Hit
GCGGAGGCAGCCCTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACT9920.1050638220354421No Hit
GGCTCAATCTCTGCGGGGTTGCTGCCATTCTTAACCCCCCTCCGATCAGA9910.10495791092451927No Hit
CTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAAT9900.10485199981359644No Hit
GGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCT9890.10474608870267361No Hit
GCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCAC9890.10474608870267361No Hit
GTATACGTACACAAATATCTTTGGAGGCGCAATATAGTTTAAAACAACAC9860.10442835536990515No Hit
GATTATTAACAGCGCCCATGAGACGCACAAGTACAGTACATTCAGGAGGC9820.10400471092621386No Hit
CTCAGGGAGTGTGGTGTGGCATCTGTGTTTGAGAACAATTACAACCCTGT9670.10241604426237147No Hit
CCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCG9640.10209831092960299No Hit
GCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTT9600.1016746664859117No Hit
GGTTGAAGAAGCGTTGCGAAAAACAACGACACACTAAGGCCTACTGTAGC9550.10114511093129758No Hit
GAGAGGAGGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAAC9530.10093328870945192No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCAA100.0070909546144.99895145
GAGCGAA2500.0133.441441
TTAGTAG256.4598134E-6116.0175862
TAGTAGA301.5982094E-596.681313
GCGAAAG25050.092.628812
AGCGAAA26050.088.808761
ACTGGTC3200.088.3587348
CACTGGT3250.086.999377
CGAAAGC27200.085.307053
GTCGCCC950.083.94676145
GAAAGCA29650.078.258074
GACCTAT2650.073.890861
AGGGGTG5750.073.141525
AGGCTCG300.001934545172.510995
GCACTGG4000.072.507156
ATGGGCC1600.067.968254145
GCAGCTC2950.063.8978358
ATCTATA2100.062.162161
CTATACC2150.060.706873
GAATTCG606.792783E-660.435431
Produced by FastQC (version 0.11.5)