FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9350_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9350_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947400
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCG90440.9546126240236437No Hit
GGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGA86150.9093308000844416No Hit
AGCGAAAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTG58530.6177960734642178No Hit
AGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAG50200.5298712265146718No Hit
CCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGT46290.48860037998733374No Hit
ACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCC44040.46485117162761247No Hit
AAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTC43620.4604179860671311No Hit
GCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGT40260.4249525015832805No Hit
GCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGG39580.417774963056787No Hit
AAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGG39130.41302512138484276No Hit
GTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTG38750.4090141439729787No Hit
CTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGA36790.38832594469073256No Hit
AGCGAAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGT34750.36679332911125184No Hit
GTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTG32530.34336077686299343No Hit
GCCGTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGA31630.3338610935191049No Hit
GATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCA31470.3321722609246358No Hit
TTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTG29440.3107451973823095No Hit
GCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAA29350.3097952290479206No Hit
GTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGC29090.3070508760819084No Hit
CTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAG28420.29997888959256913No Hit
CCCCAACCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTGGGTGCATT27990.2954401519949335No Hit
GGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTG27180.2868904369854338No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA26850.28340721975934136No Hit
CCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGC26570.28045176271902045No Hit
GAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCT26200.2765463373443107No Hit
AAGCAGCACTGGTCAGAAATCATTAATAACTGCCCCTCATTTGAAGCACT23020.24298078952923793No Hit
GACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTC22330.23569769896559004No Hit
AAGCAGGGCCATGTGGGTAAGAAGGAGTGGAGATTTATGACAAAAAAGAG21720.2292590246991767No Hit
CCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGA21170.22345366265568925No Hit
GCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTAGTGG20920.22081486172683135No Hit
GCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGTCGGACCT20800.2195482372809795No Hit
TTGTAAAAGTGAGTGTAATTTCATTCAAGTCGCAAACATAAGCACCCTTG20450.2158539159805784No Hit
AAGCAGGGGTGCTTATGTTTGTCGAGGTTTGTAAAAGTGAGTGTAATTTC20250.21374287523749208No Hit
CTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTAGT19920.21025965801139962No Hit
CATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGTGGAGACAG19880.20983744986278235No Hit
GAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCGAGG19610.20698754485961576No Hit
GTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAG19430.20508760819083807No Hit
GTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCAGGCTGTA19050.20107663077897406No Hit
CTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCC18970.2002322144817395No Hit
CACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCT18770.19812117373865318No Hit
ATCTTATAGCGAAGACTACATGGAAATAGCTGTCTTGATTATTAACAGCG18540.19569347688410385No Hit
CCTATAGTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTTGT18530.19558792484694956No Hit
GTCCAATACAAGGCTGACCACTGGGGTAATTACCAAAGCAGCCTCTCTTC18270.1928435718809373No Hit
GCATGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTG18250.19263246780662865No Hit
GCGAAAGCAGGCTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTC18100.1910491872493139No Hit
GGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGC17940.18936035465484485No Hit
GTGCTTATGTTTGTCGAGGTTTGTAAAAGTGAGTGTAATTTCATTCAAGT17830.18819928224614735No Hit
GTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGG17420.18387164872282036No Hit
CCCTTGGTGAGCACCCCCAAGTGTAAGGTTTACTCAAAGAGCCTCTCCTC17370.18334388853704875No Hit
CTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGA16670.17595524593624656No Hit
GTATTAATTAGCATAATTGCAGTGACCGGGCTGCAATTGCTGCGCGGATG16610.17532193371332067No Hit
GGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGC16610.17532193371332067No Hit
GGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACA16280.1718387164872282No Hit
TGACAGTGTCTGCTTGGGCGTCTGCGTGCGCTGCGGAGGCAGCCCTGTCA16280.1718387164872282No Hit
GAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCG16030.16919991555837027No Hit
GGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCCCTGTC15740.16613890648089508No Hit
ATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGTCG15730.16603335444374076No Hit
TCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTT15690.1656111462951235No Hit
CTGCGGAGGCAGCCCTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCA15530.16392231370065444No Hit
GGGTAAGAAGGAGTGGAGATTTATGACAAAAAAGAGATATTACATGCCTC15430.16286679332911125No Hit
CACTAAGGCCTACTGTAGCTAGACGCACTCAGCAGGATGCAGTGTTACAG15320.16170572092041377No Hit
GTGGATATGAGGAGCACAGTGAGGAGCCCATCCAAGGACCACTACAGCCT15040.1587502638800929No Hit
GTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAG14870.15695587924846952No Hit
CCGTAGAGACACTGCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAG14850.15674477517416086No Hit
CCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGATGACG14590.15400042220814864No Hit
GTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGCT14480.15283934979945113No Hit
GATTGAGGCTCAATCTCTGCGGGGTTGCTGCCATTCTTAACCCCCCTCCG14410.15210048553937092No Hit
GAAATGGATTCATGCCTGGAACAGCAGATGAGAAGAAACTCAAGTCGATA14350.15146717331644502No Hit
GGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAA14300.1509394131306734No Hit
TCCCAGCGCCCCAACCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTG14270.15062275701921046No Hit
GAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGC14150.14935613257335867No Hit
TCTTAACCCCCCTCCGATCAGAAATGGATTCATGCCTGGAACAGCAGATG14090.14872282035043277No Hit
CCTCCAAGCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTG14000.1477728520160439No Hit
TCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGA13950.14724509183027232No Hit
ATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAG13900.14671733164450074No Hit
GCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGGCCGAGGTCGAG13850.14618957145872916No Hit
GTGTAAGGTTTACTCAAAGAGCCTCTCCTCTGAAGTGGAGGCCTGCTGTG13820.1458729153472662No Hit
GCGAAAGCAGGGCAGACACGGCCTCCAAGCTCTCCCGCGAGCTGGGTTGC13620.14376187460417986No Hit
GGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCC13510.14260080219548238No Hit
GTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCGTTTCCGT13320.14059531348955034No Hit
GGTCAGAAATCATTAATAACTGCCCCTCATTTGAAGCACTCAGGGAGTGT13300.1403842094152417No Hit
GCCTTGCGTTTCCGTGGAGACAGGGGTTGGGGCGCTGGGATGCGGCAGCC13220.13953979311800715No Hit
GATTAAAGTGGGAGGTGTTGCTTTGTGGAACAGAACACATCTTTTTTTCT13200.13932868904369855No Hit
CATAAGCACCCTTGGTGAGCACCCCCAAGTGTAAGGTTTACTCAAAGAGC13200.13932868904369855No Hit
GACGAGAGGAGGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCC13060.13785096052353812No Hit
CCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCGTG13010.13732320033776652No Hit
ATCTAAGACAGCGGAAGGGCACGGGAGGTTGGCCAAAAGTGTTTTGGTGT13000.1372176483006122No Hit
GCTCTCCCGCGAGCTGGGTTGCGTTCCACCGCCGTCCTCCGTGATGTCGA12860.13573991978045175No Hit
GTAAAAGTGAGTGTAATTTCATTCAAGTCGCAAACATAAGCACCCTTGGT12640.1334177749630568No Hit
GTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCTGCG12630.13331222292590247No Hit
TCTTTATTCAGTGCATTTTTTTATTTCCATTTCTCTCTGCTGCTCTTAGA12450.13141228625712476No Hit
CTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATA12450.13141228625712476No Hit
GCGTTCCACCGCCGTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGC12360.1304623179227359No Hit
GAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGT12340.13025121384842728No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT12320.13004010977411862No Hit
GTGTGAGGGAGAGAGATTGAGTGAGAGAGAGAAAGTTTTGTTCCTCTTAA12220.12898458940257546No Hit
GTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTG12170.1284568292168039No Hit
CCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTTGCG12020.12687354865948913No Hit
AGAGGGAGCGGCGGGACGAGAGGAGGTCTCCACGGTCGCCACGGCTGCCG11900.12560692421363734No Hit
CTCTGATTGGTCGGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAG11800.12455140384209415No Hit
GACCTATAGTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTT11780.12434029976778553No Hit
CTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCCCTGTCTCC11670.12317922735908803No Hit
GGGTTGGGGCGCTGGGATGCGGCAGCCGTGGCGACCGTGGAGACCTCCTC11410.12043487439307578No Hit
GTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATC11350.11980156217014988No Hit
CACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCG11260.11885159383576104No Hit
GATATGAGGAGCACAGTGAGGAGCCCATCCAAGGACCACTACAGCCTGAC11240.1186404897614524No Hit
GCAGGATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTG11160.11779607346421785No Hit
CACAAGTACAGTACATTCAGGAGGCGTTGCTAGGAAACGCTACTGGCTGG11130.11747941735275491No Hit
GAGGAGGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAACCC11090.11705720920413765No Hit
GGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACACTGCAGCTGG11090.11705720920413765No Hit
CCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGAGACAC11030.11642389698121172No Hit
GGATACAGCACTAAGAAGGGGCTCGGGAGCATTTAATGGAGATGTTGGGT10980.11589613679544016No Hit
GTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCAC10980.11589613679544016No Hit
GCCGTCCTCCGTGATGTCGATGACGCATGACAGTGTCTGCTTGGGCGTCT10810.11410175216381675No Hit
CCCCTGTCTCCACGGAAACGCAAGGCAGCGGCGGTGGGTGCATTGTGGAT10780.1137850960523538No Hit
AGCGAAAGCAGGGCCATGTGGGTAAGAAGGAGTGGAGATTTATGACAAAA10480.1106185349377243No Hit
GTACAGTACATTCAGGAGGCGTTGCTAGGAAACGCTACTGGCTGGTGAGT10440.11019632678910703No Hit
GCCCTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTC10380.10956301456618112No Hit
GGCTGTCACCGTGGGAGGATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCG10370.10945746252902681No Hit
CTGCGGGGTTGCTGCCATTCTTAACCCCCCTCCGATCAGAAATGGATTCA10360.1093519104918725No Hit
TTCTTAACCCCCCTCCGATCAGAAATGGATTCATGCCTGGAACAGCAGAT10330.10903525438040955No Hit
GTGTTACAGTACATTGACCTGAATGACACAAGAGGCAAGCAATGAGGAGG10270.10840194215748364No Hit
GTCAGATATCATTCACCAGATTAAAGTGGGAGGTGTTGCTTTGTGGAACA10170.10734642178594046No Hit
GATCTCCGTCTGGAGGAGAAGAGCAGCAAAGCGGCGGCGGTTGCCGTAGA10030.10586869326578002No Hit
GGCTCAATCTCTGCGGGGTTGCTGCCATTCTTAACCCCCCTCCGATCAGA9950.1050242769685455No Hit
GCGGAGGCAGCCCTGTCAGGCTGTAGTGGTCCTTGGATGGGCTCCTCACT9940.10491872493139118No Hit
GTATACGTACACAAATATCTTTGGAGGCGCAATATAGTTTAAAACAACAC9920.10470762085708255No Hit
CTGTAGTGGTCCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAAT9920.10470762085708255No Hit
GCAGCTGGCCGAGGTCGAGAGGGAGCGGCGGGACGAGAGGAGGTCTCCAC9910.10460206881992823No Hit
GGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCT9890.10439096474561958No Hit
GATTATTAACAGCGCCCATGAGACGCACAAGTACAGTACATTCAGGAGGC9860.10407430863415665No Hit
CTCAGGGAGTGTGGTGTGGCATCTGTGTTTGAGAACAATTACAACCCTGT9710.10249102807684189No Hit
CCTTGGATGGGCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCG9640.10175216381676166No Hit
GCTCCTCACTGTGCTCCTCATATCCACAATGCACCCACCGCCGCTGCCTT9630.10164661177960733No Hit
GGTTGAAGAAGCGTTGCGAAAAACAACGACACACTAAGGCCTACTGTAGC9590.10122440363099008No Hit
GAGAGGAGGTCTCCACGGTCGCCACGGCTGCCGCATCCCAGCGCCCCAAC9530.10059109140806417No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAACG100.007091174144.997477
GAGCGAA2750.0105.52511
GGATAGA900.096.7313461
GCGAAAG23250.092.0167852
ATGGGCC950.091.57735145
GTCGCCC406.1178616E-790.62341145
CGAAAGC25150.085.353573
AGCGAAA25400.084.2590941
CACTGGT5400.083.239287
ACTGGTC5400.083.239288
AGTAGAA353.4257988E-582.912581
TAGAGGT1100.079.114584
GAAAGCA27250.078.7758564
GCACTGG6150.074.282686
CTAATAG502.3020493E-672.5217062
ATAGGAG608.014649E-872.52173
GATAGGA608.014649E-872.52172
TAGCTGA502.3034936E-672.5140466
GTTCGTC300.001935845172.49873145
AGGGGTG5450.071.856365
Produced by FastQC (version 0.11.5)