FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9109_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9109_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2113241
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACACATTTGAAAAAAAGACG106030.5017411644010314No Hit
TTCCAACACTGTGTCAAGCTTCCAGGTAGATTGCTTTCTTTGGCATATCC92770.4389939434262349No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT73150.3461507703096807No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA64700.3061647961590751No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG63660.3012434454943852No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT62850.2974104704574632No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC62690.29665333958597245No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA60740.2874258070896788No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG57800.2735135273260362No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC55510.2626770917278247No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG54700.25884411669090274No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC49880.23603554918724368No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT44770.2118546819790076No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG43400.205371748891868No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT38910.18412476381065862No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT38810.18365155701597688No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC38060.18010250605586398No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA34930.165291133382326No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA34280.16221528921689482No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA33830.16008585864082706No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC32840.155401111373478No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT32320.15294043604113303No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA32260.15265651196432398No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT32130.15204134313123774No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG32080.15180473973389688No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG31840.15066904342666074No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA31730.15014851595251086No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT31530.14920210236314743No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA30690.14522716528782093No Hit
TGTTTGAAGAGTCGATAGACACATTTGAAAAAAAGACGATCAAGAATCCA30680.14517984460835276No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGCTTCCAGGTAG30610.14484859985207554No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTTAAC29730.1406843800588764No Hit
GTGTCAAGCTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT29330.1387915528801495No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCATAGGTC29310.13869691152121316No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC29230.13831834608546775No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA28530.1350058985226957No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT28510.13491125716375935No Hit
CTTAAAATGACCATGGTCTCAACACCTGCTTCGAGATACATAACTGACAT28110.13301842998503247No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC27860.13183541299832816No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAATCAGCCACC26900.12729262776938363No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA26440.1251158765138477No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC26230.12412214224501605No Hit
CTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT26220.1240748215655479No Hit
GTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAACTTCTGACCTA26190.12393285952714338No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG26140.1236962561298025No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC26140.1236962561298025No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG25880.12246591846363003No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC25630.12128290147692573No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT25620.12123558079745755No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC25610.12118826011798939No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA25510.12071505332330766No Hit
CCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA25180.11915347090085797No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA24650.11664547488904482No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCGATTTGTACTCCTCTAGTT24310.11503657178712698No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA24200.11451604431297709No Hit
GACATAATGGATTCCAACACTGTGTCAAGCTTCCAGGTAGATTGCTTTCT24170.11437408227457256No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT23720.11224465169850481No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG23690.11210268966010029No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA23010.10888488345626457No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC23010.10888488345626457No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG22870.10822239394371017No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT22730.10755990443115575No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT22540.10666081152126047No Hit
CTTATCTCCTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGT22540.10666081152126047No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA22320.10561975657296069No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA22120.10467334298359723No Hit
GTCGATAGACACATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG21830.10330104327902023No Hit
ATATGGGATCAAGCACTCTTGAACTGAGAAGCGGATACTGGGCCATAAGG21770.10301711920221121No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT21620.10230730901018863No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC21300.1007930472672071No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC100.0070912777145.00095145
TCCGCTC100.0070912777145.00095145
AGCGAAA3800.0101.1406251
GCTTATA301.598131E-596.687881
GCTGACC301.599822E-596.6673145
CGAAAGC4100.093.735643
CTACAAG259.381786E-487.014983
TATATAT353.4360353E-582.867494
AAACTAT2900.080.0061956
AATTCTA2000.076.13455
TTATATT4050.071.617262
TATATTC4200.069.059513
CAGTACG2450.068.060069
ACTATTC3450.067.2452248
CTATTCA3500.066.284589
TAGAAAC15150.063.6578252
GTACTGA808.087227E-963.4424176
AGGGTGT11450.062.6904536
ATATTCA4800.060.4242064
TTCTATT2600.058.5566867
Produced by FastQC (version 0.11.5)