FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9105_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9105_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1643203
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG81630.49677367921066357No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC54350.33075645553227445No Hit
ATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT50490.30726574866282497No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC46450.28267962022951515No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG45650.27781107994569143No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG42960.26144061324133416No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT41230.25091239487756534No Hit
CTCCAATTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT36580.22261400447783994No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG35330.21500691028436533No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG34800.21178150234633214No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA31570.19212477095039382No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA31520.19182048718265485No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA30920.18816908196978707No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC30100.18317882817886774No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC28650.17435459891443722No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT28410.1728940368292901No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC27650.16826892355965756No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC25630.15597585934300265No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT25070.15256788114432607No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA24990.15208102711594368No Hit
CTCATAGACAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC24800.15092474879853554No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA24330.1480644813817891No Hit
GTCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC24010.1461170652682596No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT23650.14392622214053893No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA22830.13893596834961963No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT22200.13510199287610844No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA22050.1341891415728915No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG21990.13382400105160472No Hit
GATAATGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT21760.13242429572000539No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA21370.13005088233164133No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT21190.12895546076778097No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT21120.1285294634929464No Hit
GTTCTTATCTCTTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAA21020.12792089595746842No Hit
CTCATTATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC20980.12767746894327725No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG20800.1265820473794169No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT20800.1265820473794169No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA20680.12585176633684336No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC20560.12512148529426978No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA20060.12207864761687996No Hit
GAATAAGGGATATCCCTAGCAGAATAAGCATCTATTGGACAATAGTAAAA20000.12171350709559317No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG19780.12037465851754164No Hit
ATACATAACTGACATGACTATTGAGGAATTGTCAAGAAACTGGTTCATGC19700.11988780448915928No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA19400.11806210188272537No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC18810.11447155342340538No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG18780.11428898316276198No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC18750.1141064129021186No Hit
CTCTTGTTCCACTTCAAACAGTAGTTGTAATGCTTGCATGAATGTTATTT18390.11191556977439793No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA18120.11027243742860741No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC18060.10990729690732064No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGAAATCTAATGTCGCA17920.10905530235765148No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT17650.10741217001186097No Hit
GTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTT17650.10741217001186097No Hit
AAACAAGGTAGTTTTTTACTCCAATTCTATGCTGACAAAATGACTGTCGT17550.10680360247638301No Hit
ATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTTTTAAC17460.10625589169445285No Hit
TCCCTGTACACATTGTGGTCATAAGTTTCATTTCTTATTGATTCTATGCA17160.10443018908801896No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT17100.10406504856673217No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG17010.10351733778480199No Hit
CTATTAGAAGGGGCCTTATTTTCTCAATTTTCTTCCTTGTTGATTCATTG16750.10193506219255928No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAATTCTATGCTGACAAAATGACT16740.1018742054390115No Hit
GTTCAAGAGTGCTTGATCCCATATTATCCATGTTCTCATTTGAAGCAATT16730.1018133486854637No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC16680.10150906491772471No Hit
ATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGACA16660.10138735141062911No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC16450.1001093595861254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGCC100.0070908996145.00256145
AGCGAAA3400.0102.38591
CGAAAGC4350.081.678053
GAGCGAA1050.075.976841
TATATTC3800.072.51013
TAGCTCC300.001935829472.50128145
AAACTAT2750.071.1895756
TTATATT4100.067.204482
ATATTCA4200.065.604384
CGGATGG451.1939817E-464.44558145
CAGCATG4600.059.890549
ACTATTC3400.057.5727928
TAGAAAC13550.057.25892
AGGGTGT10650.057.189336
AAGGTAC400.006051881754.3743069
GCAGGGT4000.054.3743068
CTATTCA3600.054.3743069
TTCGTAA1101.6771082E-952.728203145
TTCGACC701.6962904E-551.786625145
TAACTAT3950.051.3995635
Produced by FastQC (version 0.11.5)