FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_9060_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9060_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003861
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC102740.512710212933931No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC95780.47797726489012965No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA81730.40786262120975453No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA80900.4037206173482093No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG78630.39239248630518786No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC66640.33255799678720227No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT63620.31748709117049534No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT59950.29917244758992767No Hit
ATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAATT58770.2932838155939958No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT58370.29128766915469684No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA53200.26548747642675813No Hit
CCAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCA52300.26099614693833556No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC52080.25989826639672114No Hit
ACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAA50380.2514146440297007No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC50040.24971791955629655No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC49250.24577553033868116No Hit
GTTTATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGG48070.23988689834274932No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG47450.23679287136183597No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAG46810.23359903705895768No Hit
CAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAA46460.23185240892457112No Hit
TCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACC45930.22920751489250002No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG45900.2290578039095526No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG45550.22731117577516605No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT44190.22052427788154966No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT43560.21738034723965383No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA43130.2152344898174075No Hit
CCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG42990.21453583856365288No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT42020.20969518344835297No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT41420.20670096378940456No Hit
CTTCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACA38160.19043237030911825No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA38000.18963391173339866No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA36910.1841944126863091No Hit
CCCTTATTCCCATGGAACGGGAACAGGCTACACAATAGACACCGTGATCA35120.17526165737044636No Hit
CACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCTTTGACATTCTTCCT34780.17356493289704228No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT33980.1695726400184444No Hit
GCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATC33240.16587976910574137No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC32140.1603903663976693No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA31120.155300192977457No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT30740.15340385386012304No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA30550.15245568430145603No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT30550.15245568430145603No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC29400.1467167632884716No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG28950.1444710985442603No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG28290.14117745691941708No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT28140.14042890200467997No Hit
GAACACATGAGTACTCGAACAAAGGAAAACAGTATGTTTCTGACATCACA27400.13673603109197693No Hit
TTCCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTA27380.136636223770012No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA27380.136636223770012No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT27080.1351391139405378No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT26020.1298493258763956No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG25810.12880134899576368No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA25690.12820250506397401No Hit
GATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGT25590.12770346845414926No Hit
GTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCA24970.12460944147323592No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT24740.12346165727063904No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG24620.12286281333884934No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA24540.12246358405098956No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA24380.12166512547526999No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA24290.12121599252642773No Hit
TATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATCATC24140.12046743761169063No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC24060.12006820832383083No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT23900.11926974974811126No Hit
GTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT23140.1154770715134433No Hit
TGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCT22620.11288208114235468No Hit
TATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGC22510.11233314087154748No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA22170.11063641639814338No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA21960.10958843951751145No Hit
GAATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAA21740.10849055897589703No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC21680.10819113701000219No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG21540.10749248575624756No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT21530.10744258209526508No Hit
ATTGTACACCCTGTGATGTCAGAAACATACTGTTTTCCTTTGTTCGAGTA21490.1072429674513352No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT21210.10584566494382594No Hit
GGCTACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGG21170.10564605029989606No Hit
CTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTT20900.10429865145336926No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT20830.10394932582649195No Hit
ATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAGTA20680.10320077091175485No Hit
GAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAA20670.10315086725077238No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT20640.10300115626782497No Hit
TTCTTAATCTATGGGCCATAGTGCCGTTGCAGCAGGCTGGTTTCTGCATA20580.10270173430193012No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACAAAAAATCTCAATT20430.10195317938719302No Hit
GTCTTCAGGTAATGGCCCATTTGTTGGATCTATCATTGTACACCCTGTGA20280.10120462447245591No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT20230.10095510616754357No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG20110.10035626223575388No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCAT100.007090605145.00537145
GAGCAGA2300.0129.282621
AGCACGC6150.0119.105121
GCACGCA6300.0116.246062
CACGCAC6350.0115.3307343
GAAACAC10750.0113.989494
GGCACCC203.8660603E-4108.75402145
ATAAGCC203.8660603E-4108.75402145
AAACACG11350.0107.324785
AACACGT4150.0104.825776
ACACGTG4550.095.6031657
TAGAAAC13800.093.527292
GCACAGC406.114751E-790.63742
AACACGA8850.090.118586
ACACGAG9200.086.683667
ACAACGC3800.080.130548
CGGTGCA651.5643309E-978.087612
CAACGCA4000.076.124029
ACGTGCC5200.075.287499
CACGAGC10650.074.881668
Produced by FastQC (version 0.11.5)