FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_9060_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_9060_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2011319
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC103170.5129469765860114No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC96120.4778953512595466No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA82020.40779210060661686No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA81160.40351629950296297No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG79000.392777078126344No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC66840.3323192392653776No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT63810.31725449816762036No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT60190.2992563586382866No Hit
ATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAATT58980.2932404059226806No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT58620.2914505356932441No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA53450.26574601045383656No Hit
CCAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCA52590.26147020935018267No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC52240.2597300577382305No Hit
ACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAA50600.25157620447079754No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC50230.24973661562387667No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC49450.24585856346009757No Hit
GTTTATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGG48220.23974317350952284No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG47710.23720752401782114No Hit
CAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAG46940.23337919047152642No Hit
CAATAACCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAA46640.23188763194699596No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG46120.2293022638378099No Hit
TCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACC46060.22900395213290384No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG45610.22676661434610823No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT44320.22035291269062737No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT43720.21736979564156655No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA43290.21523189508973964No Hit
CCCCATGGACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG43110.21433695997502134No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT42150.209563972696524No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT41560.20663057426494755No Hit
CTTCATAGATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACA38260.1902234304951129No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA38140.18962680708530075No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA37050.18420747777950688No Hit
CCCTTATTCCCATGGAACGGGAACAGGCTACACAATAGACACCGTGATCA35250.17525812663232437No Hit
CACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCTTTGACATTCTTCCT34890.17346825640288785No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT34130.16968964147407745No Hit
GCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATC33440.1662590568676575No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC32240.16029282276953583No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA31250.15537067963858545No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT30880.15353109079166458No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA30690.15258643705946198No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT30670.15248699982449326No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC29450.1464213284914029No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG29110.1447308954969351No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG28380.14110143642057774No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT28280.14060425024573425No Hit
GAACACATGAGTACTCGAACAAAGGAAAACAGTATGTTTCTGACATCACA27540.13692507255189257No Hit
TTCCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTA27470.13657704222950212No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA27460.13652732361201778No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT27170.13508548370497173No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT26170.13011362195653697No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG25930.12892037513691265No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA25770.1281248772571631No Hit
GATGTACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGT25690.1277271283172883No Hit
GTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGCCA25050.12454513679829007No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT24840.12350104583111877No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG24740.1230038596562753No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA24610.12235751762897878No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA24460.12161173836671359No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA24420.1214128638967762No Hit
TATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATCATC24240.12051792878205794No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC24180.12021961707715187No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT23990.11927496334494926No Hit
GTAGAAACACGTGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT23230.11549634841613887No Hit
TGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAATCATCCT22700.11286126168946846No Hit
TATCACATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGC22580.11226463827965628No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA22200.1103753308152511No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA22060.10967927017047022No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC21830.10853574196833024No Hit
GAATATAAATCCGTATTTTCTCTTCATAGATGTACCTATACAGGCAGCAA21780.1082871488809085No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG21640.10759108823612763No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT21610.1074419323836746No Hit
ATTGTACACCCTGTGATGTCAGAAACATACTGTTTTCCTTTGTTCGAGTA21530.10704418344379982No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT21260.10570178077172245No Hit
GGCTACACAATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGG21230.10555262491926941No Hit
CTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTT20990.10435937809964507No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT20880.10381247330731723No Hit
GAGCTTATGTGTACCCAATCTCACCAAGGTGAGCCATTGCTTTTTCAAAA20770.10326556851498943No Hit
ATAGACACCGTGATCAGAACACATGAGTACTCGAACAAAGGAAAACAGTA20740.10311641266253638No Hit
TTCTTAATCTATGGGCCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT20700.10291753819259898No Hit
TTCTTAATCTATGGGCCATAGTGCCGTTGCAGCAGGCTGGTTTCTGCATA20670.10276838234014593No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACAAAAAATCTCAATT20460.10172429137297465No Hit
GTCTTCAGGTAATGGCCCATTTGTTGGATCTATCATTGTACACCCTGTGA20340.10112766796316248No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT20290.10087907487574074No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG20240.10063048178831902No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGC100.00708916145.015266
TATTATA100.0070902137145.00806145
GCAGCCC100.0070902137145.00806145
GAGCGCG100.007091795144.997247
GAGCAGA1900.0133.59991
AGCACGC6150.0110.8522341
CACGCAC6300.0108.194063
GCACGCA6300.0108.194062
GAAACAC10100.0106.254094
AAACACG10300.0104.190915
GCACAGC352.759807E-7103.5900652
CGGTGCA352.759807E-7103.5900652
ATAAGCC301.5994134E-596.672035145
AACACGT4250.095.5394746
ACGGACC406.117716E-790.630035145
AACACGA7450.090.512886
ACACGTG4550.089.229087
GAGCCCC259.386067E-487.00483145
TAGAAAC13400.086.582742
ACAACGC3550.083.7308048
Produced by FastQC (version 0.11.5)