FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_895_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_895_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938888
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT166200.8571923700595393No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA89230.4602122453695108No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA72760.37526664768671525No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT61310.3162121793522885No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT61250.31590272362302513No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT59470.30672220365487846No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC53280.274796687585874No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT51710.26669926267014904No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG50760.26179954695681235No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT50430.2600975404458638No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT46260.2385903672620595No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC44430.22915196751952666No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG43190.22275654911475032No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA42650.21997144755137996No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT42450.21893992845383542No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT41700.21507173183804326No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC41560.21434966846976206No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT38800.20011470492364694No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT38330.1976906350444172No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT37330.19253303955669437No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT36900.19031527349697352No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA35880.1850545260994962No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA34330.17706025309352577No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT33320.1718510816509257No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC33150.1709742904180128No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA32690.16860179649366028No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC31610.1630315933669196No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT30300.15627514327800265No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC29810.15374792148901845No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA29700.15318058598536893No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG28940.14926081341469957No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG27260.14059605299532515No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC26450.13641840065026964No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA25560.1318281406661963No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG25020.12904303910282594No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT24610.12692842495285958No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC24440.12605163371994668No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT24070.12414332338948923No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT24040.12398859552485754No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA23780.12264762069804962No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT23750.12249289283341792No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA23080.1190373038566436No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG22790.11754160116520397No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG22650.11681953779692276No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA22600.11656165802253662No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT22290.11496280342134255No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA22160.11429231600793856No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA21710.11197139803846329No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT21560.11119775871530485No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC21540.1110946068055504No Hit
AAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACT21350.11011466366288306No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA21240.10954732815923354No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG21000.10830950524218005No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA20950.1080516254677939No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT20640.10645277086659982No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT20400.10521494794954633No Hit
AGCACGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGTA20300.10469918840077404No Hit
GCTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA19960.10294560593494828No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAGTTACAAAAAACC19750.10186251088252647No Hit
GTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTC19730.10175935897277202No Hit
CAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATAAGA19410.10010892841670072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGG100.0070829587145.057591
TAATACG100.0070873303145.027654
GTAGAGC100.0070873303145.027653
AGCACGC21000.0129.51571
CACGCAC21150.0128.570623
GCACGCA21150.0128.570622
GAAACAC5450.0123.739184
AAACACG5850.0115.278395
GAGCAGA1150.0113.523321
CGGTGCA458.367351E-11112.799282
AACACGT1250.0110.2124946
ACGTGCA2050.099.0432742
CCTGTCC451.0923031E-896.66515145
ACGCACT28800.095.677964
CGCACTT29000.095.2681665
ACAACGC7400.087.194578
CCCCGTC259.3809795E-487.0165942
AACACGA6200.086.542066
ACACGTG1600.086.09247
CAACGCA7600.084.899999
Produced by FastQC (version 0.11.5)