FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_874_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_874_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286423
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC9430.3292333367082951No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT8300.2897811977390084No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT7500.26185047988464616No Hit
CTTATGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAG7180.25067819274290126No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA6950.2426481113597721No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT6580.22973015435212957No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA5660.19760982881961298No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA5420.18923061346330428No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC5210.1818988000265342No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC5170.18050226413381606No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG5150.17980399618745702No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT5000.1745669865897641No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT4970.17351958467022552No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC4930.1721230487775074No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT4490.15676115395760815No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT4280.14942934052083806No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT4280.14942934052083806No Hit
GAATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTTATG4200.14663626873540184No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG4200.14663626873540184No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT4180.1459380007890428No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC4120.1438431969499656No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC4050.14139925913770893No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA4030.14070099119134985No Hit
ACTAAGGGCTTTCACTGAGGAGGGGGCAATAGTTGGAGAAATTTCACCAT3960.13825705337909316No Hit
GGTCTATTCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT3920.13686051748637504No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC3920.13686051748637504No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG3850.13441657967411835No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG3820.13336917775457977No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGC3710.12952870404960495No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA3690.1288304361032459No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT3670.12813216815688683No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT3640.12708476623734824No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT3600.12568823034463014No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC3600.12568823034463014No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA3570.12464082842509157No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG3520.12289515855919392No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT3420.11940381882739864No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC3340.1166107470419624No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG3300.1152142111492443No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG3270.1141668092297057No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC3260.1138176752565262No Hit
CTACTAAGGGCTTTCACTGAGGAGGGGGCAATAGTTGGAGAAATTTCACC3250.11346854128334664No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC3240.11311940731016713No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC3220.11242113936380807No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT3200.11172287141744902No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGGGCAATAGTTGGAGAA3180.11102460347108996No Hit
GTTTAATTTTGCTTCCTCTGAGTATTTTGGGTAGTCATAAGTCCCATTTT3120.10892979963201278No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG3080.10753326373929468No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC3070.10718412976611516No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA3060.10683499579293562No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTGGAA3060.10683499579293562No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG3060.10683499579293562No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA3050.1064858618197561No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC3040.10613672784657657No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT3030.10578759387339703No Hit
CTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC3030.10578759387339703No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC3030.10578759387339703No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT3020.10543845990021751No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC3020.10543845990021751No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA2990.10439105798067892No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGAACCCC2980.10404192400749941No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT2950.10299452208796082No Hit
GTTTTTTAACTGGTTTCTTACTTTTTCATACAAGTTCTTCACATTTGAAT2950.10299452208796082No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG2930.10229625414160176No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT2930.10229625414160176No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC2890.10089971824888365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACT458.367351E-11112.7766954
AGTAGAA850.0110.997551
CGAAAGC750.0106.350873
GCGAAAG750.0106.350872
CCTTATA501.9463187E-10101.516752
ACTCAGC301.5892829E-596.76711
CAGGTAC259.3737146E-487.014363
GTAGAAA2100.082.943221
CTCAGCA353.4302735E-582.870812
CAGGGTG554.3835826E-879.090149
ATATAGT809.822543E-1172.5753251
AGGTACT300.001934401572.49934
GCACCGT406.6553956E-572.49936
GCAGGGT608.011739E-872.49938
ACCGTCT406.6553956E-572.49938
CACCGTC406.6553956E-572.49937
TCCTAAT350.003549272462.2074171
ATATTCA350.003564042762.1422584
ATAGTGG954.5292836E-1061.0627023
CAAGGTA1154.0017767E-1156.7385838
Produced by FastQC (version 0.11.5)