FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_822_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_822_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194854
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC88490.40317032476875453No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT88450.40298808030055755No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG81440.371049737249038No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT70160.31965679721749146No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA61220.2789251585754679No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC56110.2556434277633045No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT55600.2533198107937931No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG52280.2381935199334443No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT51550.23486755838884954No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC47190.21500291135537944No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA43550.19841866474945485No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT41330.18830409676452284No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC40320.18370242394254924No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG38860.17705050085335972No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC38450.17518249505434075No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT38210.1740890282451589No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC37300.16994296659367777No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC37170.1693506720720376No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA36650.16698149398547696No Hit
GTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC36500.16629807722973827No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA36350.16561466047399964No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT35030.15960059302349952No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT34900.15900829850185935No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTATGTCCCCCTGC34740.15827932062907146No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT34320.15636575371300324No Hit
GAATAAAAGAGCTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA34100.15536340913791988No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT33700.15354096445595014No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT32650.1487570471657796No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG32550.14830143599528714No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG32510.1481191915270902No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT32070.14611450237692347No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA31330.14274297971527947No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA31160.14196844072544235No Hit
AATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCGCA31100.14169507402314688No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA30350.1382779902444536No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC29760.13558988433854827No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA29530.13454197864641568No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA29480.13431417306116944No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC28710.1308059670483777No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT28200.1284823500788663No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT27810.1267054665139458No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA27810.1267054665139458No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGATCCAGC27440.1250197051831238No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA27270.12424516619328667No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT27030.12315169938410483No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT26990.12296945491590784No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG26890.12251384374541541No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC26770.1219671103408245No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA26600.12119257135098735No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT26250.11959793225426385No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT26180.11927900443491914No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG26120.11900563773262368No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG26080.1188233932644267No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG25980.11836778209393427No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC25930.11813997650868804No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG25880.11791217092344183No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG25790.11750212086999864No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGAT25780.1174565597529494No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG25760.11736543751885091No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT25720.11718319305065393No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA25380.11563411507097966No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC25260.11508738166638875No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC25180.1147228927299948No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG24590.11203478682408943No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT24530.11176142012179398No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT24350.1109413200149076No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC24320.11080463666375986No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC23980.1092555586840856No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA23950.10911887533293786No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAAATTGCAACACTTCT23780.10834433634310071No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT23720.10807096964080526No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC23510.10711418618277115No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG23500.10706862506572191No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA23470.10693194171457418No Hit
GTCCTACATTGTGGAAACATCTAATTCAAACAATGGAACGTGTTACCCAG23290.1061118416076878No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGACTCCAGC23270.10602071937358931No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC23250.10592959713949084No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC23250.10592959713949084No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA23160.10551954708604763No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG23130.1053828637348999No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG23080.1051550581496537No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACC22400.10205690219030515No Hit
TTCTTGATCCGTCCAGACTCAAAGTCGACTCTGGCATCAATCCGGGCCCT22400.10205690219030515No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT22360.10187465772210817No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT22150.10091787426407405No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG22110.10073562979587708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGGG151.2301642E-4145.00117145
GGTCGTC100.007091745144.997887
GAGCGAA700.0124.334881
AGTAGAA9500.0112.992041
CCGCACC352.7627175E-7103.57227145
CAGGTAC1100.098.877953
GTACTGA1200.090.6346
GCGAAAG5900.087.258412
CGAAAGC6250.082.371933
ATTGGGG702.8139766E-972.500595145
TATGCCC300.00193598972.50059145
GTAGAAA18650.069.223081
ATATTCA8250.066.7975544
AGGTACT1700.063.979854
GGTCAAA5850.060.7505231
GTGTTTT18800.060.1586959
TAGATAT4100.053.0564653
CATACTC7800.052.9884453
TTCTACT1650.052.7349134
GGACAAT2650.051.9862946
Produced by FastQC (version 0.11.5)