FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_822_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_822_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2202164
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT88770.403103492746226No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC88740.4029672631102861No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG81690.3709532986644047No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT70390.3196401357937011No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA61330.278498785739845No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC56300.2556576167805849No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT55800.2533871228482529No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG52480.23831104313756835No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT51690.23472366272448372No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC47390.2151974149064284No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA43780.19880444871499126No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT41430.18813312723303077No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC40470.1837737788829533No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG39080.17746180575107032No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC38590.17523672169738494No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT38360.1741922944885122No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC37360.16965130662384817No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC37320.16946966710926162No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA36790.1670629435409897No Hit
GTCCATGCACGTGTTATCGCATTTGTGGTAAAATTCAAAGCAGCCGTTTC36580.16610933608941023No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA36460.16556441754565057No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT35150.15961572344294067No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT35010.15897998514188771No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTATGTCCCCCTGC34790.15798096781166163No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT34450.15643703193767586No Hit
GAATAAAAGAGCTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA34190.15525637509286322No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT33790.1534399799469976No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT32730.1486265328104537No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG32650.1482632537812806No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG32600.1480362043880474No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT32170.14608357960624185No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA31400.14258701895045056No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA31260.1419512806493976No Hit
AATATATTCAATATGGAGAGAATAAAAGAGCTGAGAGATCTAATGTCGCA31180.14158800162022447No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA30470.138363900236313No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC29870.13563930751751457No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA29610.13445865067270193No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA29580.13432242103676204No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC28840.13096209001691064No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT28280.1284191368126988No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA27910.1267389713027731No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT27900.12669356142412644No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGATCCAGC27480.12478634652096755No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA27380.12433224773450115No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT27100.12306077113239523No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT27100.12306077113239523No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG27000.12260667234592881No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC26860.12197093404487586No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA26740.12142601550111616No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT26410.11992748950577704No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT26260.11924634132607745No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG26220.11906470181149088No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG26160.11879224253961104No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG26050.11829273387449799No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC26000.1180656844812648No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG25970.11792945484532487No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTTGAT25850.11738453630156517No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG25840.11733912642291854No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG25840.11733912642291854No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT25810.11720289678697864No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA25480.11570437079163949No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC25320.11497781273329326No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC25310.11493240285464662No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG24650.11193535086396836No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT24580.11161748171344187No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC24420.11089092365509563No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT24400.11080010389780234No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC24100.10943780753840313No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA24040.1091653482665233No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAAATTGCAACACTTCT23900.10852960996547033No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT23770.10793928154306401No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC23580.10707649384877785No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG23560.10698567409148456No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA23530.10684944445554465No Hit
GTCCTACATTGTGGAAACATCTAATTCAAACAATGGAACGTGTTACCCAG23370.1061228863971984No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGACTCCAGC23350.10603206663990512No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC23310.10585042712531856No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC23290.10575960736802527No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA23210.10539632833885214No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG23190.10530550858155886No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG23120.1049876394310324No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACC22480.10208140719764741No Hit
TTCTTGATCCGTCCAGACTCAAAGTCGACTCTGGCATCAATCCGGGCCCT22470.10203599731900077No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT22420.10180894792576757No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT22220.10090075035283476No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG22220.10090075035283476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCAGA100.0070892065145.015262
CCTCGCC253.713103E-8144.9988145
GAGCGAA1050.0138.141141
CAGGTAC1300.0105.972693
AGTAGAA10150.099.318721
CCGCACC651.6370905E-1189.23003145
GTACTGA1550.088.87836
GCGAAAG6650.088.317562
CGAAAGC6700.087.658483
GTAGAAA20800.072.872771
TTCTACT1700.072.507634
ATTGGGG702.8157956E-972.499405145
CAGGTCA2050.063.6580129
AGGTACT2400.063.4441764
TTTCTAC2000.061.6314853
GCAGGTA3100.058.4738922
TTAACAG4850.055.30882145
AGATATT4350.055.005794
TAGATAT4300.053.9591643
TAGAAAC29100.052.0759242
Produced by FastQC (version 0.11.5)