FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_709_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_709_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567159
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA45940.29314192114520604No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA38020.24260461127428679No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT37890.24177508472337522No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT34340.21912262891002127No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA34000.21695309793071413No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT31460.20074542532059605No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC29390.1875368102406967No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC28110.17936916420095217No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC27590.17605105799730594No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG26890.17158437656932066No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT26650.17005294293686857No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG26400.16845769956973097No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT26210.16724531461070638No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC26040.1661605491210528No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA25440.16233196503992256No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA25220.16092815087684148No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA25120.16029005352998643No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA24820.1583757614894213No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT24260.15480241634703307No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA24070.15359003138800847No Hit
GTTAGTAGCAGATGGTGGGCCCAACATTTACAACTTGAGAAACTTACATA23810.15193097828618538No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG23750.15154811987807237No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC23620.1507185933271608No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC23510.15001668624562026No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC23460.14969763757219273No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC23440.14957001810282172No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA23360.1490595402253377No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT22070.14082808445090766No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG21270.13572330567606733No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT20570.13125662424808204No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG20390.13010804902374296No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC19700.12570517733044317No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT19530.1246204118407896No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA19520.1245566021061041No Hit
TTCTTAATCTATGGGTCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT19400.12379088528987806No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC19380.12366326582050705No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT19320.12328040741239402No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG19290.1230889782083375No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA19020.1213661153718289No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC18900.12060039855560285No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG18480.11792038969881168No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA18290.1167080047397871No Hit
TTATAAAGGAGCTATGGGAAAACACTAAAGGAAGAGATGGTCTGTTAGTA18230.11632514633167405No Hit
CATCAAAAGAGCATTGTCATTAAACACAATGACAAAAGATGCTGAAAGGG18130.11568704898481902No Hit
TATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGT18070.11530419057670599No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC18010.11492133216859297No Hit
ACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCACCCTCAAAATCCC17710.11300704012802784No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC17580.11217751357711629No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT16910.10790226135318753No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC16860.10758321267976001No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA16830.1073917834757035No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA16700.10656225692479195No Hit
ATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACA16690.10649844719010644No Hit
GGTTATATCTGCTTCTTTGATGCCTTCAATAGACAAATGTCCTACAAAGG16610.10598796931262239No Hit
GTTCTACAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT16520.10541368170045284No Hit
GTTCCAGACACTGCATCGTAATCCATTTTCCTCACAGGACCATGTGCTGG16050.10241462417023416No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC16040.10235081443554865No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA15940.10171271708869362No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG15890.10139366841526609No Hit
GTTCATAGCTGATTATAGGTACACATACAAATGCCACAGAGGAGATTCCA15890.10139366841526609No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGGATCTTTTTGGCTATGACT15840.10107461974183858No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT15690.10011747372155602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATAC100.007091495144.998287
ATAAGCC100.007091495144.99828145
CAACGCA1850.0101.8906869
AGCGTCG451.0921212E-896.665538
AACAACG2050.091.950137
AAACACG8400.090.635495
TAGAAAC9650.087.160352
GCACTTA259.3834323E-487.010086
ACTTCAG502.269735E-886.998978
AACACGA6500.075.8549356
ACGCACT5800.070.00814
CGCACTT5800.070.00815
GAAACAC11400.067.420084
ACGTGCC1100.065.908319
GAGCGTC702.3355824E-762.142127
ACAACGC3100.060.805738
TAGTAAC10600.057.459482
ATCCGTA3750.056.0659987
AATCCGT3900.055.775696
CACTTCT400.006038833454.4038621
Produced by FastQC (version 0.11.5)