FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_709_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_709_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574441
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA46150.2931199073194867No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA38160.242371737016503No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT38000.24135550331832062No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT34420.21861727432148934No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA34120.21671183613739733No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT31600.2007061553910245No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC29540.18762214652692605No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC28270.17955579154760323No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC27730.17612600281623764No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG27050.17180700959896242No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT26770.17002860062714323No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG26600.16894885232282442No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT26280.1669163849264596No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC26190.166344753471232No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA25550.1622798186785024No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA25400.16132709958645639No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA25240.160310865888274No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA25000.1587865153410004No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT24320.15446752212372516No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA24190.15364183224395198No Hit
GTTAGTAGCAGATGGTGGGCCCAACATTTACAACTTGAGAAACTTACATA23880.15167287945372357No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG23850.15148233563531438No Hit
GTGTACAATGATAGATCCAACAAATGGGCCATTACCTGAAGACAATGAGC23710.15059313114940476No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC23640.15014852890644997No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC23550.14957689745122238No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC23500.14925932442054038No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA23470.14906878060213116No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT22140.14062133798598994No Hit
CATGTATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATG21340.13554016949507794No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT20680.13134820549007553No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG20510.13026845718575672No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC19780.12563189093779953No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT19620.12461565723961711No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGCTACGCTA19610.12455214263348072No Hit
TTCTTAATCTATGGGTCATAGCCTCAAGCATGCTATGTTGCCCTACTGGT19490.12378996735984392No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC19450.1235359089352983No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGGCAACATAGCATGCTT19370.1230277920862071No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG19360.1229642774800707No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA19060.1210588392959787No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC18980.12055072244688748No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG18610.1182006820198407No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA18390.1168033606848399No Hit
TTATAAAGGAGCTATGGGAAAACACTAAAGGAAGAGATGGTCTGTTAGTA18340.1164857876541579No Hit
TATTTCACCTCCGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGT18160.11534252474370268No Hit
CATCAAAAGAGCATTGTCATTAAACACAATGACAAAAGATGCTGAAAGGG18160.11534252474370268No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC18080.11483440789461148No Hit
ACCTAATGGACCCTGAATACAAAGGGCGGTTACTTCACCCTCAAAATCCC17760.11280194049824667No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC17640.11203976522460987No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT17070.10841943267483507No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA16950.10765725740119828No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC16930.10753022818892548No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA16750.10638696527847026No Hit
ATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACA16740.10632345067233386No Hit
GGTTATATCTGCTTCTTTGATGCCTTCAATAGACAAATGTCCTACAAAGG16730.10625993606619746No Hit
GTTCTACAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT16590.10537073158028785No Hit
GTTCCAGACACTGCATCGTAATCCATTTTCCTCACAGGACCATGTGCTGG16120.10238554509187706No Hit
GTATGCAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC16100.10225851587960426No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA16050.10194094284892226No Hit
GTTCATAGCTGATTATAGGTACACATACAAATGCCACAGAGGAGATTCCA15950.10130579678755826No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG15940.10124228218142185No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGGATCTTTTTGGCTATGACT15870.10079767993846706No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT15750.10003550466483024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAAC10450.094.368012
AAACACG9850.094.224145
AACACGA6900.091.420756
ACGAGCC2650.087.546219
CGAGCGA259.3822455E-487.012863
ACTTCAG300.001936033672.499218
CGATGTT406.664737E-572.499217
AACAACG2000.072.49927
CAACGCA2000.072.49929
GAAACAC13200.070.3111954
ACGTGCC1350.069.814059
CGCACTT6500.069.161875
ACGCACT6550.068.633934
AAGCGGA1150.063.0528032
TAGTAAC12050.062.5818632
ATATTAG2050.060.1289335
CACGAGC10650.059.22478
GAAGCAC1350.059.0809334
TATATTA2100.058.697294
CTCAGAC902.048182E-856.3972243
Produced by FastQC (version 0.11.5)