FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_703_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_703_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013464
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAG81320.8023965330786293No Hit
GTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTG80330.7926280558559553No Hit
CTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACA58220.5744653978829045No Hit
GTAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCC51830.5114143176274638No Hit
GGTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTT39310.387877615781123No Hit
AAACTATAATGCGCAAATTCTACTCTGCTTGTAATACACACTGTATGCTA39270.3874829298327321No Hit
GATATAGACAGAGCAGAGAGAGGCGCCAAGCAATGGAATGAAGAAGGGAG38900.3838320848101166No Hit
ATTCTACTCTGCTTGTAATACACACTGTATGCTAGCCTATTTACCCGTAA35330.3486063639162319No Hit
ATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAGTA34890.34426481848393237No Hit
ATATAAACTAATGCCGTCATTCACATAGTTTTCTGCTTCATTGCGCTGCT28630.2824964675607619No Hit
AATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGT27070.26710371557351814No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC24520.2419424863636005No Hit
GCTTGTAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAA21870.21579454228270564No Hit
CTCTTATTCAGTCTCTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT20760.20484200721485912No Hit
CTATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAATGACG19840.19576423040186924No Hit
TCCTTAAGGAGAACATTAACCGATCAGGGGTAGAAGCCACCTCTTGCCTT19250.18994261266310397No Hit
ATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAATGACGGC17930.1769179763662054No Hit
GAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAGTAGGTTTACAGT17710.17474720365005567No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT17620.1738591602661762No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT17060.16833355698870409No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16680.16458404047899086No Hit
GCTTACAGCTGCACATTCCCACCAAACTATAATGCGCAAATTCTACTCTG16310.16093319545637536No Hit
GTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACATA16200.1598478090983005No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT15490.15284213351436263No Hit
CCATTAAAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG15330.15126338972079917No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA14770.14573778644332702No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG14600.14406037116266585No Hit
GTACTGGAAGGGACCTGCAAGCTGTAGAGAAAACACAAGGAAGGGTAAGA14430.14238295588200467No Hit
GGCTAATAGATTTCTTAAAGGATGTGATGGAATCAATGAACAAAGAGGAA14380.1418895984465161No Hit
TTGTAGAACCGCTTAGTAGGTTTACAGTACACTATTATAAGGATAAGCAG13960.137745395988412No Hit
TATTAGCACTCATATGGCATATGGACATGTTGCATACATGATTCCTTGAA13890.13705469557872801No Hit
TTATAATAGTGTACTGTAAACCTACTAAGCGGTTCTACAACCAATACTTT13610.13429189393999194No Hit
AAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTT13510.1333051790690148No Hit
ATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACATAGT13210.1303450344560833No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA13120.12945699107220385No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC12970.1279769187657381No Hit
CCTTATGACTCACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA12580.12412873076892716No Hit
CGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACC12440.12274732994955913No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTCTGT12430.12264865846246142No Hit
CCATAGAAGTTTTTAGATCGAATGACCTAACAGCTAACGAGTCAGGAAGG12300.12136592913019112No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC12100.11939249938823678No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAAATTGCAACACTTCT12100.11939249938823678No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT12050.11889914195274819No Hit
ACTATAATGCGCAAATTCTACTCTGCTTGTAATACACACTGTATGCTAGC12030.11870179897855276No Hit
CATTAAAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA11870.11712305518498932No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC11620.11465626800754639No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCCATTAAAAGAGGCCTTATTTTC11500.1134722101623738No Hit
GTATTGGTTGTAGAACCGCTTAGTAGGTTTACAGTACACTATTATAAGGA11220.11070940852363775No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAAACGAGAAGATCATTT11130.1098213651397583No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG11030.10883465026878113No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG10950.1080452783719994No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT10770.1062691916042405No Hit
AAATAAGGTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTA10640.1049864622719702No Hit
CCCATAATGTTCTCAAATAAAATGGCAAGACTAGGAAAAGGGTACATGTT10600.10459177632357933No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT10530.10390107591389533No Hit
CTTCAGGAATGTGAAGATTCCGTATGTTGTATAAGTTTGGTCCTCCATCT10420.10281568955582043No Hit
GTACACTATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAA10390.1025196750945273No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT10370.10232233212033184No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC10260.10123694576225697No Hit
CTCATATGGCATATGGACATGTTGCATACATGATTCCTTGAAACATAAAA10180.10044757386547525No Hit
TAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCG10180.10044757386547525No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA100.007089167145.011691
ATAGCCG100.007089167145.011693
TAGCCGT100.007089167145.011694
GTGCGGC100.0070912587144.997399
GCTCGAC203.8663496E-4108.748047
AGGCTCG259.383777E-487.007025
CGAAAGC5350.082.670223
GATATAG7850.076.662231
ATATAGA7950.075.697932
TATAGAC8000.075.224823
ATAGACA8100.074.296124
ACAAGGC809.822543E-1172.4986958
GTAGAAA5650.069.297621
ACAGAGC8800.068.379458
GATCTAT554.0597006E-665.9144061
GGCGAAA451.1933898E-464.449641
GTCCTAG702.3332723E-762.147871
AAGGTCC350.003566771762.141749
CTGAGGG502.009456E-458.004684
CTAGACG753.75916E-758.0046774
Produced by FastQC (version 0.11.5)