FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_703_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_703_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016675
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAG81560.8022229325989132No Hit
GTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTG80580.7925836673469889No Hit
CTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACA58370.5741264415865444No Hit
GTAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCC51950.5109794182014902No Hit
AAACTATAATGCGCAAATTCTACTCTGCTTGTAATACACACTGTATGCTA39420.38773452676617404No Hit
GGTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTT39380.3873410873681363No Hit
GATATAGACAGAGCAGAGAGAGGCGCCAAGCAATGGAATGAAGAAGGGAG39000.383603413086778No Hit
ATTCTACTCTGCTTGTAATACACACTGTATGCTAGCCTATTTACCCGTAA35400.3481938672633831No Hit
ATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAGTA34960.34386603388496817No Hit
ATATAAACTAATGCCGTCATTCACATAGTTTTCTGCTTCATTGCGCTGCT28710.2823911279415742No Hit
AATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGT27150.2670469914181031No Hit
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC24600.24196522979319843No Hit
GCTTGTAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAA21950.21589986967319938No Hit
CTCTTATTCAGTCTCTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT20790.2044901271301055No Hit
CTATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAATGACG19920.19593282022278505No Hit
TCCTTAAGGAGAACATTAACCGATCAGGGGTAGAAGCCACCTCTTGCCTT19330.19012958910172867No Hit
ATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAATGACGGC17970.17675264956844616No Hit
GAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTTAGTAGGTTTACAGT17750.1745887328792387No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT17680.1739002139326727No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT17120.1683920623601446No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16700.1642609486807485No Hit
GCTTACAGCTGCACATTCCCACCAAACTATAATGCGCAAATTCTACTCTG16340.16071999409840904No Hit
GTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACATA16210.15944131605478642No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT15560.15304792583667345No Hit
CCATTAAAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG15370.1511790886959943No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA14800.14557257727395678No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG14630.14390045983229646No Hit
GTACTGGAAGGGACCTGCAAGCTGTAGAGAAAACACAAGGAAGGGTAAGA14440.14203162269161726No Hit
GGCTAATAGATTTCTTAAAGGATGTGATGGAATCAATGAACAAAGAGGAA14420.1418349029925984No Hit
TTGTAGAACCGCTTAGTAGGTTTACAGTACACTATTATAAGGATAAGCAG14020.13790050901222123No Hit
TATTAGCACTCATATGGCATATGGACATGTTGCATACATGATTCCTTGAA13940.13711363021614578No Hit
TTATAATAGTGTACTGTAAACCTACTAAGCGGTTCTACAACCAATACTTT13640.13416283473086285No Hit
AAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACCGCTT13530.13308087638625912No Hit
ATGCTAGCCTATTTACCCGTAATATAAACTAATGCCGTCATTCACATAGT13250.13032680059999507No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA13150.12934320210490077No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC13020.1280645240612782No Hit
CCTTATGACTCACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA12630.1242284899304104No Hit
CGTGAAATATAATGGAGAGAACTAGCATTCAAAGTATTGGTTGTAGAACC12470.12265473233825952No Hit
TATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTTATTCAGTCTCTGT12440.12235965278973124No Hit
CCATAGAAGTTTTTAGATCGAATGACCTAACAGCTAACGAGTCAGGAAGG12320.12117933459561807No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC12130.11931049745493888No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAAATTGCAACACTTCT12130.11931049745493888No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT12080.11881869820739173No Hit
ACTATAATGCGCAAATTCTACTCTGCTTGTAATACACACTGTATGCTAGC12050.11852361865886346No Hit
CATTAAAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA11920.11724494061524086No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC11650.11458922467848624No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCCATTAAAAGAGGCCTTATTTTC11520.11331054663486365No Hit
GTATTGGTTGTAGAACCGCTTAGTAGGTTTACAGTACACTATTATAAGGA11260.11075319054761847No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAAACGAGAAGATCATTT11190.11006467160105246No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG11050.10868763370792042No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG10990.10809747461086383No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT10850.10672043671773182No Hit
AAATAAGGTATATACGTGAAATATAATGGAGAGAACTAGCATTCAAAGTA10660.10485159957705265No Hit
CCCATAATGTTCTCAAATAAAATGGCAAGACTAGGAAAAGGGTACATGTT10650.10475323972754323No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT10560.10386800108195834No Hit
CTTCAGGAATGTGAAGATTCCGTATGTTGTATAAGTTTGGTCCTCCATCT10480.1030811222858829No Hit
GTACACTATTATAAGGATAAGCAGCGCAATGAAGCAGAAAACTATGTGAA10410.10239260333931689No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT10380.1020975237907886No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC10270.10101556544618487No Hit
CTCATATGGCATATGGACATGTTGCATACATGATTCCTTGAAACATAAAA10240.10072048589765657No Hit
TAATACACACTGTATGCTAGCCTATTTACCCGTAATATAAACTAATGCCG10230.10062212604814716No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA100.0070828865145.054721
GCCTAAA100.0070828865145.054721
AAGGTCC151.2291271E-4145.026185
TCCTAGA203.8663263E-4108.748239
TTGGCAC259.380065E-487.01573
GGCACTA259.380065E-487.01575
CTGAGGG1000.087.01574
CCTGAGG1000.087.01573
GTCCTAG259.387399E-486.998588
GTAAGCC259.387399E-486.99858145
CCCTGAG1150.081.9713062
CGAAAGC6050.079.105193
GTAGAAA4500.075.75081
GATATAG8050.073.87881
ATATAGA8200.073.397392
GCCCTGA1300.072.527351
TATAGAC8300.071.6394353
ATAGACA8350.071.210464
ACAGAGC9600.062.6812748
ATAGGGT1350.059.0847364
Produced by FastQC (version 0.11.5)