FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_463_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_463_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899393
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT76640.40349732783052267No Hit
CTCTATGACCGTCTGAAAGGCCATTTGCGTTCGTTTTACGGCTACTTTGC75030.3950209356357531No Hit
GTATAATATGTAGATTGATTTTATTACCCATATAACTCTTAACTCTGCTG74250.3909143605351815No Hit
ATATAAGGCAGCCATCGGGAGCGCTAAAACCTCCACCTCCTAAAATTGCA69360.36516929355852107No Hit
GATAAGGACCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGG55680.29314628410234217No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC53060.27935240363631964No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA51750.2724554634033083No Hit
GCCTTATATTGGGCCCCCTCTCCTCTCCCTCTTGGGTTTACTTATTGCCC46730.24602596724321926No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC44410.23381153873895502No Hit
GTTCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC44170.23254797716954836No Hit
GTTAAGGAGAGGGAGAGGGAGAGGACCTTTAGGTGCTCGACTGTTAAGGA42190.22212359422194355No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG41280.21733258993794333No Hit
GTCTTATTAGCAACCTCCATGGCCTCAGCTGCCTGTTCACTCGATCCAGC40110.21117272728708594No Hit
CCATTATATATGTCATTTCTCTCAATGAAATGAAAGAGAAAGCTTCCGAA39200.20638172300308572No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA38990.20527610662985488No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCAGCTGCCTGTTCACTCGAT38440.20238044469996466No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT38400.20216985110506355No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC37420.19701030802998643No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT36630.19285108453068955No Hit
CTATTGGAACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT36530.19232460054343678No Hit
CATATAGACTGTTGAACACAGATTTTGCCAGTAAGGTTCTGCACACTTTT35950.18927099341737072No Hit
AATAAGGAGAGGGGGCTCGACTGATAAGGACCTTTAGGTGCTCGACCCCA35870.1888498062275685No Hit
AATAAGGAGAGGGGGGCTCGACTGATAAGGAGAGGAGAGGACCTTTAGGT34070.17937309445701863No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT33550.17663537772330423No Hit
CCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGCTCGAC33060.17405560618576565No Hit
ATATAGTGGTATTCCTTCCCCCTCGTGACTCGGGTCTTCAATGCTTAATT33030.17389766098958984No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG32770.17252880262273262No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA32230.16968578909156767No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC32180.1694225470979413No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC32080.16889606311068853No Hit
GCACTGGAGCTAGGATGAGTTCCAATAGTTCTCATTGCATGTACCATCTG32000.1684748759208863No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG31800.16742190794638076No Hit
ATATATGTCATTTCTCTCAATGAAATGAAAGAGAAAGCTTCCGAAGCACA31720.16700072075657857No Hit
CCTTTAGGTGCTCGACCCCAGGTTTACTTATTGGGTGGGGCAATAAGTAA31570.1662109947756994No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT31060.1635259264407103No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT30960.16299944245345752No Hit
CCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGGCTCGA30600.16110410009934753No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC30560.16089350650444642No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA30340.15973524173249032No Hit
ATATAACTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTT30330.15968259333376505No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC29720.15647104101152315No Hit
ATAATATGTAGATTGATTTTATTACCCATATAACTCTTAACTCTGCTGAC29520.1554180730370176No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC29040.15289094989820431No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA28720.15120620113899544No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT27980.14731021963332497No Hit
ATGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAGCTTTCCCTCC27830.14652049365244582No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC27810.14641519685499527No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCAGCTGC27510.14483574489323695No Hit
GATAAGGACCTTTAGGTGCTCGACCCCAGGTTTACTTATTGGGTGGGGCA27160.14299305093785225No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT27080.14257186374805003No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC26900.14162419257099504No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG26700.14057122459648952No Hit
TGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAGCTTTCCCTCCT26430.13914971783090704No Hit
TTCTTGATATATGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAG26010.1369384850844454No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCTGAGGCCATGG25970.13672789148954428No Hit
ACTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTTCTCTC25860.13614875910356625No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC25730.13546432992013763No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC25660.1350957911290607No Hit
CTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTTCTCTCA25570.1346219555405332No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTGGAA25430.13388487795837933No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT24780.13046273204123635No Hit
ATATTGAGGACTGCGATAGGTCAAGTGTCGAGGCCCATGTTCCTATATGT24610.12956770926290662No Hit
CCTCAATATCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT24550.12925181887055495No Hit
CACTTGAACCGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAA24350.1281988508960494No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT23810.12535583736488445No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAG23730.12493465017508225No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTCTCAG23430.12335519821332394No Hit
ATAATAACATATATCAAGAATGACCAACGGGAGTACGCAGGGACCAACCC23310.12272341742862061No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT22920.12067012987833482No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT22890.12051218468215898No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG22730.11966981030255455No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC22580.1188800843216754No Hit
GTATATGTAACACAACAGGGATAGAGAAGCCTAAATTTCTTCCTGATTTG22580.1188800843216754No Hit
CCCTTATGGTCGAGCACCTAAAGGTCCTCTCCTCTCCTTATCAGTCGAGC22000.11582647719560935No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG21980.11572118039815879No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT21790.11472086082237852No Hit
ATATTGGGCCCCCTCTCCTCTCCCTCTTGGGTTTACTTATTGCCCCACCC21240.11182519889248828No Hit
CTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC21190.1115619568988619No Hit
GATAAGGAGAGGAGAGGACCTTTAGGTGCTCGACCATAAGGGAGAGGATG21000.11056163732308164No Hit
GTTAAGGATGCGTAAGCAGGCTCGACTGATAAGGACCCTTTAGGGTGCGT20960.11035104372818053No Hit
GTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTT20850.10977191134220249No Hit
TCTCAGACCAGCACTGGAGCTAGGATGAGTTCCAATAGTTCTCATTGCAT20770.10935072415240027No Hit
GTTTAAGGTCTCCAACATCTTTACAGTCATCAAAGTCCACTTTTTCTGGT20580.10835040457662No Hit
AGACTAGGCAGATGGTACATGCAATGAGAACTATTGGAACTCATCCTAGC20390.10735008500083974No Hit
GTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCC20270.10671830421613643No Hit
ACCATAAGGGAGAGGATGCGCAAGCATGCTCGACTGTTAACCCCCCAACC20250.10661300741868587No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG20240.10656035901996058No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGAT20170.10619182022888365No Hit
CCTAAAGGTTAGGGTTTACTTATTGGGTTGGGGGGTTAACAGTCGAGCAT20140.10603387503270781No Hit
CAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCC20070.10566533624163088No Hit
CCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGCTCGACT20020.1054020942480045No Hit
ACCTTTAGGTGCTCGACCCCAGGTTTACTTATTGGGTGGGGCAATAAGTA19920.10487561026075172No Hit
ATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTT19880.10466501666585061No Hit
GGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTGCCACT19850.10450707146967479No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC19820.10434912627349895No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC19750.10398058748242202No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG19700.10371734548879563No Hit
CAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTG19690.10366469709007035No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG19650.10345410349516926No Hit
CTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCT19520.10276967431174064No Hit
CCCTAAAGGGTCCTTATCAGTCGAGCACCTAAAGGTCCTCTCCTCTTGGG19510.10271702591301538No Hit
GGTTTACTTATTGGGTGGGGCAATAAGTAAACCCAAGAGGAGAGGACCTT19270.10145346434360873No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA19180.10097962875508122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTG100.0070882854145.021043
AGTGAGG100.0070888433145.017236
AGTAGAA3550.0116.443731
CAGGTAC3000.094.263683
GTACTGA3300.087.889226
TGTAGTA259.383629E-487.010335
CCGACTA259.387556E-487.00117145
CGAAAGC6200.085.375293
GCGAAAG6400.082.707312
GAAAGCA6850.077.271944
GGTACTG4000.074.321335
GTAGTAT502.3028897E-672.521981
CTTGTAG300.001934897372.510513
AGGACTA300.001935099572.508615
CCTGGTC502.307217E-672.499078
GTGTTTT851.6916601E-1068.234419
AGGTACT4300.067.449874
GTAGAAA6150.067.204872
GGACAAT4600.066.203516
GCCCTGG554.0601462E-665.916926
Produced by FastQC (version 0.11.5)