FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_463_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_463_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905961
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT76910.40352347188636073No Hit
CTCTATGACCGTCTGAAAGGCCATTTGCGTTCGTTTTACGGCTACTTTGC75280.3949713556573298No Hit
GTATAATATGTAGATTGATTTTATTACCCATATAACTCTTAACTCTGCTG74500.39087893194037027No Hit
ATATAAGGCAGCCATCGGGAGCGCTAAAACCTCCACCTCCTAAAATTGCA69550.36490778142889596No Hit
GATAAGGACCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGG55820.29287063061626134No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC53180.279019350343475No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA51890.27225111111927264No Hit
GCCTTATATTGGGCCCCCTCTCCTCTCCCTCTTGGGTTTACTTATTGCCC46800.24554542301757484No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC44560.2337928215739986No Hit
GTTCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC44310.23248114730574235No Hit
GTTAAGGAGAGGGAGAGGGAGAGGACCTTTAGGTGCTCGACTGTTAAGGA42220.22151555042311988No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG41380.21710832488177878No Hit
GTCTTATTAGCAACCTCCATGGCCTCAGCTGCCTGTTCACTCGATCCAGC40250.2111795571892604No Hit
CCATTATATATGTCATTTCTCTCAATGAAATGAAAGAGAAAGCTTCCGAA39310.20624766194061683No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA39100.20514585555528156No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT38580.20241757307730848No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCAGCTGCCTGTTCACTCGAT38530.20215523822365725No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC37570.19711840903355315No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT36710.19260624955075156No Hit
CTATTGGAACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT36650.19229144772637005No Hit
CATATAGACTGTTGAACACAGATTTTGCCAGTAAGGTTCTGCACACTTTT36170.189773033131318No Hit
AATAAGGAGAGGGGGCTCGACTGATAAGGACCTTTAGGTGCTCGACCCCA35980.18877616068744324No Hit
AATAAGGAGAGGGGGGCTCGACTGATAAGGAGAGGAGAGGACCTTTAGGT34220.17954197383891907No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT33670.17665629044875525No Hit
CCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGCTCGAC33160.17398047494151245No Hit
ATATAGTGGTATTCCTTCCCCCTCGTGACTCGGGTCTTCAATGCTTAATT33110.17371814008786118No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG32900.1726163337025259No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC32330.1696257163709016No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA32280.16936338151725033No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC32180.16883871180994786No Hit
GCACTGGAGCTAGGATGAGTTCCAATAGTTCTCATTGCATGTACCATCTG32080.16831404210264533No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG31940.16757950451242182No Hit
ATATATGTCATTTCTCTCAATGAAATGAAAGAGAAAGCTTCCGAAGCACA31800.1668449669221983No Hit
CCTTTAGGTGCTCGACCCCAGGTTTACTTATTGGGTGGGGCAATAAGTAA31680.1662153632734353No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT31170.1635395477661925No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT31090.1631198120003505No Hit
CCCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGGCTCGA30680.1609686662004102No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC30660.16086373225894968No Hit
ATATAACTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTT30470.15986685981507492No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA30410.1595520579906934No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC29840.1565614406590691No Hit
ATAATATGTAGATTGATTTTATTACCCATATAACTCTTAACTCTGCTGAC29590.15524976639081284No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC29150.15294121967868177No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA28820.1512098096445835No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT28040.1471173859276239No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC27920.1464877822788609No Hit
ATGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAGCTTTCCCTCC27840.14606804651301888No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCAGCTGC27670.14517610801060463No Hit
GATAAGGACCTTTAGGTGCTCGACCCCAGGTTTACTTATTGGGTGGGGCA27250.14297249523993408No Hit
GCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGAT27180.14260522644482232No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC26990.14160835400094757No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG26800.1406114815570728No Hit
TGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAGCTTTCCCTCCT26530.13919487334735603No Hit
TTCTTGATATATGTTATTATTGACCAATAAGATGGCGGAGGGTTTCACAG26110.13699126057668548No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCTGAGGCCATGG26050.13667645875230394No Hit
ACTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTTCTCTC25920.1359943881328107No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC25830.13552218539623842No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC25780.13525985054258718No Hit
CTCTTAACTCTGCTGACTTGATATCCCCATTATATATGTCATTTCTCTCA25720.13494504871820567No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTGGAA25560.13410557718652166No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT24830.1302754883232133No Hit
ATATTGAGGACTGCGATAGGTCAAGTGTCGAGGCCCATGTTCCTATATGT24730.1297508186159108No Hit
CCTCAATATCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT24670.1294360167915293No Hit
CACTTGAACCGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAA24440.12822927646473353No Hit
CTTTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT23860.12518619216237897No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAG23850.12513372519164873No Hit
CCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTCTCAG23580.12371711698193194No Hit
ATAATAACATATATCAAGAATGACCAACGGGAGTACGCAGGGACCAACCC23330.12240544271367568No Hit
GGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCT23020.12077896662103789No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT22930.12030676388446564No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG22820.11972962720643288No Hit
GTATATGTAACACAACAGGGATAGAGAAGCCTAAATTTCTTCCTGATTTG22660.11889015567474887No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC22620.11868028779182785No Hit
CCCTTATGGTCGAGCACCTAAAGGTCCTCTCCTCTCCTTATCAGTCGAGC22090.11589953834312454No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG22030.11558473651874304No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT21850.11464033104559852No Hit
ATATTGGGCCCCCTCTCCTCTCCCTCTTGGGTTTACTTATTGCCCCACCC21260.11154477977251372No Hit
CTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACC21260.11154477977251372No Hit
GATAAGGAGAGGAGAGGACCTTTAGGTGCTCGACCATAAGGGAGAGGATG21090.11065284127009943No Hit
GTTAAGGATGCGTAAGCAGGCTCGACTGATAAGGACCCTTTAGGGTGCGT21040.11039050641644818No Hit
GTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTT20940.10986583670914568No Hit
TCTCAGACCAGCACTGGAGCTAGGATGAGTTCCAATAGTTCTCATTGCAT20850.10939363397257341No Hit
GTTTAAGGTCTCCAACATCTTTACAGTCATCAAAGTCCACTTTTTCTGGT20670.10844922849942891No Hit
AGACTAGGCAGATGGTACATGCAATGAGAACTATTGGAACTCATCCTAGC20440.10724248817263313No Hit
GTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCC20320.1066128845238701No Hit
CCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACGCTCACCGTGCCCAG20290.10645548361167935No Hit
ACCATAAGGGAGAGGATGCGCAAGCATGCTCGACTGTTAACCCCCCAACC20280.10640301664094912No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGAT20220.1060882148165676No Hit
CCTAAAGGTTAGGGTTTACTTATTGGGTTGGGGGGTTAACAGTCGAGCAT20220.1060882148165676No Hit
CAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCC20110.10551107813853483No Hit
CCTTTAGGGTGCGTAAGCACGCTCGACTAATAAGGAGAGGGGGCTCGACT20100.10545861116780458No Hit
GGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTGCCACT19980.10482900751904159No Hit
ATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTT19950.10467160660685082No Hit
ACCTTTAGGTGCTCGACCCCAGGTTTACTTATTGGGTGGGGCAATAAGTA19950.10467160660685082No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC19900.10440927175319956No Hit
TTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCCC19810.1039370690166273No Hit
CAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTG19780.10377966810443656No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG19780.10377966810443656No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG19750.1036222671922458No Hit
CCCTAAAGGGTCCTTATCAGTCGAGCACCTAAAGGTCCTCTCCTCTTGGG19570.10267786171910129No Hit
CTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCT19550.10257292777764078No Hit
GGTTTACTTATTGGGTGGGGCAATAAGTAAACCCAAGAGGAGAGGACCTT19380.10168098927522652No Hit
ATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGAGCCTA19250.10099891865573325No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACC19080.100106980153319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGGCG100.00708839145.020325
TGCTCGC100.007091171145.00131145
AGTAGAA3750.0119.918091
CAGGTAC3100.095.903063
GTACTGA3300.090.088396
GGCCCCG259.388704E-486.9985059
GGTACTG3500.084.940485
CGAAAGC5600.076.3966453
GTAGAAA6050.074.309892
CTTGTAG608.026291E-872.512073
TTAGTAG300.00193473472.512073
GCGAAAG5950.071.9027252
GATTAGA1250.069.611592
GGACTAA1050.069.0573046
GGTCGTT1050.069.046437
TGTAGTA651.397384E-766.932465
GTAGTAT651.397384E-766.932466
CGGATGG554.0627965E-665.90968145
AGGTACT4950.064.453484
GAAAGCA6750.064.453484
Produced by FastQC (version 0.11.5)