FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_420_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_420_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499611
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA83170.5546104956552066No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT78620.5242692938368684No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA67120.44758273979051905No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT63820.42557703297721877No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT61140.4077057316864173No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT49250.32841850319849614No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC42860.28580745273274205No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT40630.2709369296437543No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT39960.2664691043210539No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTTTGTAATGATGACAAGCAA39870.26586894868069116No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG38500.25673324615516957No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT37990.25333236419311406No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA37150.2477309115497286No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC35950.23972883634489212No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG33460.2231245302948565No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT32970.2198570162528816No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA32920.21952359645268008No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT32210.2147890352898185No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG32040.21365540796913332No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA31800.21205499292816604No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT31380.20925426660647325No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT27140.18098026754938448No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT26050.17371171590499138No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT25840.17231135274414497No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC25820.17217798482406438No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT25540.17031083394293586No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC25240.16831031514172676No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT24960.16644316426059824No Hit
CCATTAGAGAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA24930.16624311238047734No Hit
CTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC24420.16284223041842183No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA24410.16277554645838155No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAAGAAAG24230.1615752351776561No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC23610.15744082965515724No Hit
GTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGTAACTGTGT23440.15630720233447207No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA23320.15550699481398844No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA23220.1548401552135854No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG22910.15277295245233596No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA22510.15010559405072382No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT22300.14870523088987744No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT21230.14157004716556496No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA20760.13843590104367065No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC20440.13630201432238093No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA20050.1337013398808091No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA19990.13330123612056727No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC19940.13296781632036575No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTTTGTAATGATGACAAGCAA19810.13210092483984182No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG19640.13096729751915664No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT19330.12890009475790723No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA18620.12416553359504565No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC17790.11863076491170045No Hit
AAATACAACAATAACCTCCTTCAGATTGAATGATTTGAATGGAGCTGACA17640.11763050551109588No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCCTTAGCCT16740.11162894910746854No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC16680.11122884534722673No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA16580.11056200574682369No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA16470.10982848218638033No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA16360.10909495862593699No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG16280.10856148694561457No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC16270.10849480298557425No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT16070.10716112378476818No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT16020.10682770398456666No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT15990.10662765210444575No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG15970.10649428418436513No Hit
CAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATAAGA15960.10642760022432485No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT15900.10602749646408302No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC15710.10476050122331725No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT15430.10289335034218874No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG15430.10289335034218874No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC15370.10249324658194693No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC15160.10109288342110054No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT15120.10082614758093933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC11850.0124.845861
GCACGCA12200.0121.248052
CACGCAC12400.0119.87723
TGGGCTA256.4620926E-6116.013885
CGGTGCA352.7599162E-7103.587272
GGGCTAG301.5990365E-596.6756
GGCTAGG301.599831E-596.665337
CGCACTT16300.090.747055
ACGCACT16400.090.635844
GAAACAC4400.090.635844
AAACACG4550.089.241445
GTAACAA15950.087.737774
CTGGGCT353.435799E-582.867064
CAAGAGG4250.081.881228
TAGTAAC17350.081.0786742
GGTGCAT451.232067E-680.567883
CACTTTC21300.076.923827
GCGGTGC851.8189894E-1276.786691
AACACGT1500.072.5062566
GCTAGGG406.664728E-572.4998
Produced by FastQC (version 0.11.5)