FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_416_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_416_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712466
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT88230.5152219080554008No Hit
CTCCAACACCACGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC71830.419453583312019No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT64560.37700018569711746No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG64110.37437239629867103No Hit
GGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAAAGTC46740.27293972551863804No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTG44860.2619614053651284No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA41570.2427493450964866No Hit
CCTCTGCACTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG40400.2359170926605258No Hit
TCTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG40370.23574190670062936No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC38880.22704100402577335No Hit
TTCCTTGACCGGCTACGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAA36320.21209180211461134No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT35650.2081793156769244No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT35650.2081793156769244No Hit
ACATAATGGACTCCAACACCACGTCAAGCTTTCAGGTAGACTGTTTTCTT33620.1963250657239326No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC32720.1910694869270397No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCTCGCCATTTCTCATTTC32410.18925923200810996No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT32280.18850009284855873No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT32120.18756576772911113No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG32070.18727379112928375No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT30760.17962400421380628No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC29670.17325891433756935No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT28450.16613468530178116No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG27440.16023675798526804No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT27280.15930243286582038No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA26960.15743378262692515No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA26930.15725859666702874No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT26770.15632427154758108No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA26540.15498117918837512No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA26060.15217820383003225No Hit
GTACCTACTTCGCGCTACATCTCTGACATGACCCTCGAGGAAATGTCACG25860.1510102974307227No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT24870.14522916075414052No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGAACTCC24390.14242618539579763No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT24350.14219260411593573No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC23290.1360027001995952No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC22990.1342508406006309No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC22680.13244058568170114No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAACTTA22290.13016316820304755No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATTTTAAAAGAGGAATCCAGC22210.12969600564332373No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC22150.12934563372353086No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAA21860.12765216944453203No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTT21750.12700982092491178No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG21680.12660105368515345No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG21030.12280535788739748No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAGATGTAG20950.12233819532767366No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTATTTCTTGTTC20930.1222214046877427No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG20800.12146226552819152No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT20590.12023596380891649No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC20580.12017756848895103No Hit
GTTCTTATTTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA20400.11912645272957245No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC20400.11912645272957245No Hit
GTCATACTCCTCTGCACTGTCTCCGAAGAAATAAGACCCTTCATTACTCA20270.11836731357002124No Hit
CATCTGTACCTACTTCGCGCTACATCTCTGACATGACCCTCGAGGAAATG20250.11825052293009028No Hit
GTACTATGTTCTTTTCCATGACCGCCTGATCCAATCGCACGCAAAGAGGG20240.1181921276101248No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA20130.11754977909050457No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG20110.11743298845057362No Hit
GTTGAAGATTACACTGAAGTTTGCTTCCAGTACTATGTTCTTTTCCATGA19780.11550594289171287No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACATTCC19730.11521396629188549No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCACGTCAAGCTTTC19560.1142212458524724No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGGATTGGGTGAT19520.11398766457261049No Hit
TGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGAT19520.11398766457261049No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT19270.11252778157347357No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCG19210.1121774096536807No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC18950.11065913133457832No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCC18880.11025036409481997No Hit
CTCCAATACCCTGGAACTAAGAAGCAGATACTGGGCCATAAGGACCAGGA18830.1099583874949926No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGATC18690.10914085301547594No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA18680.10908245769551046No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGATCAGGCGGTCATGGAAAAG18600.10861529513578663No Hit
GAATAAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT18450.10773936533630449No Hit
GTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA18240.10651306361702947No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT18210.10633787765713305No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT18170.10610429637727115No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC18020.10522836657778899No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT17840.1041772508184104No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACATCTCTGAC17790.10388527421858303No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA17590.1027173678192735No Hit
TCATTAAATAAGCTGAAACGAGAAAGTTCTTATTTCTTGTTCTACTTCAA17430.10178304269982588No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT17410.10166625205989491No Hit
ATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA17340.10125748482013658No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTC17240.10067353162048182No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATT17220.10055674098055085No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG17220.10055674098055085No Hit
GTTCTTTTCCATGACCGCCTGATCCAATCGCACGCAAAGAGGGCCTATTA17130.10003118310086156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTA100.0070836907145.052081
TCGCGCC151.2301149E-4145.00125145
CAGGTAC301.1010343E-7120.852033
GAGCGAA750.0106.371531
GTTTTTC259.382134E-487.013453
GCGAAAG5150.084.479082
GTACTGA451.2323435E-680.565666
CGAAAGC5700.076.327593
GTAGAAA11300.071.884221
CTAACAC350.003564752662.152473
TAGAGGG2100.062.1524665
GAATTCG350.003566815362.143394145
AGGGACT350.003567227962.1415829
TTAACAG1750.058.000504145
CAAGGTA4150.057.6494189
TAGATAT4000.056.1961863
GATATTT4050.055.5024075
TGGGCGG400.006047917554.3834085
CTGTAGG553.213948E-452.746211
AGTAGAA9950.052.481161
Produced by FastQC (version 0.11.5)