FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_416_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_416_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718468
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT88570.5154009268720745No Hit
CTCCAACACCACGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC72150.4198507042319089No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT64770.3769054762730525No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG64330.37434505617794456No Hit
GGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAAAGTC46920.27303388832378606No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTG44990.26180295472478976No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTA41740.2428907608404695No Hit
CCTCTGCACTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG40530.23584960557892265No Hit
TCTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG40420.2352095005551456No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC39080.2274118575382259No Hit
TTCCTTGACCGGCTACGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCAA36470.21222391106497182No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTT35770.20815051545911822No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT35740.2079759413617245No Hit
ACATAATGGACTCCAACACCACGTCAAGCTTTCAGGTAGACTGTTTTCTT33750.19639585956794073No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC32860.1912168280119269No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGTTCTCGCCATTTCTCATTTC32480.18900555611160638No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT32390.1884818338194252No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT32210.18743438923506286No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG32170.18720162377187125No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT30870.17963674621814316No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTGATTAGCAAC29740.1730611218829795No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT28510.16590358388983675No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG27570.16043359550483338No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT27370.1592697681888752No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA27060.15746583584914003No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA27030.15729126175174632No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT26870.15636019989897978No Hit
GGTTCATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA26590.15473084165663833No Hit
GGCATAAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA26220.15257776112211574No Hit
GTACCTACTTCGCGCTACATCTCTGACATGACCCTCGAGGAAATGTCACG25940.1509484028797743No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT24960.1452456490315793No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGAACTCC24460.14233608074168388No Hit
CTATTGGGACTCATCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTT24410.14204512391269433No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC23410.1362259873329035No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC23040.13407290679838088No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC22740.13232716582444365No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAACTTA22410.13040685075311265No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATTTTAAAAGAGGAATCCAGC22280.12965036299773985No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC22250.1294757889003461No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGACCGGCTACGCCGAGATCAAA21890.12738089973162142No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTT21820.12697356017103606No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG21720.12639164651305698No Hit
TCCTAGTTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG21090.12272559046778875No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGTTCTTATTTCTTGTTC21020.12231825090720339No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAGATGTAG20960.12196910271241594No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATACATTTGAAAAAAAGACG20850.12132899768863895No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT20640.12010697900688286No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC20620.11999059627528705No Hit
GTTCTTATTTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA20470.11911772578831843No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC20440.1189431516909247No Hit
CATCTGTACCTACTTCGCGCTACATCTCTGACATGACCCTCGAGGAAATG20350.11841942939874353No Hit
GTCATACTCCTCTGCACTGTCTCCGAAGAAATAAGACCCTTCATTACTCA20340.11836123803294563No Hit
GTACTATGTTCTTTTCCATGACCGCCTGATCCAATCGCACGCAAAGAGGG20310.1181866639355519No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA20200.1175465589117749No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG20130.11713921935118955No Hit
GTTGAAGATTACACTGAAGTTTGCTTCCAGTACTATGTTCTTTTCCATGA19840.1154516697430502No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTTAAAAGAGAAATAACATTCC19800.11521890427985858No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCACGTCAAGCTTTC19610.11411326832969833No Hit
TGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGAT19590.11399688559810249No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGGATTGGGTGAT19570.11388050286650668No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT19340.11254210145315478No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCG19290.11225114462416524No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC19030.11073816911341963No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCC18920.11009806408964264No Hit
CTCCAATACCCTGGAACTAAGAAGCAGATACTGGGCCATAAGGACCAGGA18900.10998168135804681No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGATC18810.10945795906586564No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA18770.109225193602674No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGATCAGGCGGTCATGGAAAAG18650.1085268972130991No Hit
GAATAAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGTTT18500.10765402672613049No Hit
GTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCTGTCCCA18320.10660658214176814No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT18270.10631562531277859No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT18260.10625743394698069No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC18040.1049772238994267No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT17890.10410435341245806No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACATCTCTGAC17830.10375520521767062No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA17640.10264956926751036No Hit
TCATTAAATAAGCTGAAACGAGAAAGTTCTTATTTCTTGTTCTACTTCAA17480.10171850741474382No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT17430.1014275505857543No Hit
ATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA17380.10113659375676474No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTC17300.10067106283038149No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATT17260.10043829736718984No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG17260.10043829736718984No Hit
GTTCTTTTCCATGACCGCCTGATCCAATCGCACGCAAAGAGGGCCTATTA17210.1001473405382003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG100.0070868866145.030214
TCGCTCC100.0070868866145.030215
GTTATAG100.0070868866145.030213
CATCGGG100.0070912037145.00067145
GAGCGAA650.0133.897431
CTGTAGG256.4539054E-6116.044431
AGGACGT256.4623164E-6116.014046
CTAACAC301.5981339E-596.6868063
GCGAAAG5400.088.629572
CGAAAGC5800.082.517193
GTAGAAA10500.072.527771
TAGAGGG1950.070.655755
CAATTCG1206.002665E-1154.373679
AGGGTGA6500.052.429426
GTGTAGG604.9367483E-448.351851
GCACTGT13600.047.9837466
AGTAGAA10050.047.6301771
CAAGGTA4250.046.068424
GGGAGGT1002.7313763E-643.5090644
CATACTC6850.042.3445853
Produced by FastQC (version 0.11.5)