FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_412_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_412_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4898
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC260.5308289097590854No Hit
GGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGT180.36749693752552065No Hit
GAGGAGAGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG170.34708044099632507No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT160.32666394446712943No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG160.32666394446712943No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT160.32666394446712943No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT160.32666394446712943No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG150.30624744793793385No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG140.28583095140873827No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCACAAAT140.28583095140873827No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG140.28583095140873827No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC130.2654144548795427No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT130.2654144548795427No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG130.2654144548795427No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG130.2654144548795427No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC120.2449979583503471No Hit
CTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCA120.2449979583503471No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGG110.2245814618211515No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC110.2245814618211515No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG110.2245814618211515No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAAAATGCC100.2041649652919559No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC100.2041649652919559No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG100.2041649652919559No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC100.2041649652919559No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT100.2041649652919559No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT100.2041649652919559No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA90.18374846876276033No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC90.18374846876276033No Hit
GGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAAT90.18374846876276033No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC90.18374846876276033No Hit
ATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGCTCCTG90.18374846876276033No Hit
GTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAG90.18374846876276033No Hit
ACATCCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTC90.18374846876276033No Hit
AGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGC90.18374846876276033No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC90.18374846876276033No Hit
CCTTGGCCCCATGGAACGTTATTTCTCTTTTGAGCTTCTTGTATAGTTTA80.16333197223356472No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC80.16333197223356472No Hit
AAAGCAGGAAGACGGAAAACAAACCTGTATGGGTTCATTATAAAAGGAAG80.16333197223356472No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGAACCCC80.16333197223356472No Hit
GCTTTACCCCATCTATTTTTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT80.16333197223356472No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT80.16333197223356472No Hit
TGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTGCCACTTGT80.16333197223356472No Hit
GGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTCCTTAAA80.16333197223356472No Hit
CTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCAT80.16333197223356472No Hit
ATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGACGAGAGGATCA80.16333197223356472No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC80.16333197223356472No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT70.14291547570436913No Hit
GATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGT70.14291547570436913No Hit
GCTATGACGAGAGGATCACTTGAACCGCTGCATCTGCACTCCCATTCGCT70.14291547570436913No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA70.14291547570436913No Hit
CCTTATTTCCACAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC70.14291547570436913No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATAAGCA70.14291547570436913No Hit
CTAGTAGTTCTGCTATATACATTTACAACCGCAAATGCAGACACATTATG70.14291547570436913No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT70.14291547570436913No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT70.14291547570436913No Hit
GTGTAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT70.14291547570436913No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT70.14291547570436913No Hit
CACTTGAACCGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAA70.14291547570436913No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC70.14291547570436913No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT70.14291547570436913No Hit
CAGCATCGGTCTCACAGACAAATGGCTACTACCACCAATCCACTAATCAG70.14291547570436913No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT70.14291547570436913No Hit
CTGCAGCGTAGACGCTTTATCCAAAATGCCCTAAATGGAAATGGGGACCC70.14291547570436913No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA70.14291547570436913No Hit
GTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATT70.14291547570436913No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCGTCGGAGGACTTGAG70.14291547570436913No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC70.14291547570436913No Hit
ACTAAGGGCTTTCACTGAGGAGAGAGCAATAGTTGGAGAAATTTCACCAT70.14291547570436913No Hit
GACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACG70.14291547570436913No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAGATCCTTGTTGATTCCAGCTTT70.14291547570436913No Hit
CATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGA70.14291547570436913No Hit
TCCTTGTTGATTCCAGCTTTACCCCATCTATTTTTTCTCTGTTTAATTTT60.12249897917517355No Hit
GGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCA60.12249897917517355No Hit
GTGAATGCCCCACAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG60.12249897917517355No Hit
CACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAAAATG60.12249897917517355No Hit
CATCCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCA60.12249897917517355No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA60.12249897917517355No Hit
CTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCT60.12249897917517355No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT60.12249897917517355No Hit
ATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG60.12249897917517355No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG60.12249897917517355No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGACGAGAG60.12249897917517355No Hit
CCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTCCTTAAAAGGAAGA60.12249897917517355No Hit
CAGTAACACACTCTGTTAATCTTCTGGAAGACAAGCATAACGGAAAACTA60.12249897917517355No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGC60.12249897917517355No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC60.12249897917517355No Hit
ACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTT60.12249897917517355No Hit
GCAGAGGCCATGGAGGTTGCTAATAAGACTAGGCAGATGGTACATGCAAT60.12249897917517355No Hit
ATCTAGAGGCCTATTCGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGA60.12249897917517355No Hit
GTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCAGCCGACC60.12249897917517355No Hit
GCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTCCTTAAAAGGAAG60.12249897917517355No Hit
CCTCAAAGCCGAGATCGCACAGAGACTGGAAAGTGTCTTTGCAGGAAAGA60.12249897917517355No Hit
CCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTG60.12249897917517355No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT60.12249897917517355No Hit
GACCAGCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACC60.12249897917517355No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC60.12249897917517355No Hit
CGCCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGC60.12249897917517355No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT60.12249897917517355No Hit
GCTGAAACGAGAAAGCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGT60.12249897917517355No Hit
AGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTG60.12249897917517355No Hit
GACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAA60.12249897917517355No Hit
CATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACTACCAGTACC60.12249897917517355No Hit
GCATGGGCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCT60.12249897917517355No Hit
GTTGTATTGCAATCGTGGACTGGTGTATCTGAAATGATAATACCAGATCC50.10208248264597795No Hit
TCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACC50.10208248264597795No Hit
GAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGT50.10208248264597795No Hit
CATATGCAGTGGGGTTTTCGGAGACAATCCACGCCCTAATGATAAGACAG50.10208248264597795No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG50.10208248264597795No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAATGTAA50.10208248264597795No Hit
GTGATAACCGTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGA50.10208248264597795No Hit
CCATCCATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATG50.10208248264597795No Hit
CTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGA50.10208248264597795No Hit
CTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGA50.10208248264597795No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC50.10208248264597795No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG50.10208248264597795No Hit
TGTCAGAAATGTAACGCGAAGTAGGTACAGATGCAATTGTCATTCTAAGT50.10208248264597795No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTTATG50.10208248264597795No Hit
AGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTGTTCAC50.10208248264597795No Hit
CTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCA50.10208248264597795No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGAGAGCAATAGTTGGAGAA50.10208248264597795No Hit
GTCTTATACAATCCAGTCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG50.10208248264597795No Hit
GGGTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGAACC50.10208248264597795No Hit
ACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACC50.10208248264597795No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG50.10208248264597795No Hit
GTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCC50.10208248264597795No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA50.10208248264597795No Hit
TCTCAGACCAGCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCAT50.10208248264597795No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC50.10208248264597795No Hit
ACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAG50.10208248264597795No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC50.10208248264597795No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA50.10208248264597795No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA50.10208248264597795No Hit
ATTCTGCATTATATGTCCAAATGTCCAGAAATCCATCATCAACTTTTTTA50.10208248264597795No Hit
GTAGAAGGCCCTCTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTT50.10208248264597795No Hit
CATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACC50.10208248264597795No Hit
ATTCGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGACAGGGATGGTAG50.10208248264597795No Hit
GGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATC50.10208248264597795No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT50.10208248264597795No Hit
CTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCA50.10208248264597795No Hit
GCAGTAGTAGATGGATGGTGAATGCCCCACAGCACGAGGACTTCTTTCCC50.10208248264597795No Hit
GGACTGCAGCGTAGACGCTTTATCCAAAATGCCCTAAATGGAAATGGGGA50.10208248264597795No Hit
CAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTG50.10208248264597795No Hit
CCGTGTTACCATTCCACTCAAGTCCTCCGACGAGGACCCCAACTGCATTT50.10208248264597795No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGAC50.10208248264597795No Hit
TTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTT50.10208248264597795No Hit
CCATGGGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCA50.10208248264597795No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA50.10208248264597795No Hit
TCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGCCACCTGTTCCATAGC50.10208248264597795No Hit
GGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCAGAGGCCATGG50.10208248264597795No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG50.10208248264597795No Hit
TTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCC50.10208248264597795No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTT50.10208248264597795No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA50.10208248264597795No Hit
ATGTAACATTGCTGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCT50.10208248264597795No Hit
GTAACACACTCTGTTAATCTTCTGGAAGACAAGCATAACGGAAAACTATG50.10208248264597795No Hit
GTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGTAATCTTGTCA50.10208248264597795No Hit
GGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACA50.10208248264597795No Hit
AGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAATGTA50.10208248264597795No Hit
ATGTAGGACCATGAACTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC50.10208248264597795No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATT50.10208248264597795No Hit
GAACATAGTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGA50.10208248264597795No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCATA100.00687628144.999986
GCACCCC100.00687628144.99998145
Produced by FastQC (version 0.11.5)