FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_412_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_412_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5074
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC260.5124162396531337No Hit
GAGGAGAGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG180.35474970437524633No Hit
GGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGT180.35474970437524633No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT160.31533307055577453No Hit
CTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG160.31533307055577453No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT160.31533307055577453No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT160.31533307055577453No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG150.29562475364603863No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG140.27591643673630273No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCACAAAT140.27591643673630273No Hit
GTCCTACATTGTGGAAACATCTAATTCAGACAATGGAACGTGTTACCCAG140.27591643673630273No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC130.2562081198265668No Hit
GGGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGC130.2562081198265668No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT130.2562081198265668No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG130.2562081198265668No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG130.2562081198265668No Hit
CTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGCAGCA120.2364998029168309No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGG110.21679148600709497No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC110.21679148600709497No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG110.21679148600709497No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT110.21679148600709497No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAAAATGCC100.1970831690973591No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC100.1970831690973591No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG100.1970831690973591No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGC100.1970831690973591No Hit
GTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACT100.1970831690973591No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA90.17737485218762317No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC90.17737485218762317No Hit
GGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAAT90.17737485218762317No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAGCTGATTGCCCC90.17737485218762317No Hit
ATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGCTCCTG90.17737485218762317No Hit
GTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAG90.17737485218762317No Hit
ACATCCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTC90.17737485218762317No Hit
AGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAACAGGC90.17737485218762317No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC90.17737485218762317No Hit
CCTTGGCCCCATGGAACGTTATTTCTCTTTTGAGCTTCTTGTATAGTTTA80.15766653527788727No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC80.15766653527788727No Hit
AAAGCAGGAAGACGGAAAACAAACCTGTATGGGTTCATTATAAAAGGAAG80.15766653527788727No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGAACCCC80.15766653527788727No Hit
GCTTTACCCCATCTATTTTTTCTCTGTTTAATTTTGCTTCCTCTGAGTAT80.15766653527788727No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGGCCACTT80.15766653527788727No Hit
TGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTTTGTGCCACTTGT80.15766653527788727No Hit
GGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTCCTTAAA80.15766653527788727No Hit
CTGCACTCCCATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCAT80.15766653527788727No Hit
ATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGACGAGAGGATCA80.15766653527788727No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC80.15766653527788727No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT70.13795821836815136No Hit
GATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGT70.13795821836815136No Hit
GCTATGACGAGAGGATCACTTGAACCGCTGCATCTGCACTCCCATTCGCT70.13795821836815136No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA70.13795821836815136No Hit
CCTTATTTCCACAAATTTCTGTCCCAATTGTTCCCGCCACTTCTCATTTC70.13795821836815136No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATAAGCA70.13795821836815136No Hit
CTAGTAGTTCTGCTATATACATTTACAACCGCAAATGCAGACACATTATG70.13795821836815136No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT70.13795821836815136No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAT70.13795821836815136No Hit
GTGTAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT70.13795821836815136No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT70.13795821836815136No Hit
CACTTGAACCGCTGCATCTGCACTCCCATTCGCTTCTGGTAGGCCTGCAA70.13795821836815136No Hit
CCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGC70.13795821836815136No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT70.13795821836815136No Hit
CAGCATCGGTCTCACAGACAAATGGCTACTACCACCAATCCACTAATCAG70.13795821836815136No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT70.13795821836815136No Hit
CTGCAGCGTAGACGCTTTATCCAAAATGCCCTAAATGGAAATGGGGACCC70.13795821836815136No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA70.13795821836815136No Hit
GTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATT70.13795821836815136No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCGTCGGAGGACTTGAG70.13795821836815136No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC70.13795821836815136No Hit
ACTAAGGGCTTTCACTGAGGAGAGAGCAATAGTTGGAGAAATTTCACCAT70.13795821836815136No Hit
GACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTGTTCACG70.13795821836815136No Hit
CAGTTGAATAGATCGCCAAAATCTGGTAGATCCTTGTTGATTCCAGCTTT70.13795821836815136No Hit
CATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGA70.13795821836815136No Hit
TCCTTGTTGATTCCAGCTTTACCCCATCTATTTTTTCTCTGTTTAATTTT60.11824990145841545No Hit
GGCCAAGGAGGTGTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCA60.11824990145841545No Hit
GTGAATGCCCCACAGCACGAGGACTTCTTTCCCTTTATCATTAATGTAGG60.11824990145841545No Hit
CACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTATCCAAAATG60.11824990145841545No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACC60.11824990145841545No Hit
CATCCACAGCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCA60.11824990145841545No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA60.11824990145841545No Hit
CTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCT60.11824990145841545No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT60.11824990145841545No Hit
ATCTACTACTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCATATG60.11824990145841545No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAG60.11824990145841545No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCTATGACGAGAG60.11824990145841545No Hit
ACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAG60.11824990145841545No Hit
CCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTCCTTAAAAGGAAGA60.11824990145841545No Hit
CAGTAACACACTCTGTTAATCTTCTGGAAGACAAGCATAACGGAAAACTA60.11824990145841545No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGC60.11824990145841545No Hit
GCACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCC60.11824990145841545No Hit
ACTCTGCTGCTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTT60.11824990145841545No Hit
GCAGAGGCCATGGAGGTTGCTAATAAGACTAGGCAGATGGTACATGCAAT60.11824990145841545No Hit
ATCTAGAGGCCTATTCGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGA60.11824990145841545No Hit
GTACGGTTATCACCATCAAAATGAGCAGGGGTCAGGATATGCAGCCGACC60.11824990145841545No Hit
GCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTCCTTAAAAGGAAG60.11824990145841545No Hit
CCTCAAAGCCGAGATCGCACAGAGACTGGAAAGTGTCTTTGCAGGAAAGA60.11824990145841545No Hit
CCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTG60.11824990145841545No Hit
GTACTGGAAGCAAACTTCAGTGTAATCTTCAACCGATTAGAGACCTTGAT60.11824990145841545No Hit
GACCAGCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACC60.11824990145841545No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC60.11824990145841545No Hit
CGCCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGC60.11824990145841545No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTACACTGAAGTTTGCTTCCAGT60.11824990145841545No Hit
GCTGAAACGAGAAAGCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGT60.11824990145841545No Hit
AGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGATTTGTG60.11824990145841545No Hit
GGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACA60.11824990145841545No Hit
GACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTATTTAAA60.11824990145841545No Hit
CATTAGAGCACATCCAGAAGCTGATTGCCCCCAGGGAGACTACCAGTACC60.11824990145841545No Hit
GCATGGGCCTCATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCT60.11824990145841545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCGA100.0068847653144.999982
TCGATTG100.0068847653144.999985
TTACTCT100.0068847653144.999986
CTCCTCG100.0068847653144.999981
AGTAGAT100.0068847653144.999983
TACGGTT100.0068847653144.999982
CCTCGAT100.0068847653144.999983
TTATCAC100.0068847653144.999987
ACTCTAG100.0068847653144.999988
ATTGCTT100.0068847653144.999988
ATCACCA100.0068847653144.999989
ACGGTTA100.0068847653144.999983
CGGTTAT100.0068847653144.999984
TACTCTA100.0068847653144.999987
AGATTTT100.0068847653144.99998145
GTACGGT100.0068847653144.999981
CTCGATT100.0068847653144.999984
Produced by FastQC (version 0.11.5)