FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_411_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_411_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12115019
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA710050.5860907027880022No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT614180.506957521073636No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT546560.4511425033671016No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAA524560.4329832252017104No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT439940.36313603800373734No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC437880.3614356692300689No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT381110.3145764773460116No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT313810.25902559459461016No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG308170.2543702160103917No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA305120.25185267971928066No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT278030.22949200492380575No Hit
CTACTATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAAT268940.22198892135456No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT263310.2173417969876894No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC244660.20194768163384638No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG241370.1992320441263856No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA236470.1951874776259121No Hit
ACCTAAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAACGCTCTGT233060.19237278951027645No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA227720.18796503744649512No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT223680.18463033363794146No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT216420.17863777184336235No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC214400.17697041993908555No Hit
ACTATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAATGC212490.17539386442563565No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG209500.17292585343861203No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT202460.16711488442568684No Hit
ATATTGTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAATGCAAG200230.16527419395710397No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA199490.164663381873359No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC194700.16070961176371246No Hit
ATACTAAATGGAATGAATGCTTAAATCCACGAATCTTTTTGGCTATGACT191480.15805175377768702No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT191330.15792794051746845No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG187650.15489038853343937No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA185820.15337986675877274No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT184420.15222427633006602No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTAAAAAGGCTGAA183180.15120075337892577No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA182260.1504413653829185No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA181880.1501277051236981No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT171450.1415185564298331No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC170090.140395982870518No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT169120.1395953237877712No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT168830.13935595148468194No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT163120.13464279337902813No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG159750.13186112213278411No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA159680.13180334261134877No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT155650.128476893020143No Hit
GCACAGAGCGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTTAG154730.12771750502413576No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT154180.12726352307000097No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT152690.1260336446851631No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA149330.12326022765626699No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA147540.12178272275099196No Hit
GAATAATTTCCAGGATTTAGGTACATGACCATGAGACAATATAGTAGCGC146190.12066840340902478No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA144090.1189350177659647No Hit
GGATTTAGGTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATG140080.11562507660945476No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT140000.11555904287067152No Hit
GTTGTTCCCATTCCCGATAAGGGCTCTGTGATGAATCTTCTTTTTCTTTC137400.11341294636021619No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT135850.11213354267129089No Hit
AGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGC135540.11187766193350585No Hit
GTTAGATACAAATCCATCTCTATAGAACATGCTTGTAAATTCAAACATTC135380.11174559445593936No Hit
CAATTGCCTACCTGCTTTCATTGACAGAAGATGGAGAAGGCAAAGCAGAA134180.11075508837419075No Hit
GTCTCATGGTCATGTACCTAAATCCTGGAAATTATTCAATGCAAGTAAAA134140.11072207150479912No Hit
GATTATAGGTACACATACAAATGCCACAGAGGAGATTCCAAAGTGGAAGG132780.10959949794548403No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA132730.10955822685874451No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC131980.10893916055765161No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC130150.10742863878298499No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA129730.10708196165437296No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG129160.10661147126554238No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG129070.10653718330941124No Hit
GATTTAGGTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGG128210.1058273206174914No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC127800.10548889770622728No Hit
GTCATGTACCTAAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC127470.10521650853374642No Hit
GATTCAGGTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGG126430.10435806992956428No Hit
GATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTTACTTGTTATC125420.10352439397742588No Hit
GTACATGACCATGAGACAATATAGTAGCGCTGAGCTTTCATGGCCTTCTG124950.10313644576207433No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT124920.1031116831100306No Hit
ATATGGAACTTCTGGTAGCTCATGGTTGTAAATGTGAGGCCCTATTGGTC123320.1017910083343658No Hit
AAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACT123120.1016259239874077No Hit
GTTCTTTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTC123110.1016176697700598No Hit
GTGATGAATCTTCTTTTTCTTTCCTGGTCTTTGGGTTTTAAAAAGCAGAT121670.10042906247196146No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT121630.10039604560256983No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT121320.10014016486478478No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGC100.0070918016145.00018145
AGCACGC57200.0122.613271
GCACGCA58500.0120.235662
CACGCAC58950.0119.317843
GAGCAGA7150.0110.5675051
GAAACAC22250.0108.522784
AACACGT6850.0101.616876
AAACACG24050.0100.098985
ACACGTG7050.098.72197
AACACGA20850.082.419026
CGCACTT89200.081.1282965
ACGCACT89400.080.94684
ACAACGC29300.078.684818
CAACGCA29850.077.235019
ACACGAG22550.076.1961757
ACGTGCC5050.076.087959
AACAACG31550.073.532947
GAGCACG300.001933275772.528231
ACGAGCC13100.070.8386159
CACGTGC10050.069.252678
Produced by FastQC (version 0.11.5)