FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_274_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_274_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562282
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC59870.38322146705908405No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT52110.3335505369709182No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA42360.2711418297080809No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA34820.22287909609148668No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA33680.21558207801152415No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA33560.2148139708452123No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT29200.186906077135882No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA28390.18172135376327705No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT27120.17359221958647672No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT26650.1705837998517553No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC26480.16949564803281353No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA25670.1643109246602086No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC25250.16162254957811714No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT24420.1563098083444602No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA23490.15035697780554344No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA23250.14882076347291975No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC22500.1440200936834707No Hit
AGATTATACTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA21740.1391554149634957No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG20550.13153835223090327No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA19690.12603358420566838No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT19670.1259055663446164No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT19520.1249454323867266No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT19490.12475340559514864No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT18310.11720035179308218No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG17840.11419193205836077No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA17530.11220765521205518No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTACAAAACCAAA17530.11220765521205518No Hit
TGTTTATTCCCAATAATTTACATGTTCGGTAAAAGTCGTTTATTCCTTCC17400.11137553911521735No Hit
GATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAAACCATTCTTC16990.10875117296365189No Hit
CTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG16550.10593478002050846No Hit
CTACAATAAAGGAAAATACAGGGCTTAACTTTCCTTATAGACAAATGGAG16540.10587077108998248No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT16530.10580676215945649No Hit
ATCTTATGCATGGTATTTTCTCAAGACACTAGGATGTTCCAAGGGGTGAG16260.10407852103525483No Hit
TTCTAATGGTATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC16070.10286235135526108No Hit
ATTAGATACAAATCCATCTCTGTAGAACATGCTTGTAAATTCAAACATTC15880.10164618167526733No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT15850.10145415488368938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCAGA100.0070896177145.011116
AGCACGC2450.0121.3824541
GCACGCA2450.0121.355252
CACGCAC2500.0118.928143
GTAACAA14700.0114.934814
GAAACAC5800.0110.018994
AAACACG5850.0109.078665
CGGTGCA203.8628303E-4108.7757342
ACCCACA203.8667675E-4108.747899
AACACGT1750.0103.579356
TAGAAAC6350.0101.6382142
ACACGTG1800.0100.692497
CGCACTT3100.098.242765
ACGCACT3200.095.1726764
GAGCAGA502.2622771E-887.040091
TCTACTA703.092282E-1182.8714455
GAAGCGG1150.081.994291
AACACGA5600.081.568746
TAGTAAC21150.081.260362
ACGTGCA2550.079.6266862
Produced by FastQC (version 0.11.5)