FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_274_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_274_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570602
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC60100.38265582241713686No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT52440.3338847142687963No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA42550.27091522868301454No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA34970.22265347936651042No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA33820.21533144615886135No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA33700.21456740791110668No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT29390.18712570084591768No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA28540.18171376325765534No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT27250.17350035209429251No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT26800.17063520866521245No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC26620.16948915129358044No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA25820.16439556297521588No Hit
CCTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC25410.16178509896205404No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT24540.1562458216658326No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA23590.1501971855377747No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA23400.1489874583121631No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC22560.14363919057788033No Hit
AGATTATACTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA21860.13918230079931135No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG20590.1310962293439076No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA19790.12600264102554307No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT19740.1256842917556453No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT19650.12511126306982928No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT19610.12485658365391104No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT18390.11708886146840512No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG17910.11403270847738638No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA17610.11212261285799968No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTACAAAACCAAA17560.11180426358810189No Hit
TGTTTATTCCCAATAATTTACATGTTCGGTAAAAGTCGTTTATTCCTTCC17490.11135857461024498No Hit
GATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAAACCATTCTTC17040.10849343118116493No Hit
CTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG16670.10613764658392133No Hit
CTACAATAAAGGAAAATACAGGGCTTAACTTTCCTTATAGACAAATGGAG16660.10607397672994176No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT16610.105755627460044No Hit
ATCTTATGCATGGTATTTTCTCAAGACACTAGGATGTTCCAAGGGGTGAG16380.10429122081851416No Hit
TTCTAATGGTATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC16120.10263580461504569No Hit
ATTAGATACAAATCCATCTCTGTAGAACATGCTTGTAAATTCAAACATTC15940.10148974724341368No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT15920.10136240753545456No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTAT100.0070843394145.047262
GTAGCCC100.0070843394145.047263
CGGTGCA100.0070843394145.047262
GACTAGT100.0070843394145.047263
CACGCAC2100.0124.3262253
GCACGCA2150.0121.434922
GTAACAA14500.0117.5382844
AGCACGC2250.0116.059991
GAGCAGA501.8189894E-12116.059981
GAAACAC5050.0113.4528054
TAGAAAC5200.0111.5748142
CGCACTT2350.0111.100025
ACGCACT2400.0108.7854464
AAACACG5350.0107.090975
CGGAGCA406.107239E-790.654532
AACACGT1650.083.5067446
TAGTAAC21150.082.982122
CAACGCA353.438304E-582.8551259
GGAGCAT451.2308283E-680.581813
AACACGA5450.079.837446
Produced by FastQC (version 0.11.5)