FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_268_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_268_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322120
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAATAGTGATCAGGCAGAAAAGAAAAATGGAACATCTAAAAATGTAT94070.7115087889147733No Hit
TTATAGAACTGCAGGATTCACAAGTAGTATTAGAGACATTCCACACTGCA67380.5096360390887362No Hit
GTCCTAGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGCGCAGGC55570.42030980546395186No Hit
ATCTTAGTGGGGGAAAGGCTGCTGCTCGTGCAGGTCTGGGTCCTGTCCAT40890.30927601125465165No Hit
ATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTG40350.30519166187638036No Hit
GTATTAGAGACATTCCACACTGCACACTCCAGTCATATCTCTTAACAAAA40350.30519166187638036No Hit
GTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGAGAAAGTGAACCAAC39270.29702296311983784No Hit
CTCCAGTCATATCTCTTAACAAAATGACATAAATTGACAATGTTCACTAA39170.2962666021238617No Hit
GGTCTAAAGAACGCACTTGAAAGTTGCAAAATTACTTGCCAATGTTTGGG36190.27372704444377216No Hit
GGTCTGGGTCCTGTCCATCAGGATTTTCACAGACTTGGTGTTCTTCATGA35940.2718361419538317No Hit
GTATATGGTATTGGGGGCAGGGAGAACCAGAAACCCTCAAAGAGAACCAA35440.2680543369739509No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCTGGGGATAACAG33420.2527758448552325No Hit
GGGTATGGCTGTTCGCCATTTAAAGAGGTACGTGAGCTGGGTTTAAAACG32010.24211115481196865No Hit
GTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTGATGTGCCTTTTC30540.2309926481711191No Hit
GTGGAGGAGAAAGTGAACCAACTGAAGGAGGAAGTTCGGCTGCAGTACGA28300.21405016186125314No Hit
GCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGAGAAAGTGAACC28240.21359634526366744No Hit
CTACTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAAGAACTCTT27820.21041962908056755No Hit
CTCTAATACTACTTGTGAATCCTGCAGTTCTATAATCATAAACAAAAATT27520.20815054609263908No Hit
GTATTGGGGGCAGGGAGAACCAGAAACCCTCAAAGAGAACCAATTTGTAG26430.19990621123649896No Hit
TGACTGGAGTGTGCAGTGTGGAATGTCTCTAATACTACTTGTGAATCCTG26100.19741021994977762No Hit
TTCTAGCATTTTCCGCAGCTGCTTCTCCTTCTTGGCCACTTCGTTCAGGA25040.18939279339243034No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA24780.18742625480289232No Hit
AAGCAGGACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCT24440.18485462741657338No Hit
ATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGA23790.17993828094272835No Hit
GTACGAGAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACAGTGG23770.17978700874353312No Hit
GTTCTATAATCATAAACAAAAATTACTTAGTTTCGTTAAGCTAAGATTGT23300.17623211206244516No Hit
GGCTATGACTTGTCGCGTCGCTGACTCGATGGTCAACGCCGCCAATATGG23100.17471939007049284No Hit
ACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAG22600.17093758509061205No Hit
GTGTTAATACTTGGGCATTAATCTTCAGGCACCTCCAGTGAGGCAAATGA21100.15959217015096966No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGAGGTACGTGAGCTGGGTT21080.15944089795177444No Hit
GACTTGGTGTTCTTCATGAAGCTGACATCCTCCGTCTTATCAAAGAGCAG20970.15860890085620066No Hit
GTGTGGAATGTCTCTAATACTACTTGTGAATCCTGCAGTTCTATAATCAT20910.15815508425861494No Hit
CTATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACAT19960.15096965479684143No Hit
GGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACGGAT19950.15089401869724384No Hit
GCCTATCAACGTCCTGGTCTCGAACGACCCTTCAGGGAGATCAAGTCTCC19360.14643148882098447No Hit
TTCTTGGCCACTTCGTTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTT18720.14159077844673706No Hit
ATATGAGAGTTTCCTCCAGAGCCATCTTCCAAAGTGGCAGACTTGGCCAA18390.13909478716001572No Hit
CTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAAGAACTCTTCCT18380.13901915106041812No Hit
GTTCTGGACTACAGCCTTGTCTTATGGTCAAATTGATACCCTTAATAAGA18380.13901915106041812No Hit
AACTATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAAC18240.1379602456660515No Hit
GGCAATTAGTACTGGTTAGCTGAACGCATTACTGCGCTTCCACACCCAGC18130.1371282485704777No Hit
CTCCTAGGGTGAGAGGCTTAGCCATCCCTGACCCTGGCAGTGCACTGGTA17660.13357335188938976No Hit
ATTCCACACTGCACACTCCAGTCATATCTCTTAACAAAATGACATAAATT17640.13342207969019454No Hit
GTTCTAAAGTTCTTTGCTCCTTGATGTCGAAGTTAACACAAACACAATCT17280.13069918010468035No Hit
GTGTGTATCAGGCACAAGGCAGACCCAGCCGGAAACATACCGGTTATAGG16880.12767373612077573No Hit
TAGCTACCCTGCGATGCCTCTGGCGAGACAACAGGTACACCAGCGGTACG16880.12767373612077573No Hit
GTTTGAAATTGATCCCTTATTTTACATGAAATAAAAACAATTTCTGTTGC16710.12638792242761626No Hit
TCTAAAAATGTATGTGCTAACTATATCATCCAGTGTGCAGTGTTGTGTAT16650.12593410583003056No Hit
AGCTAAGATTGTGTTTGTGTTAACTTCGACATCAAGGAGCAAAGAACTTT16490.1247239282364687No Hit
GTACATAGCTACCCTGCGATGCCTCTGGCGAGACAACAGGTACACCAGCG16480.12464829213687109No Hit
GTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGCTGCTTCTCCTTCTTGGC16300.12328684234411401No Hit
GTTGTGTATTTTTCTAAGCATGACAACATTGATGTGCCTTTTCAGTGTAA16220.12268175354733307No Hit
GGGTAGCTATGTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAA16200.12253048134813783No Hit
ATCTTAGCTTAACGAAACTAAGTAATTTTTGTTTATGATTATAGAACTGC16160.12222793694974737No Hit
ATCTAAAAATGTATGTGCTAACTATATCATCCAGTGTGCAGTGTTGTGTA16140.12207666475055215No Hit
GTTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGTGGGGGAAAGGC16080.12162284815296644No Hit
GTATATGAGTGCATTCTCGTAAGAATGAAGACAGTGAGGGGTTGGGGGAA16040.12132030375457599No Hit
CCCCAGAGGAGTGTTCTGGACTACAGCCTTGTCTTATGGTCAAATTGATA15940.12056394275859982No Hit
GTGGAGGTCCTAGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGC15870.12003449006141652No Hit
GATAAAAGGTACTCTGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA15860.1199588539618189No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT15810.1195806734638308No Hit
GTAAAAATAATAGTGATCAGGCAGAAAAGAAAAATGGAACATCTAAAAAT15710.11882431246785466No Hit
GGACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGG15500.11723595437630471No Hit
CTTCAAACACACTGCACTTAGAAACCAGACTTGAAATAATCGAAGCCCCA14930.1129246966992406No Hit
GGGCACCGGCGTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGCTGCTTCT14930.1129246966992406No Hit
TCCACACCCAGCCTATCAACGTCCTGGTCTCGAACGACCCTTCAGGGAGA14850.11231960790245969No Hit
GACAAGCGCCTGGTGGAGGAGAAAGTGAACCAACTGAAGGAGGAAGTTCG14690.11110943030889783No Hit
ATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTGAT14670.11095815810970261No Hit
GTCCTGTTCGGGATGGGAAGGGGTGGTACCGACTCGCTATGGTCACCAGG14630.11065561371131213No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCTGGGGAT14480.10952107221734789No Hit
CCCTATAACTACTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAA14240.10770580582700512No Hit
TGATTATAGAACTGCAGGATTCACAAGTAGTATTAGAGACATTCCACACT14210.10747889752821226No Hit
GTATATAGAGAGCTTCACCCAGGGCTCCATCGAGGCCCACAAGAGGGGCT14120.10679817263183372No Hit
GAATAAGAAAGTCACAAGATTGAGGAGTCTGTTCTAAAGTTCTTTGCTCC14070.10641999213384563No Hit
CCCTGAAGGGTCGTTCGAGACCAGGACGTTGATAGGCTGGGTGTGGAAGC13980.1057392672374671No Hit
GCTATGTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAACTTGC13930.10536108673947903No Hit
GTCTTATGGTCAAATTGATACCCTTAATAAGAAAGGAAAGGAAAGGAAAA13790.1043021813451124No Hit
CCCTAGGAGCGCTGTGGCTCCTACAATTAGCGCAGGCCCAGAGGGTTCAG13770.10415090914591715No Hit
GCAGGAAGGGCACCGGCGTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGC13700.10362145644873386No Hit
TCCTGGGGCTGTAGCCGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGA13540.102411278855172No Hit
GGTTAGCTGAACGCATTACTGCGCTTCCACACCCAGCCTATCAACGTCCT13440.10165491785919584No Hit
GTTTTGAGGGAGAGCCTGGCGATGACCTACTTTCACACGGGAATCCGCAC13400.10135237346080538No Hit
CTTCCGTACATAGCTACCCTGCGATGCCTCTGGCGAGACAACAGGTACAC13380.10120110126161015No Hit
CTCTGGGCCTGCGCTAATTGTAGGAGCCACAGCGCTCCTAGGGTGAGAGG13290.10052037636523159No Hit
GTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAACTTGCCTGAA13260.10029346806643875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTAA100.007091434144.99785145
TACGCCC151.230167E-4144.99785145
GAGCGAA1050.0117.4324951
ATAATAG15000.094.742673
TAATAGT15900.089.369744
AATAGTG15900.088.913775
TTAGATC353.4379413E-582.85591145
TACTGCC406.6644716E-572.4989247
ATACCTT1800.072.4989244
ACATAGT5100.068.242023
ATTGATC4100.067.194128
GGCATAC1950.066.952471
CATAGTG5200.066.922084
AGCGAAA12400.065.512631
ATAGTGA22250.063.538386
GCATAAC350.003565365262.1489832
TCTTTTA702.333527E-762.1489832
CCGTCAG350.00356696862.1419339
CAAGAGA350.00356696862.1419334
TAGGTTG954.5656634E-1061.0517235
Produced by FastQC (version 0.11.5)