FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_268_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_268_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1326875
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAATAGTGATCAGGCAGAAAAGAAAAATGGAACATCTAAAAATGTAT94350.7110692416391898No Hit
TTATAGAACTGCAGGATTCACAAGTAGTATTAGAGACATTCCACACTGCA67530.5089401789919925No Hit
GTCCTAGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGCGCAGGC55760.4202355157795572No Hit
ATCTTAGTGGGGGAAAGGCTGCTGCTCGTGCAGGTCTGGGTCCTGTCCAT40960.3086952425812529No Hit
GTATTAGAGACATTCCACACTGCACACTCCAGTCATATCTCTTAACAAAA40480.3050777202072539No Hit
ATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTG40420.304625529910504No Hit
GTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGAGAAAGTGAACCAAC39400.296938294865756No Hit
CTCCAGTCATATCTCTTAACAAAATGACATAAATTGACAATGTTCACTAA39290.29610927932171455No Hit
GGTCTAAAGAACGCACTTGAAAGTTGCAAAATTACTTGCCAATGTTTGGG36270.27334903438530384No Hit
GGTCTGGGTCCTGTCCATCAGGATTTTCACAGACTTGGTGTTCTTCATGA36010.2713895430993876No Hit
GTATATGGTATTGGGGGCAGGGAGAACCAGAAACCCTCAAAGAGAACCAA35510.267621290626472No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCTGGGGATAACAG33590.25315120113047573No Hit
GGGTATGGCTGTTCGCCATTTAAAGAGGTACGTGAGCTGGGTTTAAAACG32140.24222326895902024No Hit
GTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTGATGTGCCTTTTC30630.2308431464908149No Hit
GCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGAGAAAGTGAACC28310.21335845501648612No Hit
GTGGAGGAGAAAGTGAACCAACTGAAGGAGGAAGTTCGGCTGCAGTACGA28310.21335845501648612No Hit
CTACTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAAGAACTCTT27890.21019312293923692No Hit
CTCTAATACTACTTGTGAATCCTGCAGTTCTATAATCATAAACAAAAATT27560.20770607630711257No Hit
GTATTGGGGGCAGGGAGAACCAGAAACCCTCAAAGAGAACCAATTTGTAG26500.19971738106453132No Hit
TGACTGGAGTGTGCAGTGTGGAATGTCTCTAATACTACTTGTGAATCCTG26180.19730569948186527No Hit
TTCTAGCATTTTCCGCAGCTGCTTCTCCTTCTTGGCCACTTCGTTCAGGA25110.18924163918982573No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGA24860.1873575129533679No Hit
AAGCAGGACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCT24550.18502119642016013No Hit
ATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAGGA23910.18019783325482808No Hit
GTACGAGAAGCTGCACCAGCTGCTGGACGAGGACCTGCGGCAGACAGTGG23800.1793688177107866No Hit
GTTCTATAATCATAAACAAAAATTACTTAGTTTCGTTAAGCTAAGATTGT23370.17612812058407915No Hit
GGCTATGACTTGTCGCGTCGCTGACTCGATGGTCAACGCCGCCAATATGG23220.17499764484220443No Hit
ACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGGAG22660.17077720207253885No Hit
GTGTTAATACTTGGGCATTAATCTTCAGGCACCTCCAGTGAGGCAAATGA21200.15977390485162507No Hit
GTCCCAAGGGTATGGCTGTTCGCCATTTAAAGAGGTACGTGAGCTGGGTT21110.15909561940650022No Hit
GACTTGGTGTTCTTCATGAAGCTGACATCCTCCGTCTTATCAAAGAGCAG21050.15864342910975035No Hit
GTGTGGAATGTCTCTAATACTACTTGTGAATCCTGCAGTTCTATAATCAT20980.15811587376354216No Hit
GGACATAGTGATCCGGTGGTTCTGTATGGAAGGGCCATCGCTCAACGGAT20020.15088082901554403No Hit
CTATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACAT20000.15073009891662742No Hit
GCCTATCAACGTCCTGGTCTCGAACGACCCTTCAGGGAGATCAAGTCTCC19380.14605746585021195No Hit
TTCTTGGCCACTTCGTTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTT18780.14153556288271316No Hit
ATATGAGAGTTTCCTCCAGAGCCATCTTCCAAAGTGGCAGACTTGGCCAA18470.1391992463495054No Hit
GTTCTGGACTACAGCCTTGTCTTATGGTCAAATTGATACCCTTAATAAGA18410.13874705605275553No Hit
CTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAAGAACTCTTCCT18400.13867169100329724No Hit
AACTATATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAAC18290.13784267545925577No Hit
GGCAATTAGTACTGGTTAGCTGAACGCATTACTGCGCTTCCACACCCAGC18180.1370136599152143No Hit
ATTCCACACTGCACACTCCAGTCATATCTCTTAACAAAATGACATAAATT17710.13347150259067359No Hit
CTCCTAGGGTGAGAGGCTTAGCCATCCCTGACCCTGGCAGTGCACTGGTA17700.13339613754121527No Hit
GTTCTAAAGTTCTTTGCTCCTTGATGTCGAAGTTAACACAAACACAATCT17350.13075836081017428No Hit
GTGTGTATCAGGCACAAGGCAGACCCAGCCGGAAACATACCGGTTATAGG16940.12766839378238343No Hit
TAGCTACCCTGCGATGCCTCTGGCGAGACAACAGGTACACCAGCGGTACG16930.1275930287329251No Hit
GTTTGAAATTGATCCCTTATTTTACATGAAATAAAAACAATTTCTGTTGC16780.12646255299105041No Hit
TCTAAAAATGTATGTGCTAACTATATCATCCAGTGTGCAGTGTTGTGTAT16670.12563353744700895No Hit
GTACATAGCTACCCTGCGATGCCTCTGGCGAGACAACAGGTACACCAGCG16560.1248045219029675No Hit
AGCTAAGATTGTGTTTGTGTTAACTTCGACATCAAGGAGCAAAGAACTTT16550.12472915685350919No Hit
GTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGCTGCTTCTCCTTCTTGGC16330.1230711257654263No Hit
GTTGTGTATTTTTCTAAGCATGACAACATTGATGTGCCTTTTCAGTGTAA16270.1226189354686764No Hit
GGGTAGCTATGTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAA16250.12246820536975978No Hit
ATCTAAAAATGTATGTGCTAACTATATCATCCAGTGTGCAGTGTTGTGTA16210.12216674517192651No Hit
ATCTTAGCTTAACGAAACTAAGTAATTTTTGTTTATGATTATAGAACTGC16190.12201601507300988No Hit
GTATATGAGTGCATTCTCGTAAGAATGAAGACAGTGAGGGGTTGGGGGAA16120.1214884597268017No Hit
GTTCAGGAAGAGCTTGGAGTTCAGGTGGCCGATCTTAGTGGGGGAAAGGC16110.12141309467734339No Hit
CCCCAGAGGAGTGTTCTGGACTACAGCCTTGTCTTATGGTCAAATTGATA16040.12088553933113518No Hit
GATAAAAGGTACTCTGGGGATAACAGGCTGATACCGCCCAAGAGTTCATA15910.11990579368817711No Hit
GTGGAGGTCCTAGACAAGGCCCAGGCCAAGTTCTGCAGCGAGAACGCAGC15900.11983042863871879No Hit
GGGGATAACAGGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTT15850.11945360339142722No Hit
GTAAAAATAATAGTGATCAGGCAGAAAAGAAAAATGGAACATCTAAAAAT15760.1187753179463024No Hit
GGACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTGGTGG15530.11704192180876118No Hit
CTTCAAACACACTGCACTTAGAAACCAGACTTGAAATAATCGAAGCCCCA14960.11274611398963731No Hit
GGGCACCGGCGTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGCTGCTTCT14960.11274611398963731No Hit
TCCACACCCAGCCTATCAACGTCCTGGTCTCGAACGACCCTTCAGGGAGA14900.11229392369288742No Hit
GACAAGCGCCTGGTGGAGGAGAAAGTGAACCAACTGAAGGAGGAAGTTCG14740.1110880829015544No Hit
GTCCTGTTCGGGATGGGAAGGGGTGGTACCGACTCGCTATGGTCACCAGG14710.11086198775317946No Hit
ATCATCCAGTGTGCAGTGTTGTGTATTTTTCTAAGCATGACAACATTGAT14700.11078662270372114No Hit
GTTCTGTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCTGGGGAT14520.10943005181347151No Hit
CCCTATAACTACTATACAACTGAAGAATTGAAGGGGGGGGACACCACCAA14280.10762129062647198No Hit
TGATTATAGAACTGCAGGATTCACAAGTAGTATTAGAGACATTCCACACT14230.10724446537918039No Hit
GTATATAGAGAGCTTCACCCAGGGCTCCATCGAGGCCCACAAGAGGGGCT14160.10671691003297222No Hit
GAATAAGAAAGTCACAAGATTGAGGAGTCTGTTCTAAAGTTCTTTGCTCC14140.10656617993405558No Hit
CCCTGAAGGGTCGTTCGAGACCAGGACGTTGATAGGCTGGGTGTGGAAGC14020.10566179934055582No Hit
GCTATGTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAACTTGC13970.10528497409326425No Hit
GTCTTATGGTCAAATTGATACCCTTAATAAGAAAGGAAAGGAAAGGAAAA13820.10415449835138954No Hit
CCCTAGGAGCGCTGTGGCTCCTACAATTAGCGCAGGCCCAGAGGGTTCAG13800.10400376825247291No Hit
GCAGGAAGGGCACCGGCGTGCTGAAGGGGCCTTCTAGCATTTTCCGCAGC13720.1034008478568064No Hit
TCCTGGGGCTGTAGCCGGTCCCAAGGGTATGGCTGTTCGCCATTTAAAGA13560.10219500706547338No Hit
GGTTAGCTGAACGCATTACTGCGCTTCCACACCCAGCCTATCAACGTCCT13460.10144135657089025No Hit
GTTTTGAGGGAGAGCCTGGCGATGACCTACTTTCACACGGGAATCCGCAC13450.10136599152143194No Hit
CTTCCGTACATAGCTACCCTGCGATGCCTCTGGCGAGACAACAGGTACAC13410.10106453132359867No Hit
CTCTGGGCCTGCGCTAATTGTAGGAGCCACAGCGCTCCTAGGGTGAGAGG13330.10046161092793218No Hit
GTACGGAAGAGATAACCGCTGAAAGCATCTAAGCGGGAAACTTGCCTGAA13280.1000847856806406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA700.0124.311591
ATAATAG15050.084.310353
TAATAGT15600.081.33484
AATAGTG15900.079.8001865
ATACCTT1400.072.5041664
ACATAGT5550.069.2408143
CATAGTG5650.068.012764
GGCATAC1500.067.6807561
AGCGAAA10850.064.829161
AGTCTAA606.8029785E-660.4224132
AGGACAT8250.058.0033345
GGGCGAC1251.8189894E-1257.9989627
GGCGACC1251.8189894E-1257.9989628
GCGAAAG12450.056.4913182
AGGTCTT1800.056.3900036
GAGTCTA651.0924923E-555.780841
ATAGTGA23100.055.239196
CGAAAGC12900.054.520693
AGGGCGA1353.6379788E-1253.7047656
TACTAGC701.6953338E-551.790642
Produced by FastQC (version 0.11.5)