FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_231_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_231_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966134
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT69270.35231576281169036No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT52750.2682930054614792No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT41220.20965000350942511No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT39840.20263115331915324No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA36250.18437197057779378No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT29410.14958288702601144No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT28580.14536140466519576No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT27220.13844427694144956No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT26410.13432451704715956No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA26140.13295126374906288No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC25590.13015389591960672No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC25570.130052173453081No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT24860.1264410258914194No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA24660.12542380122616262No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA24410.12415227039459162No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGTTACA24010.12211782106407802No Hit
TTATAGATATTTCTTCACAAGAGCTGAATTTCCCATGGAGCTCTGCTTTA23580.11993078803377592No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC23550.1197782043339874No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATCCCTTCCCCATTCTTTTGA23280.11840495103589074No Hit
GTTCATAGCTGAGACCATCTGCATTTCTCGTCTCACTCCAGGCACTGAAG23190.1179471999365252No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAAAAAGC23180.11789633870326235No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT23050.11723514267084542No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT22610.11499724840728048No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT22330.11357313387592098No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC21810.11092834974625332No Hit
GCTATGAGCCCTGTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGA21530.10950423521489379No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC21360.10863959424942551No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC21250.10808012068353429No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA21230.10797839821700861No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC20600.1047741405214497No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA20150.10248538502462191No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT19900.10121385419305093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGCA100.0070904433145.00641145
CCATCCG100.0070920605144.995358
AGCACGC8750.0128.466931
CACGCAC8950.0125.576983
GCACGCA8950.0125.576982
CGGTGCA750.0116.016942
GTAACAA12450.0112.988384
GAAACAC6100.0101.039344
AAACACG6500.094.821535
CGCACTT12400.091.807755
ACGCACT12400.091.2229844
ACGTGCA2600.089.24382
AACACGT1700.085.304396
CAACGCA3400.085.291389
TCTACTA554.3921318E-879.102455
CACGTGC4050.078.7889561
ACACGTG1850.078.3758547
CACCGTT755.638867E-1177.344625
TAGAAAC9350.074.449362
TCACCGT809.822543E-1172.510584
Produced by FastQC (version 0.11.5)