FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_2018_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_2018_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077846
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA93610.45051461946650523No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT76630.36879537752075947No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT68940.3317858975111726No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT68680.33053460169810467No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC64410.30998447430656556No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT60440.2908781497762587No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG59220.2850066848072475No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA57600.2772101493565933No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA57450.2764882479259772No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC56840.27355251544147163No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT53530.2576225572058757No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT53320.2566118952030131No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG48770.2347142184743239No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA42700.20550127391539122No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT41910.2016992597141463No Hit
GCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAGAAGACGT41660.20049609066311944No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC41540.1999185695186265No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA40540.19510589331451897No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT39430.18976382272795964No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA38520.1853842873822218No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38390.1847586394756878No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT38050.18312232956629124No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT37540.18066786470219642No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA34500.16603732904170954No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC33580.1616096669339306No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC32170.154823793486139No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG32090.1544387793898104No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT31920.15362062443511215No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT31810.15309123005266032No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC31470.15145492014326375No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG31280.15054051166448332No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT30470.14664224393915623No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA30220.14543907488812935No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC29900.14389901850281492No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGACACTGCA29570.14231083535545944No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA28140.1354287083835857No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT27650.13307049704357302No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA27480.13225234208887474No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT27470.13220421532683366No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG27260.1311935533239711No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC26920.12955724341457453No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT26480.1274396658847672No Hit
GCACAGAGCGTTCCTAGTTTCACTTGCATTGAATAATTTCCAGGATTCAG25860.12445580663822055No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAT25510.12277136996678292No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCGAAATTGAAAAGGCTGAA25430.12238635587045431No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA24880.11973938395819517No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG24840.11954687691003087No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC24550.11815120081083968No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT23120.11126907383896592No Hit
GTATAGCACCTGTCTTGTTCTCCAACAAAATAGCCAGATTAGGGAAAGGA22630.10891086249895324No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG22320.1074189328756799No Hit
GTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTCTT22260.10713017230343347No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA22260.10713017230343347No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC21990.10583074972832442No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAACTATGAGTTCCAGA21950.10563824268016013No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT21880.10530135534587261No Hit
GTCCTGATCTGTTCAGCATACCATTAGAAAGATATAATGAAGAAACAAGG21780.10482008772546185No Hit
AAATTATTCAATGCAAGTGAAACTAGGAACGCTCTGTGCTTTGTGCGAAA21770.10477196096342078No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA21550.10371317219851713No Hit
CCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCCCC21320.10260625667157239No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT21100.10154746790666874No Hit
TCTGCATACTGTCCAATCGAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA21020.10116245381034013No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG20960.10087369323809368No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT20780.10000741152135433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCC100.0070917173144.99788145
GAGCAGA1600.0131.448621
AGCACGC4650.0112.294261
AACACGT1100.0112.0573046
AAACACG3500.0111.8797455
GCACGCA4700.0111.091612
CACGCAC4800.0108.777213
GAAACAC3600.0108.7719654
ATAAGCC203.866874E-4108.74841145
ACACGTG1250.098.598567
AGCGTTT301.5999829E-596.665268
ACGTGCA607.2759576E-1296.665269
CACGTGC1300.094.806318
AACACGA2850.094.132766
ACACGAG2950.090.930887
ACAACGC4250.088.704588
ACGAGCC1650.087.877519
ACGTGCC750.086.9987269
CAACGCA4500.083.776559
AACAACG4650.081.074097
Produced by FastQC (version 0.11.5)