FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_2017_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_2017_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585115
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT41490.26174757036555707No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA29350.1851600672506411No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC28220.17803124694422803No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT26950.1700192099626841No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG26160.16503534443873158No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT25740.16238569441333908No Hit
CTTCTGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC25390.16017765272551202No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT24980.15759108960548604No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCGGG24250.15298574551373245No Hit
ATGCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA22830.14402740495169122No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT22740.1434596228033928No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT22210.14011601681896896No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA22060.13916971323847166No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC21250.13405967390378615No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT21180.13361806556622075No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT20770.13103150244619474No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG20450.12901272147446716No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA20240.12768789646177092No Hit
GTCTATAGCTGTCAGACATGATTTTAAAGGTTCAGTATCTATCACGGTCT19520.1231456392753838No Hit
CATATGGACCAATAGGAGATACTGAAGGTTTCGAAAAAGTCTACGAAAGC19370.1221993356948865No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC19360.12213624878952001No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG19300.12175772735732109No Hit
CTCCTAATTAGCCCCCATAGATTCGGAGAAACCAAAGGAAACTCAGCTCC19170.12093759758755673No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC19140.12074833687145728No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT18780.11847720827826372No Hit
GCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCATC18750.11828794756216425No Hit
GTCATAATGGGAGCCAATGACGTAAGTGAATTAGAATCACAAGCACAACT18630.11753090469776639No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG18620.11746781779239993No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACATAC18300.11544903682067231No Hit
GTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCACGGGCAGGA18090.11412421180797608No Hit
TCCTAATGCAGCTCTTATTATGTCACAGGCGACATCACCTCCGTCTATAG17760.112042343930882No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG17710.11172690940404954No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG17570.11084369272891872No Hit
GTACTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCCCC17320.10926652009475653No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA17110.1079416950820603No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT17070.10768934746059435No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAGTTACAGAAAACC16800.10598600101569917No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA16630.10491352362446889No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT16590.10466117600300294No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTT16440.10371487242250563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGT700.0113.940576
GAAACAC2400.0111.793194
GAGCCCC203.8663147E-4108.75116145
CGAGACC203.8663147E-4108.75116145
AAACACG2500.0107.321455
AACACGA1800.0104.733256
ACGTGCC352.7628448E-7103.569269
ACACGAG1900.099.208457
GAGCAGA1050.096.71041
CACGAGC2100.089.760048
AGCACGC502.262459E-887.039371
CCATGCC259.388547E-486.9981849
TAGAAAC3600.084.602922
CACGTGC950.083.945628
ACGAGCC900.080.553889
ACAACGC1000.079.748348
ACACGTG1000.079.748347
CAACGCA1050.075.95089
AAGCGGA1400.072.5167852
ACGTGCA608.0359314E-872.498499
Produced by FastQC (version 0.11.5)