FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_2017_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_2017_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591460
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT41630.26158370301484174No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA29430.1849245347039825No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC28320.17794980709537153No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT27000.16965553642567202No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTAGTTTTAGACATCGTCCGAG26210.1646915411006246No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT25910.16280647958478378No Hit
CTTCTGTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATC25500.160230228846468No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT25110.15777964887587498No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGGCAAATATATCACCCGGG24390.15325550123785708No Hit
ATGCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCA22940.14414437057795987No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT22840.14351601673934625No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT22290.14006007062697146No Hit
TACCATTCCTATGCAAAAAACATCCTAAGGACACAAGAAAGTGCCTGCAA22180.1393688814044965No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC21310.13390220300855818No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT21280.1337136968569741No Hit
GTATTGTTCCGTTCCCGATTAATATTTCTTCATCATTTTTGAATCCTCTT20800.13069759843162881No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG20540.12906387845123346No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA20320.12768150000628353No Hit
GTCTATAGCTGTCAGACATGATTTTAAAGGTTCAGTATCTATCACGGTCT19580.1230316816005429No Hit
AGCATATACTGACACATACCATTCCTATGCAAAAAACATCCTAAGGACAC19450.12221482161034522No Hit
CATATGGACCAATAGGAGATACTGAAGGTTTCGAAAAAGTCTACGAAAGC19420.12202631545876115No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG19370.12171213853945434No Hit
CTCCTAATTAGCCCCCATAGATTCGGAGAAACCAAAGGAAACTCAGCTCC19240.12089527854925665No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC19190.12058110162994987No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT18830.11831902781094089No Hit
GCATTACTGTCTGGGCCATCAACTCCGATGTAAGTCCATTCTCTACCATC18820.11825619242707955No Hit
GTCATAATGGGAGCCAATGACGTAAGTGAATTAGAATCACAAGCACAACT18700.11750216782074321No Hit
GGAATGGTATGTGTCAGTATATGCTTCTCCATATTTTATTTTGAGCAATG18690.11743933243688186No Hit
GTAATGCATTGCTCAAAATAAAATATGGAGAAGCATATACTGACACATAC18390.11555427092104105No Hit
GTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCACGGGCAGGA18150.11404622170836842No Hit
TCCTAATGCAGCTCTTATTATGTCACAGGCGACATCACCTCCGTCTATAG17810.11190981865708217No Hit
GCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTTGATAAAG17780.1117213125054981No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG17650.11090445251530041No Hit
GTACTGAAGGGAGGAGGAGAAAATTTCATCGAAGTAAGGAAAGGGTCCCC17360.10908222638332098No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT17180.10795118947381649No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA17180.10795118947381649No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAGTTACAGAAAACC16900.10619179872569842No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA16700.10493509104847122No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT16640.10455807874530304No Hit
GGATAAAGCACTATATCTTTTCCTTTTAACTACTTTAACGGGTTTTCCTT16500.103678383371244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACACG100.007088919145.015985
TCTAAAT100.007088919145.015982
GTACCCC100.0070902514145.00685145
TTATACC100.0070902514145.00685145
AACACGT650.0111.547236
GAAACAC2300.0104.0331954
AAACACG2350.0101.819725
AACACGA1900.095.4022456
ACGAGCC700.093.212839
TAGAAAC3350.090.9055252
GAGCAGA800.090.652071
AGCACGC406.1082756E-790.652071
ACACGAG1950.089.229387
ACAACGC950.083.946068
CAACGCA950.083.946069
CACGAGC2150.080.928978
ACACGTG900.080.5543067
TAGTAGA608.0286554E-872.507983
CGAGACC300.001935590472.503426145
AACAACG1100.072.498877
Produced by FastQC (version 0.11.5)