FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_2016_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_2016_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1559555
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA155000.9938732523059463No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT84980.5448990256836085No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA76760.4921916828838996No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT67010.4296738492711062No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT62970.4037690238561641No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG62070.39799814690729085No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT61830.3964592463875914No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT53680.34420074957279484No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT52820.3386863560438715No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA50850.32605454761133784No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC50820.32586218504637543No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC49190.3154104856834161No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC49160.31521812311845365No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG47500.30457406119053193No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC47160.3023939521209576No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT46970.3011756558761955No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT46600.2988031842416587No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT44910.2879667597487745No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA44590.2859148923891751No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT42550.2728342379717291No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG42150.2702694037722299No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC36570.23448996668921585No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC36490.233976999849316No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA36260.23250222018460392No Hit
GCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAGAAGACGT35290.22628249725081834No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT33010.2116629423136728No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT32530.20858514127427377No Hit
CAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATAAGA32290.20704624075457423No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT32220.20659739476966188No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA32140.20608442792976203No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC30790.19742811250645217No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT30710.19691514566655233No Hit
GTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTC30510.1956327285668027No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA30260.1940297071921157No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA30190.19358086120720333No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC29020.18607872117366814No Hit
TGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCT28960.18569399604374323No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT28890.18524515005883088No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG28230.1810131736296572No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT27340.17530641753577142No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT26420.16940729887692324No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA26290.16857372776208598No Hit
GGACAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATA26190.16793251921221117No Hit
CTCAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC25890.16600889356258677No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA25600.16414938876794982No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT25280.16209752140835046No Hit
GCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTC24910.1597250497738137No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG24640.1579937866891517No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT24160.15491598564975265No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG24110.15459538137481524No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATT23240.14901686699090444No Hit
TCTTTAGCCTGAATTTCTTTTTGGTAACTGTGTCTCAAAATTGATACCTC23150.14843977929601715No Hit
TTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTCTCCATTTCAT23000.14747796647120492No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT22700.14555434082158053No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT22610.14497725312669318No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA22500.1442719237218309No Hit
ATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTCTCCATTTCA22470.14407956115686849No Hit
GCACAGAGCGTTCCTAGTTTCACTTGCATTGAATAATTTCCAGGATTCAG22430.14382307773691855No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT22210.14241241892719397No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT22070.14151472695736925No Hit
AATAAAAAGAGGAGTAAACATGAAAATAAGAATAAAGGGGCCAAATAAAG21770.13959110130774482No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT21670.13894989275787004No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC21170.135743850008496No Hit
AGTCAGAAGAATGGGGAAGGAATTGCAAAGGATGTAATGGAAGTGCTAAA20940.13426907034378396No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT20880.13388434521385909No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG20720.1328584115340594No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA20650.13240956554914704No Hit
GAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTG20290.13010121476959774No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA20220.1296523687846854No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC20220.1296523687846854No Hit
ACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTTCAT20190.12946000621972292No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA19860.1273440180051361No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG19520.12516390893556176No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG18770.12035484481150072No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATT18620.1193930319866885No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT18580.11913654856673858No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG18480.11849534001686379No Hit
AAATTATTCAATGCAAGTGAAACTAGGAACGCTCTGTGCTTTGTGCGAAA18480.11849534001686379No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC18180.11657171436723938No Hit
CTTGTGAAGAAATACCTATAATGTTTGAACCATTCCAGATTCTTTCAATT18170.11650759351225189No Hit
TTTTTATTATCTCTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTC17910.1148404512825774No Hit
GTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGT17660.1132374299078904No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG17330.11112144169330353No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC17330.11112144169330353No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC17230.11048023314342874No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT17000.10900545347871668No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC16880.10823600321886691No Hit
CCTTTTCCCATCCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT16740.1073383112490422No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA16620.10656886098919242No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT16600.10644061927921747No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG16370.10496583961450542No Hit
GGCTTGGACAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGA16200.10387578507971824No Hit
AATAAAGAGACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCA16170.1036834225147558No Hit
TACCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATT16150.10355518080478086No Hit
GCTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA16110.10329869738483094No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA16040.10284985139991858No Hit
ACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGGCT15950.10227276370503124No Hit
CTGTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTAC15930.10214452199505629No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA15860.10169567601014391No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT15730.10086210489530667No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA15700.10066974233034424No Hit
CATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATAAGAATAAAGGG15690.10060562147535676No Hit
TGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTGTGAAGAAATACCTA15630.10022089634543188No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCC151.2291137E-4145.030272
TTACAGG100.0070868162145.030273
CAGGTAT100.0070874956145.025626
GAGCAGA800.0126.925911
AGCACGC7900.0112.006951
GCACGCA8000.0110.585582
CACGCAC7950.0110.368943
CGGTGCA553.6379788E-12105.4765552
GGTGCAT553.6379788E-12105.4765553
GAAACAC2450.0103.5930564
AAACACG2500.0101.5211945
AACACGT850.093.840116
ACAACGC8550.093.2733848
CAACGCA8900.089.605349
ACACGTG900.088.6097267
ACGAGCC608.2945917E-1084.582019
AACACGA2100.082.871796
AACAACG10300.077.4259647
ACACGAG2250.077.3321157
GCGGTGC1150.075.6825261
Produced by FastQC (version 0.11.5)