FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_2016_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_2016_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1565942
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA155540.9932679498985275No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT85290.5446561877770696No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA77040.4919722441827348No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT67220.42926238647408393No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT63220.40371865624652764No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG62310.3979074576197586No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT62150.3968857084106563No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT53900.3442017648163214No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT53060.3388375814685346No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA51050.32600185702918755No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC51020.32581027905248083No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCC49360.315209631008045No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC49330.31501805303133834No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG47680.3044812643124713No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC47420.30282092184768017No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT47190.3013521573595957No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT46760.2986062063601334No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT45080.2878778396645597No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA44810.2861536378741997No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGCGTTCCTAGTT42720.2728070388303015No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG42300.2701249471564081No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC36760.2347468807912426No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC36570.23353355360543368No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA36380.23232022641962471No Hit
GCTATGAACACAGCAAAAACAATGAATGGGATGGGAAAAGGAGAAGACGT35410.22612587183944233No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT33160.21175752358644193No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT32670.20862841663356627No Hit
CAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATAAGA32390.2068403555176373No Hit
CAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCT32310.2063294809130862No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA32270.20607404361081064No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAACTAGGAAC30970.1977723312868548No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT30800.19668672275218366No Hit
GTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTC30630.1956011142175125No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA30370.19394077175272137No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTGAAA30300.19349375647373912No Hit
TGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCT29070.18563905942876557No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC29070.18563905942876557No Hit
CTCAATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTT29010.18525590347535223No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG28420.18148820326678766No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT27410.17503841138432968No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT26480.16909949410642283No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA26370.16839704152516505No Hit
GGACAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATA26330.16814160422288948No Hit
CTCAATACTTCCATGTTGTCAGAGAGTACTTCCTTCATTGCTTCTTTAGC25950.16571494985127164No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA25770.1645654819910316No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT25380.16207496829384485No Hit
GCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTC25040.15990375122450257No Hit
GGATGTAATGGAAGTGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTG24740.15798797145743584No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG24240.1547950051789913No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT24230.1547311458534224No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATT23340.1490476658777911No Hit
TCTTTAGCCTGAATTTCTTTTTGGTAACTGTGTCTCAAAATTGATACCTC23230.14834521329653333No Hit
TTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTCTCCATTTCAT23110.14757890138970664No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCAGCAGAAAGCCCCT22770.14540768432036436No Hit
CCTTTATTCTTATTTTCATGTTTACTCCTCTTTTTATTTGATTTAAATGT22650.14464137241353767No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA22600.14432207578569323No Hit
GCACAGAGCGTTCCTAGTTTCACTTGCATTGAATAATTTCCAGGATTCAG22540.14393891983227985No Hit
ATTCCAGATTCTTTCAATTTGTTCTTTTATTTTATCAGCTCTCCATTTCA22540.14393891983227985No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT22280.14227857736748872No Hit
CAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATT22190.1417038434373687No Hit
AATAAAAAGAGGAGTAAACATGAAAATAAGAATAAAGGGGCCAAATAAAG21850.13953262636802638No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT21740.1388301737867686No Hit
GAATAATTTCCAGGATTCAGGTACATGACCATGAGACAATATAGTAGCGC21290.13595650413616853No Hit
AGTCAGAAGAATGGGGAAGGAATTGCAAAGGATGTAATGGAAGTGCTAAA21040.13436002099694624No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT20980.1339768650435329No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA20830.13301897515999955No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG20810.13289125650886177No Hit
GAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAACTG20360.13001758685826167No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC20320.1297621495559861No Hit
ACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTTCAT20310.12969829023041723No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA20260.12937899360257277No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA19910.12714391720766158No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG19620.12529199676616376No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG18870.12050254734849696No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGATTCATTGAACATT18710.1194807981393947No Hit
ATGCTTAACTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTT18630.11896992353484356No Hit
AAATTATTCAATGCAAGTGAAACTAGGAACGCTCTGTGCTTTGTGCGAAA18560.11852290825586133No Hit
TTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCTGCTTTCATTG18550.11845904893029245No Hit
CTTGTGAAGAAATACCTATAATGTTTGAACCATTCCAGATTCTTTCAATT18280.11673484713993237No Hit
TTCATGGCCTTCTGCTATTTCAAATGCTTCATGAAAGCTCACACATTTTC18250.11654326916322572No Hit
TTTTTATTATCTCTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTC17970.11475520804729678No Hit
GTCAGAGAGTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGT17720.1131587249080745No Hit
CTTCAGCAGAAAGCCCCTCAATTACTATGTGGTCACTCAATACTTCCATG17420.11124294514100778No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC17390.1110513671643011No Hit
CAATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTC17310.11054049255974999No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT17110.10926330604837216No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC16950.10824155683926989No Hit
CCTTTTCCCATCCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT16810.10734752628130545No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT16700.10664507370004764No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCAGCACTAGGCATCAAAA16660.10638963639777207No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG16480.10524016853753204No Hit
GGCTTGGACAATAGGACATTTAAATCAAATAAAAAGAGGAGTAAACATGA16220.10357982607274087No Hit
TACCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATT16220.10357982607274087No Hit
AATAAAGAGACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCA16220.10357982607274087No Hit
GCTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA16210.10351596674717199No Hit
TAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCATGAA16120.10294123281705198No Hit
ACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGGCT16020.10230263956136307No Hit
CTGTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTAC16010.10223878023579419No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA15940.10179176495681194No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT15780.10077001574770969No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA15740.10051457844543411No Hit
CATTTAAATCAAATAAAAAGAGGAGTAAACATGAAAATAAGAATAAAGGG15740.10051457844543411No Hit
TGCTAAAGCAGAGCTCTATGGGAAATTCAGCTCTTGTGAAGAAATACCTA15700.10025914114315856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCC100.0070914906144.9983145
GAGCAGA700.0134.69721
AGCACGC7800.0120.88211
CACGCAC8100.0117.285423
GCACGCA8050.0117.113032
AAACACG2900.0115.028045
GAAACAC2950.0113.078414
CGGTGCA352.7579517E-7103.599992
AACACGT1300.0100.402686
ACGTGCC301.5998365E-596.6655359
ACACGTG1400.093.21327
AACACGA2300.088.276766
CGGTGCC259.3774684E-487.0239942
ACACGAG2350.086.381977
ACAACGC8150.086.287348
CACGTGC1600.081.561558
CAACGCA8700.080.832399
CGGTGCG554.3886757E-879.11025
CACGAGC2650.076.6028758
TAGAAAC9750.075.867073
Produced by FastQC (version 0.11.5)