FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_2012_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_2012_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773527
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTC113460.6397421634968061No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT102260.5765911655136912No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAG99270.5597321044449844No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT85460.4818646685390186No Hit
GGTTTATGCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA84400.4758878776584738No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA77620.4376589699508381No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT63620.35872022247194435No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAAAGTC63130.35595736631018304No Hit
CCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTG60560.34146646766584327No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC55480.3128229792949304No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT54090.3049854893666688No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT52990.29878315920761284No Hit
GCATAAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAATGTAA51030.2877317345605677No Hit
CCTTATTTCCTCAAATTTCCGTCCCAATTGTTCCCGCCACTTCTCATTTC48090.27115459759000005No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT47400.26726404503568313No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA47160.2659108093646164No Hit
ACATAATGGAATCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTTCTT46810.263937340677644No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC46640.26297879874397173No Hit
GTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT43980.24798043672298195No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT40540.2285840587710252No Hit
CTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAAT40070.2259339722485195No Hit
GATAAGAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATT39840.2246371213970805No Hit
TCTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCT39220.22114126258015807No Hit
GCTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA38100.21482616278184657No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA38100.21482616278184657No Hit
GTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCTTATTAGCAAC37220.2098642986546018No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA36940.20828552370502396No Hit
AGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAG35740.2015193453496902No Hit
GTGCCGTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCCGTCCCA34790.19616278748505098No Hit
ACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCC34630.19526063037100647No Hit
GTATAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT34430.19413293397845086No Hit
ATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA34400.1939637795195675No Hit
ATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTT33890.19108815371855067No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCA33820.1906934599811562No Hit
GTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG32830.18511136283800583No Hit
GGATAGAGCAGTTAAACTATACAAGAAGCTCAAAAGAGAAATAACGTTCC32620.18392728162582245No Hit
GTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTTGCTTCCAGT32030.18060057726778334No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTT31920.17998034425187776No Hit
CTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGAAAGAGGAATCCAGC31210.1759770220583053No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT31040.175018480124633No Hit
GAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGA30830.1738343989124496No Hit
CTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT30810.17372162927319404No Hit
GGCATAAGCATAAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA30800.17366524445356626No Hit
GCTTATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACC30590.17248116324138285No Hit
GTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGACCTTGAT30570.17236839360212727No Hit
CTCTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAAT30320.17095877311143276No Hit
CAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTACGCCGAGATCAAA30190.17022577045627157No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTC30030.1693236133422271No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGCTTCTCCAAGCG29520.16644798754121026No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT29430.16594052416456023No Hit
CTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCG29400.16577136970567688No Hit
GCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG29320.16532029114865462No Hit
GAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACTCTTGTTGGG28970.16334682246168228No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC28940.16317766800279893No Hit
GTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGATCCAGC28840.16261381980652112No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTTATGCTTATG28690.1617680475121044No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC28240.15923073062885426No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG28150.15872326725220423No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA27980.15776472531853195No Hit
GTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCACG27730.15635510482783743No Hit
GTTGAAGATTATACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGA27620.15573487181193182No Hit
ACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAACCGATTAGAGACC27290.15387417276421506No Hit
GTCATGGATAAGAACATAGTACTGGAAGCAAACTTCAGTATAATCTTCAA27210.15342309420719277No Hit
CTTTTCAAACCGTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACG26640.15020915948840927No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATTAACCACCTTA26610.1500400050295259No Hit
CATCTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATG26210.14778461224441464No Hit
GTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCAC26140.14738991850702018No Hit
GCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGCCTGTTCACTCGAT25770.1453036801807923No Hit
CTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCTCTTTTCAAACCGTA25740.14513452572190894No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGAC25610.14440152306674778No Hit
CTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTC25350.14293551775642546No Hit
TCCTAGCTCCAGTGCTGGTCTGAGAGATGACCTTCTTGAAAATTTGCAGG25260.14242805437977543No Hit
CTCCCATTCTCATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGA25010.14101843388908092No Hit
AAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGCTTTC25010.14101843388908092No Hit
GTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGGATTGGGTGAT24930.14056735533205866No Hit
TCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTT24930.14056735533205866No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGAATCCAACACCATGTCAAGC24710.13932688930024748No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCG24700.1392705044806197No Hit
ATACTGAAGTTTGCTTCCAGTACTATGTTCTTATCCATGACCGCCTGGTC24690.1392141196609919No Hit
CTTCAGTATAATCTTCAACCGATTAGAGACCTTGATACTACTAAGGGCTT24540.1383683473665752No Hit
CCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTT24380.1374661902525307No Hit
ATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGT24080.13577464566369726No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCACTCAAGTC24040.1355491063851861No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAAATGGCT24030.13549272156555836No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG24000.135323567106675No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT23590.13301178950193596No Hit
CATCACAGCTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACC23540.13272986540379705No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC23320.13148939937198587No Hit
GTATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCCGTCCCAATTGT23020.12979785478315245No Hit
GTTCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGC22710.12804992537469123No Hit
TCCATAGCCTTTGCCGTAGTGCTAGCCAGCACCATTCTGTTTTCATGCCT22530.12703499862139117No Hit
CACTTAGAATGACAATTGCATCTGTACCTACTTCGCGTTACATTTCTGAC22470.12669668970362447No Hit
CCACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACTCAGGC22370.12613284150734666No Hit
CTATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAAT22210.12523068439330218No Hit
GCATGTACCATCTGCCTAGTCTTATTAGCAACCTCCATGGCCTCTGCTGC22030.1242157576400021No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAGTGAAC21750.12263698269042422No Hit
CAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACAATGG21680.12224228895302974No Hit
CTTATCTCTTGCTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGT21550.1215092862978686No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATT21530.12139651665861304No Hit
CTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCAT21340.12032520508568519No Hit
CCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAAAACAGCCACT21280.11998689616791851No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATAAGCA21040.11863366049685176No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC21010.11846450603796842No Hit
GTAGTATCAAGGTCTCTAATCGGTTGAAGATTATACTGAAGTTTGCTTCC20820.11739319446504057No Hit
GTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAA20300.11446118384439595No Hit
TCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATGGGCCTCATATA20220.1140101052873737No Hit
GCACTGGAGCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTG20080.11322071781258475No Hit
CAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTG20050.11305156335370142No Hit
CTATACAAGAAGCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGT19890.1121494062396569No Hit
CATCAAAACAGCCACTCTTGTTGGGAAACAAATTGTGGAATGGATTTTGA19800.11164194286300687No Hit
TTCCAGTACTATGTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAA19710.11113447948635684No Hit
GGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGG19670.11090894020784572No Hit
GGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAG19590.11045786165082348No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG19540.11017593755268457No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA19530.11011955273305678No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTTCCATTTAGGGCATTTTGGAT19490.10989401345454566No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC19440.10961208935640675No Hit
GATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGGTCATGGATAAGA19310.1088790867012456No Hit
GATCAAAAGTCCTTAAAAGGAAGAGGCAACACCCTTGGCCTCGACATCAA19290.10876631706199004No Hit
CCTCAGTGAAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTGAAGATT19120.10780777512831775No Hit
GTTGATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCCT18950.10684923319464548No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTGGAA18940.1067928483750177No Hit
GTTCACAAGTGGCACAAACTAGACCAAAAGCAGCTTCTGTGGTCACTGTT18930.10673646355538992No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG18930.10673646355538992No Hit
GTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGGTGGCTGGATCGAG18900.10656730909650657No Hit
ATACAGAGATTCGCTTGGAGAAGCTGTGATGAGAATGGGAGACCTTCACT18890.10651092427687878No Hit
TCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGCTCTACTTCAA18870.10639815463762323No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT18740.10566515198246207No Hit
GGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGA18730.10560876716283428No Hit
TCCCAATTGTTCCCGCCACTTCTCATTTCTGCTCTGGAGGTAGTGAAGGT18620.10498853414692869No Hit
GACTCGAACCGTGTTACCATTCCACTCAAGTCCTCCGATGAGGACCCCAA18570.10470661004878978No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGTCAAGTGGTCAGCCTGATG18540.10453745558990643No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAAATCCATTCCACAATTTGT18300.10318421991883969No Hit
GTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGTATTTCTTCAATT18290.10312783509921192No Hit
CGATTGGACCAGGCGGTCATGGATAAGAACATAGTACTGGAAGCAAACTT18220.10273314136181745No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG18150.10233844762442296No Hit
CTGTACCTACTTCGCGTTACATTTCTGACATGACCCTCGAGGAAATGTCA18140.1022820628047952No Hit
CCACAATATCAGGTGCAAGATCCCAATGATGTTTGCTGCAATGACGAGAG18090.10200013870665628No Hit
GTTCACAGCAGTGGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA18040.10171821460851739No Hit
GTATTTAAAGCGACGATAAATGCATTTGAAAAAAAGACGATCAGTAATCC18020.10160544496926181No Hit
CTTTCAGGTAGACTGTTTTCTTTGGCATATTCGCAAGCGATTTGCAGACA17970.1013235208711229No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG17860.10070328785521732No Hit
GTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAGCTGTGATGA17750.10008305483931172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA800.0117.8406141
AGTAGAA6500.097.0616151
TCGCTCC259.386417E-487.003585
AGGAGCG259.38852E-486.998677
GCGAAAG5850.081.798252
GGTCAAA554.3892214E-879.109781
GTAGAAA14850.079.109781
CGAAAGC6100.078.4458543
CAGGTAC651.5643309E-978.080143
TTAACAG1700.076.76787145
TTCTACT1450.075.003094
GTTTAAA809.822543E-1172.5172961
TTCGCTC300.001935673672.502994
ATTGGGG1000.072.50298145
GAGCGCT300.001936106772.498899
TAGAAAC17150.068.4867862
AGAAACA17300.067.8929753
GTACTGA754.8567017E-967.667556
CTTTCGC350.003566376262.145422
TTTCTAC1750.062.1454163
Produced by FastQC (version 0.11.5)