FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_1230_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_1230_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433034
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAG209351.4608864828050137No Hit
GTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAA189911.3252302457583No Hit
GCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTA166671.1630568430337311No Hit
GTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAG135780.9475001988787426No Hit
CTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTG130980.9140048317067146No Hit
GTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCAC128180.8944658675230316No Hit
GGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTG105490.7361304756202575No Hit
GTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAAT88850.6200132027572269No Hit
AGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAAC87710.6120580530538703No Hit
CACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG86350.6025676990217956No Hit
GTAGAAACACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAG82430.5752131491646395No Hit
AACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCT78260.5461140489339401No Hit
CTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCATCGTTTA75720.528389417138742No Hit
GATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGA72860.5084317608654086No Hit
GTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGA68140.4754946498129144No Hit
ACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGT66610.4648180015268305No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTT64780.45204789279249485No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTGC62390.4353699912214225No Hit
CGGTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGG54950.3834521721047791No Hit
CTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCG54000.3768228806853152No Hit
GTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTT53350.3722870497141031No Hit
GCATTGGAAACTGACAGACTTGAATGTAGAAGAGGAAAATGGAATTCCAA53300.3719381396393944No Hit
GGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACT53060.37026337128079306No Hit
CGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAG52320.3650995021751054No Hit
GCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAGAG48540.33872190052713336No Hit
GTAGAAACACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAG48410.33781473433289094No Hit
GAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTT48330.33725647821335714No Hit
TATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCTCTTCTACA45690.3188340262687417No Hit
GCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACGG45040.3142981952975296No Hit
CTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCT42990.29999288223447595No Hit
GCGTAGATATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAA42830.29887636999540834No Hit
ATGTTAGACCAGAAAATCGCCTTCGCCACTGGTGTTCCTCCAAATATCTA41430.28910688790356687No Hit
GTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTA41290.28812993969438266No Hit
CTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGTCCGGAT40550.28296607058869505No Hit
CCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCC40230.2807330461105598No Hit
GGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGT39000.2721498582727277No Hit
GGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACG38970.2719405122279025No Hit
CACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA38690.2699866158095342No Hit
GTCGTAATCCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGG38690.2699866158095342No Hit
CCCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCT38360.26768380931645724No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT37910.2645436186440796No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT37870.26426449058431273No Hit
GTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACT35060.244655744385688No Hit
GGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATG34370.23984078535470899No Hit
ATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGC34310.2394220932650586No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG33950.2369099407271565No Hit
ATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCT33320.23251367378582782No Hit
GAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACC32670.2279778428146157No Hit
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGTC32530.22700089460543157No Hit
GCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATT31670.22099964132044322No Hit
GGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGG31590.2204413852009094No Hit
GCGTCAATGTTAGACCAGAAAATCGCCTTCGCCACTGGTGTTCCTCCAAA31460.21953421900666698No Hit
CTTGAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCG31410.21918530893195837No Hit
CACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA31160.21744075855841521No Hit
GTGTAGCGGTGGAATGCGTAGATATTTGGAGGAACACCAGTGGCGAAGGC30330.21164885131825206No Hit
GACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGG30090.20997408295965067No Hit
GACTTGAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATG29590.20648498221256437No Hit
TAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGC29530.20606629012291403No Hit
AGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTG28740.20055351094251778No Hit
GTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAA28710.20034416489769258No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA27960.1951105137770632No Hit
GTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATG27030.18862078638748278No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA26800.1870158000438231No Hit
CTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAATCGCCTTCGCCACTG26380.18408495541627065No Hit
GTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAAT26360.18394539138638719No Hit
CGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCAT26290.1834569172817951No Hit
ATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAG25860.18045629063930094No Hit
GCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGT25650.17899086832552474No Hit
TCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACG25610.1787117402657578No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA24570.17145441071181844No Hit
CCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAG23820.16622075959118907No Hit
CATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCA23640.16496468332223801No Hit
CCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTGGGGGCTTTC23500.16398773511305384No Hit
TCCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAA23290.16252231279927762No Hit
CGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTG23200.16189427466480208No Hit
ATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACG23040.1607777624257345No Hit
ATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAGAT22890.1597310322016086No Hit
GATATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTG22880.1596612501866669No Hit
GTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGG22870.15959146817172515No Hit
TTCCACCGCTACACTTGGAATTCCATTTTCCTCTTCTACATTCAAGTCTG22860.15952168615678344No Hit
CCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTA22610.15777713578324032No Hit
GTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTGCGGTCGTAATCC22510.15707931563382305No Hit
GTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGT22470.15680018757405617No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA22050.15386934294650373No Hit
GTGCTAAGTGTTGGGGGCTTTCCGGCCCTCGGTGCTGGAGCTAACGTATT22000.1535204328717951No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA21950.15317152279708646No Hit
GCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAG21900.15282261272237782No Hit
CATTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGGATTAGATACCC21230.14814721772128225No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTG21200.14793787167645708No Hit
ACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCC21070.14703070548221464No Hit
CCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAGAGGAAAAT21030.14675157742244777No Hit
AGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGC20760.1448674630190212No Hit
CCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCA20370.1421459644362939No Hit
TATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATA20290.14158770831676012No Hit
CCAAATATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCTCTT20190.14088988816734285No Hit
AGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACAT19830.13837773562944075No Hit
GGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAGATATTTGGAGGA19700.1374705694351983No Hit
GACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTG19310.13474907085247104No Hit
CCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAATCGCCTTC19150.13363255861340345No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC19120.13342321256857828No Hit
CACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACGCTGAGGCTCGA19060.1330045204789279No Hit
CGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACGGC18950.13223691831456894No Hit
CCTCCAAATATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCT18870.13167866219503516No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA18640.13007367585137547No Hit
TTCCAATGCCGTTCCACGGTTAAGCCGTGGGCTTTCACATTAGACTAAAC18620.129934111821492No Hit
CCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACGCTGAGGCTCGAAA18460.12881759958242442No Hit
ATTAGTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAA18440.12867803555254095No Hit
GTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTT18350.12804999741806544No Hit
ACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAG18260.1274219592835899No Hit
GTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGC18250.1273521772686482No Hit
AATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTG18230.12721261323876473No Hit
GTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATACGTTAGC18090.1262356650295806No Hit
ACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTC18070.12609610099969715No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT17680.12337460241696988No Hit
GTCTAACATTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGGATTAG17630.12302569234226124No Hit
GAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATACGTTA17560.12253721823766917No Hit
GGTCTGTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCAT17450.12176961607331019No Hit
GAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAG17420.12156027002848502No Hit
AGATTATACTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA17130.11953659159517499No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC17070.11911789950552465No Hit
ACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCT17040.11890855346069946No Hit
GCGGTGGAATGCGTAGATATTTGGAGGAACACCAGTGGCGAAGGCGATTT17000.11862942540093256No Hit
TATTAGTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAA16990.11855964338599084No Hit
AACACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGT16960.11835029734116567No Hit
AACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG16780.11709422107221462No Hit
ACATTCAAGTCTGTCAGTTTCCAATGCCGTTCCACGGTTAAGCCGTGGGC16630.11604749084808874No Hit
GTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGG16250.11339577428030319No Hit
CCACTGGTGTTCCTCCAAATATCTACGCATTCCACCGCTACACTTGGAAT16190.11297708219065283No Hit
CTTTTGAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATA15760.10997645554815866No Hit
AGACTAAACAGACCGCCTGCGCTCCCTTTACGCCCAATAAATCCGGACAA15520.10830168718955727No Hit
CAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCC15450.10781321308496519No Hit
CGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAG15320.10690604689072276No Hit
ATTCTTTTGAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTA15230.10627800875624724No Hit
GCCCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATC15200.10606866271142205No Hit
CATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACC15170.10585931666659688No Hit
CCGCTACACTTGGAATTCCATTTTCCTCTTCTACATTCAAGTCTGTCAGT15100.10537084256200481No Hit
CTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCC15050.10502193248729619No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA15050.10502193248729619No Hit
ACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCA15030.10488236845741274No Hit
CGTAATCCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGGCC14960.10439389435282066No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA14870.10376585621834514No Hit
CTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTA14590.10181195979997683No Hit
GTTAAGCCGTGGGCTTTCACATTAGACTAAACAGACCGCCTGCGCTCCCT14440.10076522957585096No Hit
GGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGT14400.10048610151608406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCC202.1310589E-6144.99818145
GAGCGTG203.8665946E-4108.748647
TAGAAAC23800.0108.4629442
AAACACG24000.0103.02715
GAAACAC24300.0101.4567644
CACGCAC650.0100.400873
TAGTAAC26800.099.838932
GTAGAAA26100.099.4848561
AACGGCC301.5997965E-596.66546145
AGCACGC750.087.0353551
AACACGT9350.086.852926
ACACGTG9350.086.8438347
GTAACAA31050.086.406914
AACACGA19650.084.8678746
ACACGAG20900.079.783697
AGTAACA33850.079.259513
GCCTTCC851.8189894E-1276.76375145
GCACGCA903.6379788E-1272.511742
AGCGTGC300.001936010272.499098
CACGAGC23400.071.2597968
Produced by FastQC (version 0.11.5)