FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_1230_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_1230_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440937
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAG210341.4597445967450347No Hit
GTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAA190971.3253181783797627No Hit
GCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTA167811.1645894303498348No Hit
GTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAG136710.9487576486688869No Hit
CTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTG131610.91336401244468No Hit
GTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCAC128830.8940710107381517No Hit
GGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTG106110.7363958313236456No Hit
GTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAAT89460.6208460189446173No Hit
AGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAAC88230.6123099066787792No Hit
CACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTG86750.6020388122450877No Hit
GTAGAAACACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAG82900.5753200868601472No Hit
AACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCT78770.5466581814472111No Hit
CTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCATCGTTTA76050.5277815754609674No Hit
GATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGA73330.5089049694747237No Hit
GTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGA68580.47594030828551137No Hit
ACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGT66920.4644200266909657No Hit
GGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTT65150.45213635294256443No Hit
CTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTGC62690.4350641284108882No Hit
CGGTAATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGG55260.38350045838228874No Hit
CTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCG54270.3766299289975898No Hit
GCATTGGAAACTGACAGACTTGAATGTAGAAGAGGAAAATGGAATTCCAA53670.3724659717947419No Hit
GTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTT53630.37218837464788534No Hit
GGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACT53350.3702451946198897No Hit
CGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAG52580.3649014495429016No Hit
GCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAGAG48760.3383909220181035No Hit
GTAGAAACACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAG48720.33811332487124696No Hit
GAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTT48690.33790512701110453No Hit
TATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCTCTTCTACA45970.3190285210248609No Hit
GCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACGG45320.3145175673884424No Hit
CTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCT43160.29952732145819005No Hit
GCGTAGATATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAA43100.29911092573790526No Hit
ATGTTAGACCAGAAAATCGCCTTCGCCACTGGTGTTCCTCCAAATATCTA41640.2889786298776421No Hit
GTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTA41520.28814583843707253No Hit
CTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGTCCGGAT40810.28321848908036923No Hit
CCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCC40470.28085891333208873No Hit
GGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACG39220.2721840024928224No Hit
GGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGT39100.2713512110522528No Hit
GTCGTAATCCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGG38920.2701020238913984No Hit
CACGTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA38860.2696856281711137No Hit
CCCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCT38550.2675342502829756No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT38070.2642030845206973No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT38040.26399488666055493No Hit
GTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACT35280.24484068352745472No Hit
GGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATG34630.24032972989103618No Hit
ATACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGC34520.23956633773718075No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG34060.23637397054833068No Hit
ATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCT33550.23283460692591No Hit
GAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACC32790.22756026113563604No Hit
GCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGTC32680.2267968689817806No Hit
GCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATT31820.22082853032436534No Hit
GGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGG31800.22068973175093706No Hit
GCGTCAATGTTAGACCAGAAAATCGCCTTCGCCACTGGTGTTCCTCCAAA31560.2190241488697979No Hit
CTTGAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCG31550.21895474958308378No Hit
CACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCA31360.2176361631355153No Hit
GTGTAGCGGTGGAATGCGTAGATATTTGGAGGAACACCAGTGGCGAAGGC30490.21159842519138586No Hit
GACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGG30320.21041863731724567No Hit
GACTTGAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATG29790.2067404751213967No Hit
TAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGC29700.2061158815409695No Hit
AGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCATTGGAAACTG28860.20028634145698251No Hit
GTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAA28830.20007814359684012No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA28150.19535899210027918No Hit
GTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATG27250.1891130562960074No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA26930.1868922791211552No Hit
CTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAATCGCCTTCGCCACTG26590.18453270337287472No Hit
GTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAAT26520.1840469083658758No Hit
CGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCAT26430.18342231478544863No Hit
ATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAG26050.18078514189031164No Hit
TCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACG25810.1791195590091725No Hit
GCCCACGGCTTAACCGTGGAACGGCATTGGAAACTGACAGACTTGAATGT25760.17877256257560184No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA24710.1714856374706181No Hit
CCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAAG23960.16628069096705822No Hit
CATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCA23750.16482330594606148No Hit
CCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTGGGGGCTTTC23610.16385171593206366No Hit
TCCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGGCCGGAAA23410.16246373019778104No Hit
CGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTG23330.16190853590406798No Hit
ATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACG23210.1610757444634984No Hit
GTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGG23060.16003475516278642No Hit
ATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAGAT23050.15996535587607233No Hit
TTCCACCGCTACACTTGGAATTCCATTTTCCTCTTCTACATTCAAGTCTG23020.1597571580159299No Hit
GATATTTGGAGGAACACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTG22980.1594795608690734No Hit
CCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTA22710.15760578012779183No Hit
GTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTGCGGTCGTAATCC22650.15718938440750707No Hit
GTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGT22560.15656479082707986No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA22110.153441822924944No Hit
GTGCTAAGTGTTGGGGGCTTTCCGGCCCTCGGTGCTGGAGCTAACGTATT22090.15330302435151572No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA22060.15309482649137332No Hit
GCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTGTTTAG21980.15253963219766029No Hit
CATTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGGATTAGATACCC21340.1480980778479559No Hit
GCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACCTTG21320.14795927927452762No Hit
ACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCC21240.1474040849808146No Hit
CCGTGGAACGGCATTGGAAACTGACAGACTTGAATGTAGAAGAGGAAAAT21160.14684889068710152No Hit
AGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGC20860.1447669120856776No Hit
CCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCA20490.1421991384772547No Hit
TATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATA20370.14136634703668516No Hit
CCAAATATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCTCTT20300.14088055202968625No Hit
AGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACAT19940.13838217770797753No Hit
GGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAGATATTTGGAGGA19770.13720238983383728No Hit
GACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAGGCGGTCTG19400.13463461622541445No Hit
CCCCACGCTTTCGAGCCTCAGCGTCAATGTTAGACCAGAAAATCGCCTTC19250.13359362692470247No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC19200.1332466304911318No Hit
CACCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACGCTGAGGCTCGA19130.1327608354841329No Hit
CGTAAAGGGAGCGCAGGCGGTCTGTTTAGTCTAATGTGAAAGCCCACGGC19070.13234443976384813No Hit
CCTCCAAATATCTACGCATTCCACCGCTACACTTGGAATTCCATTTTCCT18970.1316504468967068No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA18740.1300542633022818No Hit
TTCCAATGCCGTTCCACGGTTAAGCCGTGGGCTTTCACATTAGACTAAAC18690.12970726686871112No Hit
CCAGTGGCGAAGGCGATTTTCTGGTCTAACATTGACGCTGAGGCTCGAAA18560.12880507614142742No Hit
ATTAGTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAA18530.12859687828128502No Hit
GTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAATGTT18470.12818048256100023No Hit
GTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGC18400.12769468755400132No Hit
ACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCAG18380.12755588898057307No Hit
AATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTG18320.12713949326028826No Hit
ACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTC18190.12623730253300455No Hit
GTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATACGTTAGC18190.12623730253300455No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT17790.12346133106443931No Hit
GTCTAACATTGACGCTGAGGCTCGAAAGCGTGGGGAGCGAACAGGATTAG17730.12304493534415453No Hit
GAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATACGTTA17660.1225591403371556No Hit
GGTCTGTTTAGTCTAATGTGAAAGCCCACGGCTTAACCGTGGAACGGCAT17560.1218651474700143No Hit
GAATGTAGAAGAGGAAAATGGAATTCCAAGTGTAGCGGTGGAATGCGTAG17530.1216569496098719No Hit
AGATTATACTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA17220.11950557172173384No Hit
ACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCT17190.11929737386159145No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC17140.11895037742802081No Hit
TATTAGTAGTAACAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAA17100.11867278028116428No Hit
AACACGAGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGT17070.11846458242102187No Hit
GCGGTGGAATGCGTAGATATTTGGAGGAACACCAGTGGCGAAGGCGATTT17070.11846458242102187No Hit
AACCAGAAAGTCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAG16860.11700719740002513No Hit
ACATTCAAGTCTGTCAGTTTCCAATGCCGTTCCACGGTTAAGCCGTGGGC16710.11596620809931316No Hit
GTGCTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGG16370.1136066323510327No Hit
CCACTGGTGTTCCTCCAAATATCTACGCATTCCACCGCTACACTTGGAAT16300.11312083734403378No Hit
CTTTTGAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTAATA15880.11020606730204026No Hit
AGACTAAACAGACCGCCTGCGCTCCCTTTACGCCCAATAAATCCGGACAA15620.10840168584747285No Hit
CAAGAGCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCC15510.10763829369361741No Hit
CGCCTTGACGGTATCTAACCAGAAAGTCACGGCTAACTACGTGCCAGCAG15410.1069443008264761No Hit
ATTCTTTTGAGTTTCAACCTTGCGGTCGTAATCCCCAGGCGGAGTGCTTA15340.1064585058194772No Hit
GCCCCCAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATC15300.10618090867262067No Hit
CCGCTACACTTGGAATTCCATTTTCCTCTTCTACATTCAAGTCTGTCAGT15270.10597271081247826No Hit
CATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCTTTTGAGTTTCAACC15250.10583391223905No Hit
ACGTTAGCTCCAGCACCGAGGGCCGGAAAGCCCCCAACACTTAGCACTCA15150.1051399193719087No Hit
TACGTAGGTGACAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA15140.10507052008519456No Hit
CTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCC15100.10479292293833803No Hit
CGTAATCCCCAGGCGGAGTGCTTAATACGTTAGCTCCAGCACCGAGGGCC15030.10430712793133912No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA14970.10389073221105433No Hit
CTAGGTAACTACTAGCGCCTTGACGGTATCTAACCAGAAAGTCACGGCTA14650.10166995503620214No Hit
GTTAAGCCGTGGGCTTTCACATTAGACTAAACAGACCGCCTGCGCTCCCT14500.10062896573549017No Hit
GGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGT14470.10042076787534777No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC100.0070898947145.008825
ACCGTTA100.0070913658144.998769
ACCCGCC100.0070913658144.99876145
TAGAAAC24300.0107.1232
AAACACG23950.0105.65365
GCCTTCC900.0104.72132145
TAGTAAC26050.0104.658532
GAAACAC24250.0104.047564
AGCACGC1000.0101.523791
GTAGAAA26050.099.9370351
AACGGCC301.5997686E-596.66583145
CACGCAC1100.092.2847443
GTAACAA30700.088.327844
AACACGA19150.087.081026
GCACGCA1200.084.5943452
AGTAACA32850.083.435523
AACACGT10600.082.7644656
ACACGTG10850.080.851847
ACACGAG20700.080.554867
AACAACG3000.077.3326647
Produced by FastQC (version 0.11.5)