FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l01_n01_SM2_1210_P1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_1210_P1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809675
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT61830.3416635583737412No Hit
GTGTAAATACAATAAGTAATGAGGAAGCGAGTAACGAGTTACAGAAAACC58220.321715225109481No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT39820.22003950985674225No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTACAAAACCAAA36390.20108583032865018No Hit
GATATATCCCTTAGTCTAGATATTGTCTGTCCAAGTTTAAAATCAATTCC35570.1965546299749955No Hit
GTCTAGATATTGTCTGTCCAAGTTTAAAATCAATTCCCTTTTCAACATCT33350.184287233895589No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT32410.1790929310511556No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC31010.17135673532540374No Hit
GTCCAGAGATCCTTAGCTCAAGAGCATGGAATAGAGACTCCCAAGTATCT30870.17058311575282856No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA27240.15052426540677194No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT26880.14853495793443575No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCGGCTGG24810.13709643996850263No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG24610.13599126915053808No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA24600.13593601060963986No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC24470.1352176495779629No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA24410.1348860983325735No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT24410.1348860983325735No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA24310.13433351292359125No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAATTT24240.13394670313730367No Hit
GTACTACTAAATTCAAAAGTTACAACTGTTACAACATCAGTATCTCCCCT23540.13007860527442772No Hit
ATGTTAATCCATCCCCTGTGGCCCATTTGGCATAATTGGTTTTCTGTAAC23520.12996808819263128No Hit
TAGTAGTACAGATCCCAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG23410.12936024424275078No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC23330.12891817591556495No Hit
ATTTCCTTTTATTCATCCATTACTTCATCCACTGATTCCAACACTTTACT22930.12670783427963583No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCTGAGTCCACTCTGGGATC22570.12471852680729965No Hit
CTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTTCTCGCTTTTCATTAT22480.12422119993921561No Hit
CCCGTAGAGCATGTAGGAAGTGAAAGAAGGAAATACTTTGTCAACGAAAT21610.11941370688106981No Hit
TCCAAATACATAGTGCATCAAGCATTTAGTAAATATTACTCTTAGTGCTT20940.11571138464088856No Hit
GGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCG20900.11549035047729564No Hit
CTCTACGGGTGCTATGTCTGGCCCTATTTCTGGTAGGTCCAGAGCTCTTT20820.11504828215010983No Hit
CTCCTAGACTCGGCACTAAACCCCTCCAATTGGGCATTTCCAAATACATA20640.11405362841394172No Hit
ATATGGAACTTCTGGTAGCTCATGGTCGTAAATGTGAGGCCCTATTGGTC20260.11195380385980909No Hit
TATCTAGACTAAGGGATATATCTGTTCCAGCCGGTTTCTCCAATTTTGAA20090.11101440866453922No Hit
GCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCACAATATGAAGT20050.1107933745009463No Hit
GATTAACATACCAGAAAATAATGAAAGAAGTAGCAATAGATGACGAAACA20020.11062759887825163No Hit
ATATAATGCCTTTCTTCTAATGTCTGATGAACTAGGATTGGCCAATATGA19840.10963294514208352No Hit
GATTTATAGAAGTTGGAATAACAAAGGGATTGGCTGATGATTACTTTTGG19520.10786467183334025No Hit
ATTCAATATCTGCGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA19220.1062069156063934No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC19050.10526752041112354No Hit
CCCAAGTATCTGGCTGATTTGTTTGATTACAAAACCAAAAGATTTATAGA18870.10427286667495546No Hit
CTTCTAATGTCTGATGAACTAGGATTGGCCAATATGACTGAGGGAAAGTC18330.10128890546645115No Hit
GTGTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTA18320.10123364692555294No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACG100.0070874915145.026284
GAGAGCG100.007091591144.998257
CTAACTG100.007091591144.99825145
ACGTGCA2150.0101.161569
AACACGT3100.095.904476
GAAACAC6000.095.475644
AAACACG6200.092.3957755
CACGTGC3150.092.062388
ACACGTG3400.087.425417
ACGTGCC801.8189894E-1281.561519
TAGAAAC7250.081.014682
AACACGA3450.079.8695456
CTACCAT651.5661499E-978.075979
ACACGAG3850.071.557577
CACGAGC3950.069.745988
AGAAGCG2100.062.1541183
AAGCGGA1800.060.427622
AGCGGAG1800.060.427623
ACGAGCC1700.059.7051549
GAAGCGG3550.059.2491841
Produced by FastQC (version 0.11.5)