FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_1210_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_1210_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816951
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT61990.34117595906548936No Hit
GTGTAAATACAATAAGTAATGAGGAAGCGAGTAACGAGTTACAGAAAACC58460.32174780717806917No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT39940.21981880634095252No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTACAAAACCAAA36530.20105110154318967No Hit
GATATATCCCTTAGTCTAGATATTGTCTGTCCAAGTTTAAAATCAATTCC35680.19637293465811684No Hit
GTCTAGATATTGTCTGTCCAAGTTTAAAATCAATTCCCTTTTCAACATCT33480.18426473801439885No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT32560.17920131032702588No Hit
ATATAGACCCAAAAGGAGCAATAGAGAGAAATCTAGCAAGGATGTCTCCC31100.17116587073619488No Hit
GTCCAGAGATCCTTAGCTCAAGAGCATGGAATAGAGACTCCCAAGTATCT30980.1705054236465375No Hit
GCTTAGCACTCTCCCTTTCCCTTCCTCATCCAATGAGGATTCATTACTTA27340.15047186192693143No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACT26960.14838044614301651No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCGGCTGG24910.13709780836137023No Hit
TCCTTGTATTGATGATTCCTGTTCAACAATTGCCTCCATTTGTTGCATTG24680.13583195143952698No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA24640.1356118024096412No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC24590.13533661612228398No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT24510.1348963180625124No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA24500.134841280805041No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA24360.13407075920044073No Hit
GTGCTAAGCAGACTCACAGAACTTCAGGCTGAATTAAGTCTGAAAAATTT24300.13374053565561206No Hit
ATGTTAATCCATCCCCTGTGGCCCATTTGGCATAATTGGTTTTCTGTAAC23650.13016311391996813No Hit
GTACTACTAAATTCAAAAGTTACAACTGTTACAACATCAGTATCTCCCCT23640.13010807666249666No Hit
TAGTAGTACAGATCCCAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG23520.12944762957283934No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC23430.12895229425559632No Hit
ATTTCCTTTTATTCATCCATTACTTCATCCACTGATTCCAACACTTTACT23060.12691591572915284No Hit
ATCCTAAACACAGTATATTTTGGCCACTTTCCTGAGTCCACTCTGGGATC22610.12443923914293781No Hit
CTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTTCTCGCTTTTCATTAT22580.12427412737052349No Hit
CCCGTAGAGCATGTAGGAAGTGAAAGAAGGAAATACTTTGTCAACGAAAT21790.1199261840302793No Hit
TCCAAATACATAGTGCATCAAGCATTTAGTAAATATTACTCTTAGTGCTT21020.11568831520497802No Hit
GGTCTATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCG20940.11524801714520645No Hit
CTCTACGGGTGCTATGTCTGGCCCTATTTCTGGTAGGTCCAGAGCTCTTT20930.115192979887735No Hit
CTCCTAGACTCGGCACTAAACCCCTCCAATTGGGCATTTCCAAATACATA20700.11392712296589176No Hit
ATATGGAACTTCTGGTAGCTCATGGTCGTAAATGTGAGGCCCTATTGGTC20390.11222096798427697No Hit
TATCTAGACTAAGGGATATATCTGTTCCAGCCGGTTTCTCCAATTTTGAA20210.11123029734979094No Hit
GATTAACATACCAGAAAATAATGAAAGAAGTAGCAATAGATGACGAAACA20130.11078999929001938No Hit
GCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCACAATATGAAGT20120.11073496203254793No Hit
ATATAATGCCTTTCTTCTAATGTCTGATGAACTAGGATTGGCCAATATGA19880.10941406785323325No Hit
GATTTATAGAAGTTGGAATAACAAAGGGATTGGCTGATGATTACTTTTGG19560.10765287561414699No Hit
ATTCAATATCTGCGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA19260.10600175789000363No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC19110.10517619902793195No Hit
CCCAAGTATCTGGCTGATTTGTTTGATTACAAAACCAAAAGATTTATAGA18940.10424056565091738No Hit
CTTCTAATGTCTGATGAACTAGGATTGGCCAATATGACTGAGGGAAAGTC18410.1013235910049308No Hit
GTGTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTA18370.10110344197504502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCC1050.0117.379719
AACACGT2650.0112.177886
AAACACG5400.0104.735525
GAAACAC5450.0103.774644
CACGTGC3000.099.0822758
AACACGA3050.090.3343666
ACGTGCA2000.086.999079
ACACGAG3200.086.0928347
ACACGTG3500.084.927677
CAACGCA608.2945917E-1084.582439
CACGAGC3450.077.75288
TAGAAAC7400.077.4127352
ACGAGCC1400.067.320719
AGCGTTT554.063195E-665.908398
TCGATCT400.00604863954.381914
CGATCTG400.00604863954.381915
TTAAGCG400.006051932554.3744249
TTATATA553.2168112E-452.7368742
GAGCATC701.6966454E-551.7851647
GTAACAA5000.050.7564434
Produced by FastQC (version 0.11.5)