FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l01_n01_SM2_1208_P1_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l01_n01_SM2_1208_P1_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578780
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT41890.26533145846793094No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT30950.19603744663601008No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA30440.19280710421971395No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT29030.18387615753936584No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC27680.17532525114328784No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA27530.17437515043261254No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC27420.17367840991145062No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA27070.17146150825320816No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGTTACA25150.15930021915656392No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT24420.15467639569794397No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA24300.15391631512940374No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA22930.14523872863856901No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGA22920.14517538859119067No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA21200.13428090044211352No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC21080.13352081987357325No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA21070.1334574798261949No Hit
GTGCTATACTACAAAAGTCCCTGAACCAAACTGGGCTGTCTCTGGTTATT20660.13086053788368235No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC19910.12611003433030568No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA19710.12484323338273857No Hit
AGATTATACTTCAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA19280.12211961134546928No Hit
GTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAA19220.12173957106119916No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAGAGAAAGCTGAA18830.1192693092134433No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA18080.11451880566006663No Hit
TTCTAATGGTATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC18080.11451880566006663No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC17900.11337868480725624No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC17800.11274528433347268No Hit
GTATAGATCTGTTTCTTTTGGTTCTCCAACTATGAGTTCCAGACACCGCA17540.11109844310163543No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC17290.10951494191717656No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA17260.10932492177504148No Hit
ACCTAAAAGAGGTTATTGTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTT17080.1081848009222311No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT16930.10723470021155575No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT16800.10641127959563715No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG16790.10634793954825879No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT16630.10533449879020508No Hit
TGTTTATTCCCAATAATTTACATGTTCGGTAAAAGTCGTTTATTCCTTCC16490.10444773812690812No Hit
GTCTAATGAATCAATGATATCCTGGCAAAAAGGTATTAATCCACCTTTGT16380.10375099760574621No Hit
ATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAAATCCC16290.10318093717934101No Hit
GTCCTGATCTGTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGG15940.10096403552109856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATTA100.007084147145.04861
ACTAATG100.007091531144.998068
AACTAAT100.007091531144.998067
GTAACAA7600.093.5004044
GAAACAC5950.092.618474
AAACACG6000.091.846665
AGCACGC406.1069295E-790.655371
CACGCAC406.1126957E-790.6410143
GCACGCA406.1126957E-790.6410142
GAGCAGA1400.088.065221
GGTGCGT353.435962E-582.866546
AACACGA4900.079.907016
GCAGGTC1750.078.7132348
ACACGAG5050.077.523717
TAGAAAC7400.077.412322
CGTGCTA406.658549E-572.512813
CGGTGCA608.0248356E-872.512812
CAACGCA451.19415025E-464.443599
TGCGATA1350.064.44359145
GAACCAC350.00356707462.142038
Produced by FastQC (version 0.11.5)