FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33051797
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23266577.0394266308727484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG235900.025.101523
ATCGGAA244850.024.6264692
CGGAAGA244450.024.4402564
GATCGGA244350.024.0633831
TCGTGTA136800.019.89164414-15
GTAGGGA133450.017.20753318-19
AGCGTCG162500.016.9614610-11
GAGCGTC330950.016.9415639
TAGGGAA135750.016.51364118-19
AGAGCGT364700.015.6454168
AAGAGCG389500.015.2334377
TGTAGGG146300.015.07830116-17
GTGTAGG155500.014.75121416-17
CGTGTAT24250.013.70845114-15
AGGGAAA202900.012.73414320-21
GGAAGAG523200.012.1456245
GAAGAGC515850.011.8142086
ATTACGA23800.011.17634954-55
CGTGTAA17850.010.10991514-15
CGTGTAG252450.09.03899514-15
Produced by FastQC (version 0.11.5)