FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24301960
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG134600.013.9401950-51
AAAAGGG372400.012.41741880-81
AAGGGGG373850.012.356729582-83
AAAGGGG364550.011.86391180-81
TATAGGG71800.010.45335148-49
AAAAAGG515550.010.023952578-79
GGGAAAA346950.09.90440522-23
TAAAAAA888500.09.69854670-71
GGGGGAA402900.09.58402120-21
CGGGGTT81700.09.41673851
GGGGAAT167550.09.18417962-63
AAAAAAG789800.08.89471278-79
GGCGAAT33350.08.6864341
TTTAGGG119950.08.35612948-49
CGGGGAT68300.08.3438811
AGGGGGG607550.08.31899582-83
GGGGGGA633600.08.30167360-61
CTCGGGT69900.08.2208311
GGGAATA122500.08.20003764-65
CTCGAAT52550.08.0431261
Produced by FastQC (version 0.11.5)