FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25088313
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG254390.10139781020748585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG336550.011.72904680-81
CGGGGAT71550.010.1545871
CGCGATT9701.4915713E-109.7912641
TAGGGGG134400.09.27792550-51
AAAAAGG479100.09.21505978-79
CGCGGAT37200.09.1911541
AAAAGGG335250.08.86203578-79
AGGGGGG631600.08.5440182-83
AAGGGGG354850.08.35316480-81
CTCGGAT69850.08.2262031
TAAAAAA762450.08.11884170-71
CCGGGAT76200.07.85228441
AAAAAAG710350.07.722648676-77
GGGAAAT207650.07.638271
GATCGGA420950.07.61003294-95
TCGCGTC21350.07.55988552
CGCGGGT51200.07.51269151
CGCGTAT10252.8930463E-67.41270351
CGGGAAT61750.07.38269231
CGGGAAC85300.07.34861371
Produced by FastQC (version 0.11.5)