FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32004218
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG474100.010.89112180-81
AAAGGGG469600.010.631330580-81
AAGGGGG511850.010.12061282-83
TAGGGGG167150.09.30727950-51
CGGGGAT97200.09.185091
AAAAAGG651150.09.07096878-79
TAAAAAA1068350.08.48358770-71
CGGGAAT78950.08.3609281
CCGGAAT61350.08.3598991
CGGAAAT69100.08.2469811
GGGGGAA499600.07.985590520-21
CCGGGAT90450.07.8754341
AAAAAAG989950.07.83061378-79
CGGGCAT113950.07.6265581
GCGGTAT34700.07.3901981
CTCGGAT84400.07.3709021
GTCGGTT55600.07.34541
GGGAAAA444500.07.33006222-23
CGCGGAT43450.07.3227941
GCGTCGT244050.07.26883394-95
Produced by FastQC (version 0.11.5)