FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24631633
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG331250.012.05306680-81
TAGGGGG131650.010.51809150-51
AAGGGGG347800.09.74519882-83
AAAAAGG481300.09.49397478-79
CGGGAAT55950.09.2518541
AAAAGGG344250.09.121016580-81
AGGGGGG568600.09.09802982-83
CGGGGAT74850.09.0729091
TAAAAAA791250.08.33364470-71
CGCGGAT34150.08.0656551
CCGGTAT27950.07.98579931
CGCGGGT49000.07.947311
GCGGGAT58200.07.9150041
CGGGAAC81600.07.85680441
GAAATCG63000.07.61066533
CGCAATC26200.07.61013752
CGGGCAT85750.07.5873271
CGGAAAT52700.07.5695671
GGGGGGA568600.07.48859720-21
GCGTCGT190200.07.441373394-95
Produced by FastQC (version 0.11.5)